Ethanol Improves Decreased Filterability of Human Red Blood Cells Through Modulation of Intracellular Signaling Pathways

Oonishi, Tadahiro; Sakashita, Kanako

doi:10.1111/j.1530-0277.2000.tb04621.x

Cited by 11 publications

(8 citation statements)

References 18 publications

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“…The mean values of sphericity were 0.65 ± 0.04, 0.65 ± 0.04, 0.67 ± 0.05, and 0.65 ± 0.05 for RBCs exposed to the 0.0, 0.1, 0.3, and 0.5% ethanol concentrations, respectively. In both cases, the observed recoveries of both mean surface area and sphericity of RBCs in the 0.5% ethanol group to the normal ranges of healthy RBCs might be not only related to certain abrupt changes in intracellular mechanisms at a lethal alcoholic condition, but also consistent with a previous report about biphasic property of RBC deformability in relevant ethanol concentrations [ 14 ]. Values of the measured cell surface area, and sphericity are in good agreement with precedent studies employing micropipette aspiration [ 28 ], osmotic feasibility [ 29 ], and flow-based imaging [ 30 ].…”

Section: Resultssupporting

confidence: 91%

“…Interestingly, exactly this ethanol level, i.e. 0.5%, has been reported as a phase transition point where the filterability of RBCs through micro-size pores started to decrease [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

“…Alcohol-induced modifications in red blood cell (RBC) membranes have been extensively investigated as a model system using diverse experimental techniques that include EPR spectroscopy [ 1 , 5 , 6 ], fluorescence anisotropy [ 7 – 11 ], gas chromatography [ 12 ], laser diffraction ektacytometry [ 6 , 13 ], and micro-size mesh filtration [ 14 ]. The ethanol-mediated modifications in hemorheological parameters of RBCs, including mean corpuscular hemoglobin (Hb) concentration (MCHC) and mean corpuscular volume (MCV), were measured with the help of flow cytometry for in-vitro ethanol exposures [ 15 ] and in-vivo ethanol ingestions [ 15 ], and from RBCs of chronic alcoholics [ 9 – 11 ].…”

Section: Introductionmentioning

confidence: 99%

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The Effects of Ethanol on the Morphological and Biochemical Properties of Individual Human Red Blood Cells

et al. 2015

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Here, we report the results of a study on the effects of ethanol exposure on human red blood cells (RBCs) using quantitative phase imaging techniques at the level of individual cells. Three-dimensional refractive index tomograms and dynamic membrane fluctuations of RBCs were measured using common-path diffraction optical tomography, from which morphological (volume, surface area, and sphericity); biochemical (hemoglobin (Hb) concentration and Hb content); and biomechanical (membrane fluctuation) parameters were retrieved at various concentrations of ethanol. RBCs exposed to the ethanol concentration of 0.1 and 0.3% v/v exhibited cell sphericities higher than those of normal cells. However, mean surface area and sphericity of RBCs in a lethal alcoholic condition (0.5% v/v) are not statistically different with those of healthy RBCs. Meanwhile, significant decreases of Hb content and concentration in RBC cytoplasm at the lethal condition were observed. Furthermore, dynamic fluctuation of RBC membranes increased significantly upon ethanol treatments, indicating ethanol-induced membrane fluidization.

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Section: Resultssupporting

confidence: 91%

“…Interestingly, exactly this ethanol level, i.e. 0.5%, has been reported as a phase transition point where the filterability of RBCs through micro-size pores started to decrease [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Effects of Ethanol on the Morphological and Biochemical Properties of Individual Human Red Blood Cells

et al. 2015

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“…3B-C) are important for modulation of erythrocyte membrane stability and deformability [148]. Interestingly, activation of protein kinase C [149] and cAMP-dependent protein kinase [150] partially improve RBC filterability.…”

Section: +mentioning

confidence: 99%

Erythrocyte Signal Transduction Pathways, their Oxygenation Dependence and Functional Significance

Barvitenko

Adragna

Weber³

2005

Cell Physiol Biochem

135

104

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Erythrocytes play a key role in human and vertebrate metabolism. Tissue O₂ supply is regulated by both hemoglobin (Hb)-O₂ affinity and erythrocyte rheology, a key determinant of tissue perfusion. Oxygenation-deoxygenation transitions of Hb may lead to re-organization of the cytoskeleton and signalling pathways activation/deactivation in an O₂-dependent manner. Deoxygenated Hb binds to the cytoplasmic domain of the anion exchanger band 3, which is anchored to the cytoskeleton, and is considered a major mechanism underlying the oxygenation-dependence of several erythrocyte functions. This work discusses the multiple modes of Hb-cytoskeleton interactions. In addition, it reviews the effects of Mg²⁺, 2,3-diphosphoglycerate, NO, shear stress and Ca²⁺, all factors accompanying the oxygenation-deoxygenation cycle in circulating red cells. Due to the extensive literature on the subject, the data discussed here, pertain mainly to human erythrocytes whose O₂ affinity is modulated by 2,3-diphosphoglycerate, ectothermic vertebrate erythrocytes that use ATP, and to bird erythrocytes that use inositol pentaphosphate.

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“…Alcohol influences blood velocity. The physiological and pharmacological actions of ethanol are influenced by molecules penetrating into the membrane, which leads to changes in shape, function, and interactions of the membrane proteins [15]. The proposed model could shed light on the issue of how the different factors influence RBC behavior in a microvessel.…”

Section: Resultsmentioning

confidence: 99%

Numerical Study of RBC Motion and Deformation through Microcapillary in Alcohol Plasma Solution

Ni¹,

Cheema²,

Park³

2015

OJFD

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Alcohol influences human health condition by starving red blood cells (RBCs) of oxygen, which results in poor blood circulation. Starved RBCs clump together and restrict blood flow, especially in capillaries. In this study, a finite element method-based moving mesh technique was applied to simulate the motion and deformation of a single RBC under different flow conditions. A 2-D model of a single RBC floating in plasma-alcohol solution was created using Arbitrary Lagrangian-Eulerian (ALE) method with moving mesh for a fluid structure interaction problem. Cell deformability and stability were studied in an alcoholic plasma solution at different fluid flow conditions. Poor blood circulation was observed with RBC tending to rotate and oscillate at low flow rates. Moreover, RBC exhibited a parachute shape while moving without oscillation, which indicated improved micro-circulation at increased flow rates. In both cases, RBC exhibited a parachute shape while moving through micro-channel at increased flow rates. The simulation also showed the significant increase of RBC deformability with the increasing viscosity of plasma as a result of alcohol presence in blood.

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Ethanol Improves Decreased Filterability of Human Red Blood Cells Through Modulation of Intracellular Signaling Pathways

Abstract: The present results suggest a beneficial effect of ethanol on blood rheology at concentrations that are physiologically achievable in the blood and indicate the importance of intracellular signaling pathways in regulating the deformability of RBCs.

Cited by 11 publications

References 18 publications

The Effects of Ethanol on the Morphological and Biochemical Properties of Individual Human Red Blood Cells

The Effects of Ethanol on the Morphological and Biochemical Properties of Individual Human Red Blood Cells

Erythrocyte Signal Transduction Pathways, their Oxygenation Dependence and Functional Significance

Numerical Study of RBC Motion and Deformation through Microcapillary in Alcohol Plasma Solution

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