Ethanol-induced changes in expression profiles of cell growth, fatty acid and desaturase genes of Mucor rouxii

Effect of melatonin on proliferation of neonatal cord blood mononuclear cells

“…5% ethanol showed an inhibitory effect on the Mucor rouxii mycelial growth at all stages, whereas Mucor rouxii was tolerant to low ethanol concentrations (about 1%-3%). [21] The fi nal concentration of ethanol in 50 ng/ml MLT cultures was just 0.5‰, which was much lower in the other groups. The exact effect of ethanol on lymphocytes awaits further study.…”

Differential expression of desaturase genes and changes in fatty acid composition ofMortierellasp. AGED in response to environmental factors

“…Ethanol at 2% (v/v) inhibited growth and 2.5% (v/v) ethanol inhibited both growth and lipid synthesis (Fig. ), while 1–3% (v/v) ethanol had little effect on biomass and the unsaturated/saturated fatty acid ratio and 5% (v/v) ethanol inhibited mycelial growth and down‐regulated desaturase genes in M. rouxii . The saturated fatty acid content decreased whereas the unsaturated fatty acid content increased when 1.5% (v/v) ethanol was added.…”

“…AGED were regulated at the transcript level in response to ethanol stress, resulting in changes in cell growth and fatty acid composition. Some researcher reported that ethanol suppressed cell division in the early stages of fermentation, affected membrane structural integrity and permeability and altered fatty acid composition . Owing to the complexity of stress responses and the involvement of various processes, physiological conditions and strain genetic background can affect the responsive mechanisms.…”

“…AGED fungal cultures were grown in basic PDB medium until harvest. To investigate the effect of temperature on lipid content and desaturase gene transcription, the cultures were incubated at different temperatures (20,25 and 30 ∘ C). The effect of ethanol on lipid content and desaturase gene transcription was assessed by adding different ethanol concentrations (0.5, 1.5, 2 and 2.5% v/v) into basic PDB medium at different times.…”

The codon-optimized Δ6-desaturase gene of Pythium sp. as an empowering tool for engineering n3/n6 polyunsaturated fatty acid biosynthesis

“…BCC53698, deposited in the BIOTEC culture collection (BCC), National Center for Genetic Engineering and Biotechnology, was used as the genetic resource for the isolation of a Δ 6 -desaturase gene. The fungus was grown in a semi-synthetic medium [36] at 30°C to logarithmic phase. S. cerevisiae DBY746 (MATα, his3-Δ1, leu2-3, leu2-112, ura3-52, trp1-289) was employed as a host for functional analysis of the cloned gene.…”