Ethanol fermentation by the thermotolerant yeast, &lt;em&gt;Kluyveromyces marxianus &lt;/em&gt;TISTR5925, of extracted sap from old oil palm trunk

Murata, Yoshinori; Danjarean, Hatairat; Fujimoto, Kiyohiko; Kosugi, Akihiko; Arai, T.; Ibrahim, Wan Asma; Suliman, Othman; Hashim, Rokiah; Mori, Yutaka

doi:10.3934/energy.2015.2.201

Cited by 14 publications

(10 citation statements)

References 17 publications

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“…2 Growth performance of thermotolerant yeast isolates at 37, 40, 42, and 45°C after 48 h of incubation time on YPD plates acetic acid, glycerol, trehalose, and succinic acid (Singer and Lindquist 1998). The use of thermotolerant yeasts during ethanol production is beneficial because they do not require overall costs necessary for thermal maintenance and distillation (Fonseca et al 2008), additions of the exogenous enzyme (Abdel-Banat et al 2010), and have less chance of bacterial contamination (Murata et al 2015).…”

Section: Screening Of Yeast Isolates For Stress Tolerancementioning

confidence: 99%

Isolation and characterization of bioethanol producing wild yeasts from bio-wastes and co-products of sugar factories

et al. 2022

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Purpose Yeasts are widely used for the production of bioethanol from biomasses rich in sugar. The present study was aimed at isolating, screening, and characterizing fermentative wild yeast recovered from bio-waste and co-products of Ethiopian sugar factories for bioethanol production using sugarcane molasses as a substrate. Method The wild yeasts were identified according to their cellular morphology and D1/D2 and ITS1-5.8S-ITS2 rDNA sequencing. Analysis of ethanol and by-product concentration was done by HPLC equipped with a UV detector. Higher alcohols, acetaldehyde, and methanol were analyzed using GC-MS equipped with a flame ionization detector (FID). Result Seven strains (Meyerozyma caribbica MJTm3, Meyerozyma caribbica MJTPm4, Meyerozyma caribbica SHJF, Saccharomyces cerevisiae TA2, Wickerhamomyces anomalus MJTPm2, Wickerhamomyces anomalus 4m10, and Wickerhamomyces anomalus HCJ2F) were found tolerant to 18% (v/v) ethanol, whereas one strain Meyerozyma caribbica MJTm3 tolerated 20%. These strains also showed tolerance to 45°C, 50% of sugar, and pH 2–10. Meyerozyma caribbica MJTm3 produced 12.7% (v/v) of alcohol with an actual ethanol concentration of 26 g L−1, an ethanol yield of 47%, 78% of theoretical yield, and a productivity of 0.54 g L−1 h−1 from 30 °Brix of molasses at 48 h incubation under laboratory scale. Based on the one variable at a time optimization (OVAT), the optimal parameters for maximum bioethanol production were at initial pH 5.5, 35 °Brix, 30°C, 15% inoculum size, 150 rpm, 4 g L−1 di-ammonium phosphate supplement, and 48 h incubation. Under these optimum conditions, 14% (v/v) alcohol, 42 g L−1 actual ethanol concentration, 69% ethanol yield, 89% of theoretical yield, and productivity of 0.88 g L−1 h−1 were obtained. Conclusion These results indicated that M. caribbica MJTm3 should further be evaluated, optimized, and improved for industrial bioethanol production due to its fermentation potential.

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Section: Screening Of Yeast Isolates For Stress Tolerancementioning

confidence: 99%

Isolation and characterization of bioethanol producing wild yeasts from bio-wastes and co-products of sugar factories

et al. 2022

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“…Several researchers have isolated thermotolerant yeast strains that play a role in the bioconversion of lignocellulosic biomass into value-added products (Tanimura et al 2012 ; Murata et al 2015 ; Ndubuisi et al 2018 ; Pongcharoen et al 2018 ; Phong et al 2019 ; Ulya et al 2018 ; Rahman et al 2021 ; Pilap et al 2022 ). Pichia kudriavzevii is one of the most widely isolated thermotolerant yeast species in these studies, which can ferment sugar into ethanol (Ndubuisi et al 2018 ; Pongcharoen et al 2018 ; Ulya et al 2018 ; Rahman et al 2021 ; Pilap et al 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Isolation of xylose-utilizing yeasts from oil palm waste for xylitol and ethanol production

Kusumawati,

Sumarlan,

Zubaidah

et al. 2023

Bioresour. Bioprocess.

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The energy crisis triggers the use of energy sources that are renewable, such as biomass made from lignocellulosic materials, to produce various chemical compounds for food ingredients and biofuel. The efficient conversion of lignocellulosic biomass into products with added value involves the activity of microorganisms, such as yeasts. For the conversion, microorganisms must be able to use various sugars in lignocellulosic biomass, including pentose sugars, especially xylose. This study aims to isolate xylose-utilizing yeasts and analyze their fermentation activity to produce xylitol and ethanol, as well as their ability to grow in liquid hydrolysate produced from pretreated lignocellulosic biomass. Nineteen yeast isolates could grow on solid and liquid media using solely xylose as a carbon source. All isolates can grow in a xylose medium with incubation at 30 °C, 37 °C, 42 °C, and 45 °C. Six isolates, namely SLI (1), SL3, SL6, SL7, R5, and OPT4B, were chosen based on their considerable growth and high xylose consumption rate in a medium with 50 g/L xylose with incubation at 30 °C for 48 h. Four isolates tested, namely SLI (1), SL6, SL7, and R5, can produce xylitol in media containing xylose carbon sources. The concentration of xylitol produced was determined using high-pressure liquid chromatography (HPLC), and the results ranged from 5.0 to 6.0 g/L. Five isolates tested, namely SLI (1), SL6, SL3, R5, and OPT4B, can produce ethanol. The ethanol content produced was determined using gas chromatography (GC), with concentrations ranging from 0.85 to 1.34 g/L. Three isolates, namely SL1(1), R5, and SL6, were able to produce xylitol and ethanol from xylose as carbon sources and were also able to grow on liquid hydrolyzate from pretreated oil palm trunk waste with the subcritical water method. The three isolates were further analyzed using the 18S rDNA sequence to identify the species and confirm their phylogenetic position. Identification based on DNA sequence analysis revealed that isolates SL1(1) and R5 were Pichia kudriavzevii, while isolate SL6 was Candida xylopsoci. The yeast strains isolated from this study could potentially be used for the bioconversion process of lignocellulosic biomass waste to produce value-added derivative products. Graphical Abstract

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“…Reed (1982) and Sedha and Verma (2002) have also recommended 30°C as optimal temperature for ethanol production. Most of the yeasts capable of producing ethanol are not thermotolerant and their growth and fermentation efficiency decreases at 40°C (Murata et al 2015;Antil et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Saccharification of biopretreated paddy straw with indigenous holocellulase and fermentation with Saccharomyces cerevisiae LN1 under optimized conditions

Priya

Tiwari

Rana

et al. 2016

Energ. Ecol. Environ.

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The current study was aimed at optimizing the fermentation conditions for efficient ethanol production from biologically pretreated paddy straw. The yeast strain Saccharomyces cerevisiae LN1 showed highest fermentation efficiency at pH 5.0 and temperature 30°C. Paddy straw pretreated with fungus Myrothecium roridum LG7 was saccharified with indigenous holocellulase from Aspergillus niger SH3 producing total sugar yield of 26.14 mg/ml with 19.23 mg/ml of glucose. Enzymatic hydrolysate was then fermented using S. cerevisiae LN1 to observe the effect of nutrient supplementation (yeast extract, MgSO 4 Á7H 2 O and (NH 4 ) 2 SO 4 ) on ethanol production. Higher ethanol was produced from saccharified material fermented without supplementation of any nutrient source. With the scale-up of ethanol production under optimized conditions in 7L bioreactor, 4.46 g/l of ethanol was produced with fermentation efficiency of 47.2 %. TLC of enzymatic hydrolysate confirmed the presence of p-coumaric acid, ferulic acid, vanillic acid, gallic acid and many other aromatic compounds and inhibitors in the saccharified material which limit fermentation efficiency of yeast strain. Thus, optimization of fermentation conditions can lead to development of a cost-effective process for efficient ethanol production, exploitation of which also requires removal of aromatic compounds and inhibitors which may hinder the ethanol production efficiency.

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Ethanol fermentation by the thermotolerant yeast, <em>Kluyveromyces marxianus </em>TISTR5925, of extracted sap from old oil palm trunk

Cited by 14 publications

References 17 publications

Isolation and characterization of bioethanol producing wild yeasts from bio-wastes and co-products of sugar factories

Isolation and characterization of bioethanol producing wild yeasts from bio-wastes and co-products of sugar factories

Isolation of xylose-utilizing yeasts from oil palm waste for xylitol and ethanol production

Saccharification of biopretreated paddy straw with indigenous holocellulase and fermentation with Saccharomyces cerevisiae LN1 under optimized conditions

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