Ethanol extract of Dryopteris crassirhizoma alleviates allergic inflammation via inhibition of Th2 response and mast cell activation in a murine model of allergic rhinitis

Piao, Chun Hua; Kim, Tae-Geum; Bui, Thi; Song, Chang Ho; Shin, Dong Uk; Eom, Ji-Eun; Lee, So‐Young; Shin, Hee Soon; Chai, Ok Hee

doi:10.1016/j.jep.2018.11.037

Cited by 17 publications

(10 citation statements)

References 47 publications

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“…4,5,6), suggesting that the aqueous extract of BLAB may upregulate Th1 and Treg immune response. In addition, the levels of histamine one of the most potent vasoactive mediators implicated in the acute phase of immediate hypersensitivity [7,35], which were decreased in the BLAB group compared to those in the OVA group (Fig. 5A).…”

Section: Discussionmentioning

confidence: 97%

“…Male mice were randomly divided into six groups (n = 12 per group), as follows: control, OVA, BLAB100, BLAB200, BLAB400, and Dex groups. The OVA-induced AR mouse model and treatment were established according to Piao et al [7,8] (Fig. 1).…”

Section: Establishment Of Allergic Rhinitis Model and Treatmentmentioning

confidence: 99%

“…Flow cytometry and the Human Regulatory T cell Staining Kit (eBioscience, USA) for Treg cell were used according to the manufacturer's protocols [7]. The serum levels of total IgE, anti-OVA specific IgE, IgG1, and IgG2a were measured using ELISA kits (Invitrogen, San Diego, CA, USA) and serum levels of histamine were quantified using the histamine assay kit (R&D Systems Inc, USA) [8], following the manufacturers' instructions.…”

Section: Measurements Of Treg Cells Cytokines and Histaminementioning

confidence: 99%

“…In addition, regulatory T (Treg) cells, which are known to inhibit the proliferation and activation of conventional effector T cells, have been found to play an important role in maintaining immune homeostasis [6]. Therefore, it can be hypothesized that regulation of the Th1/Th2/Treg immune balance may be an effective treatment strategy for AR and other allergic diseases [7].…”

Section: Introductionmentioning

confidence: 99%

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The extract of black cumin, licorice, anise, and black tea alleviates OVA-induced allergic rhinitis in mouse via balancing activity of helper T cells in lung

Liao¹,

Han²,

Chen³

et al. 2021

Allergy Asthma Clin Immunol

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Background A formulation of black cumin (Nigella sativa L.), licorice (Glycyrrhiza glabra L.), anise (Pimpinella anisum L.) and tea (Camellia sinensis (L.) Kuntze) (denoted BLAB tea) is traditionally used to relief allergy reaction including allergic rhinitis. However, little is known about its underlining mechanism of anti-allergic effects. Methods To investigate the anti-allergenic mechanism of BLAB tea, we treated ovalbumin (OVA)-induced allergic rhinitis (AR) model of mice with BLAB tea, and elucidated its possible mechanism of action. Mice in the control group were treated with phosphate-buffered saline only. Subsequently, the infiltration of different inflammatory cells was measured. In addition, histopathological changes in the nasal mucosa, and the levels of allergen-specific cytokines and OVA-specific immunoglobulins were measured. Results The aqueous extract of BLAB significantly alleviated the nasal symptoms and reduced the accumulation of inflammatory cells in the nasal mucosa and nasal lavage fluid of AR model of mice. Conclusion The aqueous extract of BLAB induced the production of Th1 and Treg cytokines and inhibited the release of Th2 cytokines and histamine in nasal mucosa and serum of mice while decreasing the serum levels of OVA-specific IgE, IgG1, and IgG2a. These results suggest the potential of the aqueous extract of BLAB as a treatment option for allergic diseases.

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Section: Discussionmentioning

confidence: 97%

Section: Establishment Of Allergic Rhinitis Model and Treatmentmentioning

confidence: 99%

Section: Measurements Of Treg Cells Cytokines and Histaminementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The extract of black cumin, licorice, anise, and black tea alleviates OVA-induced allergic rhinitis in mouse via balancing activity of helper T cells in lung

Liao¹,

Han²,

Chen³

et al. 2021

Allergy Asthma Clin Immunol

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“…Reportedly, the IgG1 immune complex is responsible for class-switching to Th2 cytokines, and IgG2a is oriented toward Th1 cytokine immune deviation [36]. In addition, adjusting the balance of Th1/Th2 cytokines to inhibit mast cell activation, then decreasing the levels of IgG1 and increasing IgG2a has a significant anti-allergic inflammatory effect [37]. We found that topical treatment with SP reduced IgE levels and regulated IgG1 and IgG2a levels in DNCB-treated BALB/c mice (Figure 3E).…”

Section: Discussionmentioning

confidence: 99%

Topical Spilanthol Inhibits MAPK Signaling and Ameliorates Allergic Inflammation in DNCB-Induced Atopic Dermatitis in Mice

Huang

et al. 2019

IJMS

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Atopic dermatitis (AD) is a recurrent allergic skin disease caused by genetic and environmental factors. Patients with AD may experience immune imbalance, increased levels of mast cells, immunoglobulin (Ig) E and pro-inflammatory factors (Cyclooxygenase, COX-2 and inducible NO synthase, iNOS). While spilanthol (SP) has anti-inflammatory and analgesic activities, its effect on AD remains to be explored. To develop a new means of SP, inflammation-related symptoms of AD were alleviated, and 2,4-dinitrochlorobenzene (DNCB) was used to induce AD-like skin lesions in BALB/c mice. Histopathological analysis was used to examine mast cells and eosinophils infiltration in AD-like skin lesions. The levels of IgE, IgG1 and IgG2a were measured by enzyme-linked immunosorbent assay (ELISA) kits. Western blot was used for analysis of the mitogen-activated protein kinase (MAPK) pathways and COX-2 and iNOS protein expression. Topical SP treatment reduced serum IgE and IgG2a levels and suppressed COX-2 and iNOS expression via blocked mitogen-activated protein kinase (MAPK) pathways in DNCB-induced AD-like lesions. Histopathological examination revealed that SP reduced epidermal thickness and collagen accumulation and inhibited mast cells and eosinophils infiltration into the AD-like lesions skin. These results indicate that SP may protect against AD skin lesions through inhibited MAPK signaling pathways and may diminish the infiltration of inflammatory cells to block allergic inflammation.

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Mitochondrial dynamics modulate the allergic inflammation in a murine model of allergic rhinitis

Chen,

Zhou,

et al. 2023

Immunity Inflam & Disease

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ObjectiveAllergic rhinitis (AR) is a common allergic disorder, afflicting thousands of human beings. Aberrant mitochondrial dynamics are important pathological elements for various immune cell dysfunctions and allergic diseases. However, the connection between mitochondrial dynamics and AR remains poorly understood. This study aimed to determine whether mitochondrial dynamics influence the inflammatory response in AR.MethodsIn the present study, we established a murine model of AR by sensitization with ovalbumin (OVA). Then, we investigated the mitochondrial morphology in mice with AR by transmission electron microscopy and confocal fluorescence microscopy, and evaluated the role of Mdivi‐1 (an inhibitor of mitochondrial fission) on allergic symptoms, inflammatory responses, allergic‐related signals, and reactive oxygen species formation.ResultsThere was a notable enhancement in mitochondrial fragmentation in the nasal mucosa of mice following OVA stimulation, whereas Mdivi‐1 prevented aberrant mitochondrial morphology. Indeed, Mdivi‐1 alleviated the rubbing and sneezing responses in OVA‐sensitized mice. Compared with vehicle‐treated ones, mice treated with Mdivi‐1 exhibited a reduction in interleukin (IL)‐4, IL‐5, and specific IgE levels in both serum and nasal lavage fluid, and shown an amelioration in inflammatory response of nasal mucosa. Meanwhile, Mdivi‐1 treatment was associated with a suppression in JAK2 and STAT6 activation and reactive oxygen species generation, which act as important signaling for allergic response.ConclusionOur findings reveal mitochondrial dynamics modulate the allergic responses in AR. Mitochondrial dynamics may represent a promising target for the treatment of AR.

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Ethanol extract of Dryopteris crassirhizoma alleviates allergic inflammation via inhibition of Th2 response and mast cell activation in a murine model of allergic rhinitis

Cited by 17 publications

References 47 publications

The extract of black cumin, licorice, anise, and black tea alleviates OVA-induced allergic rhinitis in mouse via balancing activity of helper T cells in lung

The extract of black cumin, licorice, anise, and black tea alleviates OVA-induced allergic rhinitis in mouse via balancing activity of helper T cells in lung

Topical Spilanthol Inhibits MAPK Signaling and Ameliorates Allergic Inflammation in DNCB-Induced Atopic Dermatitis in Mice

Mitochondrial dynamics modulate the allergic inflammation in a murine model of allergic rhinitis

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