Ethanol-induced cell death in cultured rat astroglia

Protective Effects of Puerariae flos Against Ethanol-Induced Apoptosis on Human Neuroblastoma Cell Line SK-N-MC

“…DNA fragmentation assay revealed that EtOH-treated group exhibits the distinctive ladder pattern characteristic of apoptosis, while the PF pre-treated group showed a noticeable decreases in the intensity of EtOHinduced DNA laddering. These results demonstrated that EtOH induces apoptosis in human neuroblastoma cell line; this observation was similar to the previous reports (2,3,4,11) and PF shows protective action against EtOH-induced apoptosis. Recent reports indicate that caspases may play a role in neuronal cell death (12,13) as well as after neuronal injury (14).…”

“…Ethanol (EtOH) consumption during developmental central nervous system (CNS) leads to substantial neuronal loss in multiple brain regions (2) and results in serious symptoms including CNS dysfunction (3). However, the exact mechanism behind the induction of brain damage by EtOH is still elusive.…”

Effect of Puerariae radix on c-Fos Expression in Hippocampus of Alcohol-Intoxicated Juvenile Rats.

“…It has been reported that alcohol induces death in astroglia and neuroblastoma cells and that ethanol consumption during CNS development leads to substantial neuronal loss in multiple brain regions. [2][3][4] Electrophysiological studies suggest that alcohol disrupts learning and memory formation by compromising hippocampal functions. 16) At physiologically relevant concentrations, ethanol was shown to diminish the amplitude of stimulationevoked spikes in hippocampal slices, suggesting a suppressive effect of alcohol on cell activity.…”

“…Alcohol consumption is known to cause substantial neuronal loss in several regions of the brain, [2][3][4] and to exert various effects on the central nervous system (CNS). 5) It has been reported that alcohol induces death in a variety of cells including astroglia 2) and neuroblastoma cells in vitro 4) and that it triggers apoptotic neurodegeneration in the developing rat brain in vivo.…”

“…5) It has been reported that alcohol induces death in a variety of cells including astroglia 2) and neuroblastoma cells in vitro 4) and that it triggers apoptotic neurodegeneration in the developing rat brain in vivo. 3) In addition, alcohol intake during the developmental stage has been associated with deficits in learning and memory, 6,7) and alcohol abuse has been shown to induce behavioral disorders during adolescence.…”