Ethanol-Induced Effects on Sting Extension Response and Punishment Learning in the Western Honey Bee (Apis mellifera)

Giannoni-Guzmán, Manuel A.; Giray, Tuğrul; Agosto‐Rivera, José L.; Stevison, Blake K.; Freeman, B.; Ricci, Paige; Brown, Erika A.; Abramson, Charles I.

doi:10.1371/journal.pone.0100894

Cited by 29 publications

(48 citation statements)

References 74 publications

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“…Pszczoły okazały się doskonałym obiektem do badań społecznych aspektów spożywania alkoholu ze względu na to, że są zwierzętami mającymi zdolność porozumiewania się i uczenia, a zmiany w ich skomplikowanym behawiorze są łatwe do zauważenia (9). Ponadto pszczeli model do badania alkoholizmu jest szczególnie istotny, ponieważ owady te mają kontakt z naturalnymi źródłami alkoholu w naturze w postaci sfermentowanego nektaru lub soków roślinnych (41,101). Potwierdzono, że alkohol negatywnie wpływa na pszczoły -"pijące" robotnice są niezdolne do pracy, nie uczą się, poruszają się w sposób nieskoordynowany, a zbieraczki nie wracają do ula po wylocie w celu zdobycia pokarmu (7).…”

Section: Pszczoły Jako Model Do Badania Wpływu Używekunclassified

“…Spożywanie alkoholu powoduje przesunięcie granicy progu wrażliwości na bodźce (Sting Extinsion Response (SER)) -co może wskazywać na działanie przeciwbólowe. Upojenie alkoholowe pszczół wywołuje brak zahamowań w zachowaniu, zakłóca kontrolę nad żądleniem oraz kojarzenie znaczenia bodźców pochodzących ze środowiska (41). Ponadto zachowania higieniczne całej rodziny również ulegają zakłóceniu (9).…”

Section: Pszczoły Jako Model Do Badania Wpływu Używekunclassified

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Honey bee as an alternative model invertebrate organism

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2025

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Insects perfectly fit the flagship principle of animal research – 3R: to reduce (the number of animals), to replace (animals with alternative models) and to refine (methods). Bees have the most important advantages of a model organism: they cause minimal ethical controversy, they have a small and fully known genome, and they permit the use of many experimental techniques. Bees have a fully functional DNMT toolkit. Therefore, they are used as models in biomedical/genetic research, e.g. in research on the development of cancer or in the diagnostics of mental and neuroleptic diseases in humans. The reversion of aging processes in bees offers hope for progress in gerontology research. The cellular mechanisms of learning and memory coding, as well as the indicators of biochemical immunity parameters, are similar or analogous to those in humans, so bees may become useful in monitoring changes in behavior and metabolism. Bees are very well suited for studies on the dose of the substance applied to determine the lethal dose or the effect of a formula on life expectancy. Honeybees have proven to be an effective tool for studying the effects of a long-term consumption of stimulants, as well as for observing behavioral changes and developing addictions at the individual and social levels, as well as for investigating the effects of continuously delivering the same dose of a substance. The genomic and physiological flexibility of bees in dividing tasks among workers in a colony makes it possible to create a Single- Cohort Colony (SCC) in which peers compared perform different tasks. Moreover behavioral methods (e.g. Proboscis Extension Reflex – PER, Sting Extension Reflex – SER, free flying target discrimination tasks or the cap pushing response) make it possible to analyse changes occurring in honeybee brains during learning and remembering. Algorithms of actions are created based on the behavior of a colony or individual, e.g. Artificial Bee Colony Algorithm (ABCA). Honeybees are also model organisms for profiling the so-called intelligence of a swarm or collective intelligence. Additionally, they serve as models for guidance systems and aviation technologies. Bees have inspired important projects in robotics, such as B-droid, Robobee and The Green Brain Project. It has also been confirmed that the apian sense of smell can be used to detect explosive devices, such as TNT, or drugs (including heroin, cocaine, amphetamines and cannabis). This inconspicuous little insect can revolutionize the world of science and contribute to the solution of many scientific problems as a versatile model.

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Section: Pszczoły Jako Model Do Badania Wpływu Używekunclassified

Honey bee as an alternative model invertebrate organism

Łoś¹,

Bieńkowska²,

Strachecka³

2025

Medycyna Weterynaryjna

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“…Several studies have established that honey bees will willingly consume EtOH solutions that contain sucrose, even at levels of EtOH >10%. These studies have included bees that were restrained and fed specific doses (e.g., see Abramson et al., ; Giannoni‐Guzman et al., ; Maze et al., ; Mustard et al., ) or free flying bees that were trained to a feeder, which was then swapped out for one containing EtOH (Bozic et al., ; Sokolowski et al., ). However, whether or not honey bees prefer to consume EtOH using a 2‐feeder choice assay similar to that used to measure preference in rodent and Drosophila models has not yet been determined.…”

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Honey Bees (Apis mellifera) Show a Preference for the Consumption of Ethanol

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Alcoholism Clin & Exp Res

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Background: Alcohol abuse and alcoholism are significant global issues. Honey bees are excellent models for learning and other complex behaviors; furthermore, they share many behavioral responses to ethanol (EtOH) with humans and animal models. We develop a 2-feeder choice assay to determine whether honey bees will self-administer and preferentially consume solutions containing EtOH.Methods: Gustatory responsiveness to EtOH is determined using the proboscis extension reflex and consumption assays. A 2-feeder choice assay is used to examine preference for the consumption of EtOH. Survival assays assess the metabolic and toxic effects of EtOH consumption.Results: Honey bees find the taste of EtOH to be aversive when in water, but addition of sucrose masks the aversive taste. Even though the taste of EtOH is not appetitive, honey bees preferentially consume sucrose solutions containing 1.25 to 2.5% EtOH in a dose-dependent manner. Based on survival assays, honey bees may not be able to derive caloric value from EtOH, and EtOH concentrations of 2.5% or higher lead to significant increases in mortality.Conclusions: Honey bees will self-administer EtOH and show a preference for consuming solutions containing EtOH. Bees may not be able to efficiently utilize EtOH as an energy source, but EtOHdependent increases in mortality complicate separating the effects of caloric value and toxicity.

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“…This would be similar to comparing bees treated orally with ethanol and control group bees. For these two groups, both in appetitive and aversive learning tasks the 10% or higher ethanol treatment group performed poorly (Giannoni-Guzmán et al, 2014). However, in a supplemental study we demonstrated in an aversive learning paradigm, ESA conditioning, both A.m. syriaca and A.m. caucasica demonstrated complete reversal of punishment learning.…”

Section: Appetitive Vs Aversive Learningmentioning

confidence: 67%

“…We used a shuttle box apparatus as described before (Agarwal et al, 2011;Giannoni-Guzmán et al, 2014). The shuttle box measured 15 cm long by 2 cm wide and contained an electric shock grid with wires spaced .35 cm apart.…”

Section: Supplemental Electric Shock Avoidance Assay (Esa)mentioning

confidence: 99%

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Supplemental Information 2: Electric Shock Avoidance Data for Two Honeybee Subspecies During a Reversal Learning Test

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We aimed to examine mechanistically the observed foraging differences across two honey bee, Apis mellifera, subspecies using the Proboscis Extension Response (PER) assay. Specifically, we compared differences in appetitive reversal learning ability between honey bee subspecies:Apis mellifera caucasica(Pollman), andApis mellifera syriaca(Skorikov) in a "common garden" apiary. It was hypothesized that specific learning differences could explain previously observed foraging behavior differences of these subspecies: A.m. caucasica switches between different flower color morphs in response to reward variability, and A.m. syriaca does not switch. We suggest that flower constancy allows reduced exposure by minimizing search and handling time, whereas plasticity is important when maximizing harvest in preparation for long winter is at a premium. In the initial or Acquisition phase of the test we examined specifically discrimination learning, where bees were trained to respond to a paired conditioned stimulus with an unconditioned stimulus and not to respond to a second conditioned stimulus that is not followed by an unconditioned stimulus. We found no significant differences among the subspecies in the Acquisition phase in appetitive learning. During the second, Reversal phase of the experiment, where flexibility in association was tested, the paired and unpaired conditioned stimuli were reversed. During the Reversal phaseA. mellifera syriacashowed a reduced ability to learn the reverse association in the appetitive learning task. This observation is consistent with the hypothesis that A.m. syriaca foragers cannot change the foraging choice because of lack of flexibility in appetitive associations under changing contingencies. Interestingly, both subspecies continued responding to the previously rewarded conditioned stimulus in the reversal phase. We discuss potential ecological

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Ethanol-Induced Effects on Sting Extension Response and Punishment Learning in the Western Honey Bee (Apis mellifera)

Cited by 29 publications

References 74 publications

Honey bee as an alternative model invertebrate organism

Honey bee as an alternative model invertebrate organism

Honey Bees (Apis mellifera) Show a Preference for the Consumption of Ethanol

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