Ethanol extracts of Sanguisorba officinalis L. suppress TNF-α/IFN-γ-induced pro-inflammatory chemokine production in HaCaT cells

Yang, Ju-Hye; Hwang, Youn–Hwan; Gu, Min-Jung; Cho, Won‐Kyung; Yeul, Jin

doi:10.1016/j.phymed.2015.09.006

Cited by 61 publications

(48 citation statements)

References 23 publications

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“…Sanguisorbae Radix has been reported that it possesses some beneficial effects such as anti-cancer [13], anti-inflammation [5, 14], anti-oxidation [15], neuroprotection [16, 17] and renal protection [18]. Currently, we have reported the inhibitory action of its ethanol extract on 2, 4-dinitrochlorobenzene (DNCB)-induced AD model [19].…”

Section: Discussionmentioning

confidence: 99%

“…For measurements of TARC, RANTES, MDC, IL-8 and β-actin mRNA, the PCR conditions were as follows: 12 cycles of primer annealing at 25 °C for 30 s, cDNA synthesis at 45 °C for 4 min, melting of the secondary structure and cDNA synthesis at 55 °C for 30 s, and heat inactivation at 95 °C for 5 min. The PCR-amplified primers used in this study are described in previously described [5]. Gene expression was quantified by real-time PCR using the AccuPower 2× Greenstar qPCR Master (Bioneer) according to the following protocol: pre-denaturation at 95 °C for 10 min, followed by 40 cycles of 95 °C for 10 s, 60 °C for 30 s, and 72 °C for 30 s. Amplifications were carried out using QuantStudio 6 (LifeScience, ABI, USA), The fold change in the expression of the target gene relative to the control was normalized to β-actin using the 2 -ΔΔCt method.…”

Section: Methodsmentioning

confidence: 99%

“…Stimulation of keratinocytes with TNF-α and IFN-γ induces the expression of pro-inflammatory chemokines including thymus and activation regulated chemokine (TARC; CCL17), regulated on activation, normal T-cell expressed and secreted (RANTES; CCL5), macrophage-derived chemokine (MDC; CCL22) and interleukin 8 (IL-8; CXCL8) [4, 5]. …”

Section: Introductionmentioning

confidence: 99%

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Anti-inflammatory effects of Sanguisorbae Radix water extract on the suppression of mast cell degranulation and STAT-1/Jak-2 activation in BMMCs and HaCaT keratinocytes

Yang

Yoo

Cho

et al. 2016

BMC Complement Altern Med

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BackgroundSanguisorbae Radix (SR) is a well-known herbal medicine used to treat inflammatory disease and skin burns in Asia. In addition, it is used to treat many types of allergic skin diseases, including urticaria, eczema, and allergic dermatitis. SR has been reported to exhibit anti-wrinkle, anti-oxidant, and anti-contact dermatitis bioactivities.MethodsIn this study, we investigated the mechanism underlying the anti-inflammatory effects of SR water extract (WSR) using human keratinocyte (HaCaT) cells and BALB/c mouse bone marrow-derived mast cells (BMMCs). Viability assays were used to evaluate non-cytotoxic concentrations of WSR in both BMMCs and HaCaT cells. To investigate the effect of WSR treatment on the degranulation of IgE/Ag-activated BMMCs, we measured the release of β-hexosaminidase (β-HEX). We determined the production of pro-inflammatory chemokines including thymus and activation regulated chemokine (TARC; CCL17), regulated on activation, normal T-cell expressed and secreted (RANTES; CCL5), macrophage-derived chemokine (MDC; CCL22), and interleukin 8 (IL-8; CXCL8) in stimulated human keratinocytes. The ability of WSR to reduce the expression of pro-inflammatory marker proteins was evaluated by Western blotting in HaCaT cells stimulated with tumor necrosis factor (TNF)-α/interferon (IFN)-γ.ResultWSR inhibited IgE/Ag-activated mast cell degranulation in BMMCs. Treatment with various concentrations of WSR decreased β-HEX release in a dose-dependent manner with an IC50 of 27.5 μg/mL. In keratinocytes, WSR suppressed TNF-α/IFN-γ-induced chemokine production and pro-inflammatory molecules via a blockade STAT-1, Jak-2, p38, and JNK activation.ConclusionsThis results demonstrate that WSR inhibits degranulation of IgE/Ag-activated mast cells and inhibits the production of pro-inflammatory chemokines by suppressing the phosphorylation of p38 and JNK in HaCaT cells.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Anti-inflammatory effects of Sanguisorbae Radix water extract on the suppression of mast cell degranulation and STAT-1/Jak-2 activation in BMMCs and HaCaT keratinocytes

Yang

Yoo

Cho

et al. 2016

BMC Complement Altern Med

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“…have isolated antioxidants such as fisetinidol‐(4α‐8)‐catechin and (+)‐catechin from ethanol extracts . Another study has found that ethanol extracts suppress production of TNF‐α/IFN‐γ‐induced pro‐inflammatory chemokines and their representative components, such as (+)‐catechin, (−)‐epicatechin, and ziyuglycoside I . However, the active compounds of HSO and their possible physiological functions are still not understood.…”

Section: Resultsmentioning

confidence: 99%

A hot‐water extract of Sanguisorba officinalis ameliorates endotoxin‐induced septic shock by inhibiting inflammasome activation

Seo

Cho

et al. 2018

Microbiology and Immunology

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The inflammasome is a multiprotein signaling complex that mediates inflammatory innate immune responses through caspase 1 activation and subsequent IL-1b secretion. However, because its aberrant activation often leads to inflammatory diseases, targeting the inflammasome holds promise for the treatment of inflammation-related diseases. In this study, it was found that a hot-water extract of Sanguisorba officinalis (HSO) suppresses inflammasome activation triggered by adenosine 5 0 -triphosphate, nigericin, microbial pathogens, and double stranded DNA in bone marrow-derived macrophages. HSO was found to significantly suppress IL-1b production in a dose-dependent manner; this effect correlated well with small amounts of caspase 1 and little ASC pyroptosome formation in HSO-treated cells. The anti-inflammatory activity of HSO was further confirmed in a mouse model of endotoxin-induced septic shock. Oral administration of HSO reduced IL-1b titers in the serum and peritoneal cavity, increasing the survival rate. Taken together, our results suggest that HSO is an inhibits inflammasome activation through nucleotide-binding domain and leucine-rich repeat pyrin domain 3, nucleotide-binding domain and leucine-rich repeat caspase recruitment domain 4 and absent in melanoma 2 pathways, and may be useful for treatment of inflammasomemediated diseases.Key words endotoxin-induced septic shock, hot-water extract, inflammasome, Sanguisorba officinalis.Sanguisorba officinalis is a member of the family Rosaceae. The root of this plant, called "Jiyu" in Korea, is often used in traditional oriental medicine to treat burns, scalds, diarrhea and internal hemorrhage (1). Various other beneficial effects of S. officinalis have recently been reported, including anti-allergic, antiwrinkle, anti-cancer, anti-oxidant, hemostatic and immunomodulatory activities (2-8). In a recent study, significantly greater amounts of anti-oxidant compounds-phenolics and flavonoids-were found in both water and ethanol extracts of S. officinalis than in other Chinese medicinal plants (9). An ethanolic extract of S. officinalis has been shown to exert anti-inflammatory and anti-asthmatic effects, these being mediated by nuclear factor-kappa B and activator protein 1 suppression and heme oxygenase-1 induction, respectively (10, 11). An aqueous extract of S. officinalis also reportedly shows anti-inflammatory activity with less toxicity (9), as

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“…The components in the CSF formula have various effects, including anti-inflammation, antibacterial activity, blood nourishment, pain relief, hemostasis, etc. (16)(17)(18)(19)(20)(21)(22)(23)(24). CSF has demonstrated improvement in local microcirculation, control of local inflammation, pain relief, and promotion of wound healing.…”

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confidence: 99%

Enhancement of Wound Healing by the Traditional Chinese Medicine Herbal Mixture Sophora flavescens in a Rat Model of Perianal Ulceration

Zhang

et al. 2017

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Ethanol extracts of Sanguisorba officinalis L. suppress TNF-α/IFN-γ-induced pro-inflammatory chemokine production in HaCaT cells

Cited by 61 publications

References 23 publications

Anti-inflammatory effects of Sanguisorbae Radix water extract on the suppression of mast cell degranulation and STAT-1/Jak-2 activation in BMMCs and HaCaT keratinocytes

Anti-inflammatory effects of Sanguisorbae Radix water extract on the suppression of mast cell degranulation and STAT-1/Jak-2 activation in BMMCs and HaCaT keratinocytes

A hot‐water extract of Sanguisorba officinalis ameliorates endotoxin‐induced septic shock by inhibiting inflammasome activation

Enhancement of Wound Healing by the Traditional Chinese Medicine Herbal Mixture Sophora flavescens in a Rat Model of Perianal Ulceration

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