Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Standardized Ameliorates Hyperglycemia, Polyphagia, and Weight Loss in Diabetic Rats

Fauziyah, Yulia; Sunarti, Sunarti; Hanoum, Ita Fauzia; Wahyuningsih, Mae Sri Hartati

doi:10.20884/1.jm.2018.13.1.417

Cited by 7 publications

(6 citation statements)

References 16 publications

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“…10 Tithonia diversifolia was demonstrated to reduce the blood sugar levels of diabetic rats. 11,12 In a previous study, TDL extract at doses of 25 mg/kg BW, 50 mg/kg BW, and 100 mg/kg BW significantly reduce blood sugar levels.12 However, in this study, with a similar treatment, TDL extract administration at a 25 mg/kg BW dose was insufficient to increase the NO level, as shown in Table 1. Meanwhile, K.4 and K.5 groups demonstrated an increase in NO levels compared to the diabetic control group.…”

Section: Discussionmentioning

confidence: 54%

The effect of Tithonia diversifolia extract against the level of nitric oxide in streptozotocin-nicotinamide-induced rats model

Sati,

Rahmadianto,

Kuncoro

et al. 2022

JKKI

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Background: One indication of microvascular dysfunction in diabetes mellitus (DM) is decreased nitric oxide (NO) levels. Tithonia diversifolia leaves (TDL) extract has been scientifically demonstrated to decrease glucose levels in diabetic rats.Objective: This study aims to examine the effect of TDL extract on NO levels in diabetic rats.Methods: True experimental with pre-post test control group design used 25 Sprague-Dawley rats. Its were divided into 5 groups: healthy control (K.1); diabetic control (K.2); diabetes rats with ethanol extract of TDL at a dose of 25 (K.3); 50 (K.4); and 100 mg/kg BW (K.5) for 28 days. We use a combination of nicotinamide (230 mg/kg BW) and streptozotocin (65 mg/kg BW) to induce diabetes mellitus. The blood serum was taken before and after extract administration. NO level was assessed using spectrophotometry. Paired T-test, Wilcoxon, one-way ANOVA, and post hoc LSD were performed for statistical analysis.Results: There were significant differences in NO levels before and after treatment in all groups unless K.5 (100 mg/kg BW). Furthermore, there was no significant difference in NO levels in the healthy control and the K.5 group but significant differences in other groups.Conclusion: TDL extract can prevent the decrease in NO level in diabetic rats model with the effective dose of 100 mg/kg BW.

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Section: Discussionmentioning

confidence: 54%

The effect of Tithonia diversifolia extract against the level of nitric oxide in streptozotocin-nicotinamide-induced rats model

Sati,

Rahmadianto,

Kuncoro

et al. 2022

JKKI

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“…Polyphagia results from cells experiencing a lack of energy because glucoe cannot be absorbed into the cell. Fauziah et al (2018) explained when the cells deficient glucose will activate the hunger center located in the hypothalamus section so increase food intake. When the cells in the condition lack of energy, adipose is presumably because the dose used in the study is lower.…”

Section: Results and Disscusionsmentioning

confidence: 99%

“…Sesquiterpen lactone compounds and tagitinin A can increase peroxisome proliferator-activated recep-tors (PPARs) ϒ activity so as to increase insulin sensitivity (Lin, 2012). PPARs ϒ activation can increased the expression of a number of genes associated with glucose metabolism and regulated insulin transcription in pancreatic β cells (Fauziah et al, 2018). Increasing body weight in glibenclamide treatments was thought to be a side effect of the ability to reduce blood glucose levels.…”

Section: Results and Disscusionsmentioning

confidence: 99%

Antihyperglycemic Activity of Aqueous Extract of Insulin Leaves (Tithonia diversifolia) on Hyperglycemic Rats (Rattus norvegicus)

2018

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Insulin leave (Tithonia diversifolia (Hemsl.) A. Gray) empirically used by people as antihyperglycemic drugs. The study was aimed to evaluate antihyperglycemic activity as well as to determine the most optimum dose of T. diversifolia to reduce blood glucose levels. Hyperglycemic condition was induced to male Rattus norvegicus rats by intraperitoneal injection of alloxan at a dose of 150 mg/kg bw (body weight). In this study, completely randomized design was performed with three treatment groups and five times repetition. Group P1 received glibenclamide 10 mg/kg bw, P2 received aqueous extract of T. diversifolia at a dose of 150 mg/kg bw, P3 received aqueous extract of T. diversifolia at a dose of 300 mg/kg bw. Administration of both glibenclamide and aqueous extract of T. diversifolia was conducted orally for 28 days. Data were analyzed using Anova and Duncan’s test with 95% confidence level (α = 0.05). The results showed that the mean percentage of decrease in blood glucose levels, drink intake and body weight of all treatment groups were not significantly different (p>0.05). Data of feed intake showed that P1 was significantly different from P2 (p<0.05), but P2 was not significantly different from P3 (p>0.05). Based on the result of this research, it was found that the low doses of aqueous extract of T. diversifolia has the same ability to decrease blood glucose level compared to glibenclamide. Futhermore, this study provide some information that can be used as preclinical analysis to determine effective doses of aqueous extract of T. diversifolia to decrease blood glucose levels.

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“…In our previous study, T. diversifolia extract was shown to exert anti-hyperglycaemic effects ( 6 ). Therefore, in the present study, we improved the quality and reproducibility of the extract through a more complete standardisation process that included several specific and non-specific parameters.…”

Section: Discussionmentioning

confidence: 99%

“…Tithonia diversifolia (Hemsley) A Gray extract antihyperglycaemic activity that is beneficial for the treatment of T2DM ( 6 ). In vitro studies have demonstrated the ability of T. diversifolia to act as a peroxisome proliferator activator receptor (PPARα/γ) agonist ( 7 ), which increases 5’AMP-activated protein kinase (AMPK) phosphorylation ( 8 ).…”

Section: Introductionmentioning

confidence: 99%

Standardised Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Leaves Improve Insulin Sensitivity and Increase Mitochondrial DNA Copy Numbers in Skeletal Muscles of Streptozotocin-Nicotinamide-Induced Rats

Istikharah

Nugrahaningsih

Sadewa

et al. 2022

MJMS

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Background: In this study, we examined the anti-diabetic activity of standardised extracts of Tithonia diversifolia (Hemsley) A Gray (T. diversifolia) leaves for their effects on insulin resistance and mitochondrial DNA (mtDNA) copy number. Methods: T. diversifolia leaves were extracted using an ultrasound-assisted method and standardised using Tagitinin C. There were six groups: i) normal control; ii) diabetic group; iii) metformin group (300 mg/kg) and iv) groups treated with three different doses of extract (50 mg/kg, 100 mg/kg and 150 mg/kg). Blood samples were taken before and after 28 days of treatment for fasting plasma glucose (FPG) and insulin analysis, which were used for a Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) calculation. The soleus and gastrocnemius muscles were harvested after 28 days of treatment for the measurement of mtDNA copy number. Results: The results showed an improvement in blood glucose levels and HOMA-IR scores in all treatment groups. The results of mtDNA copy number analysis also revealed significant improvement with the highest number observed at an extract dose of 100 mg/kg in which the mtDNA copy number increased up to 3 times in the soleus muscles (P < 0.001). Conclusion: T. diversifolia extract has the potential to be used as an anti-diabetic agent that improves insulin resistance, possibly by increasing mtDNA content.

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Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Standardized Ameliorates Hyperglycemia, Polyphagia, and Weight Loss in Diabetic Rats

Cited by 7 publications

References 16 publications

The effect of Tithonia diversifolia extract against the level of nitric oxide in streptozotocin-nicotinamide-induced rats model

The effect of Tithonia diversifolia extract against the level of nitric oxide in streptozotocin-nicotinamide-induced rats model

Antihyperglycemic Activity of Aqueous Extract of Insulin Leaves (Tithonia diversifolia) on Hyperglycemic Rats (Rattus norvegicus)

Standardised Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Leaves Improve Insulin Sensitivity and Increase Mitochondrial DNA Copy Numbers in Skeletal Muscles of Streptozotocin-Nicotinamide-Induced Rats

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