Ethanol extract of Dendrobium chrysotoxum Lindl ameliorates diabetic retinopathy and its mechanism

Gong, Chenyuan; Yu, Zengyang; Lü, Bin; Yang, Li; Sheng, Yuchen; Fan, Yuanmin; Ji, Lili; Wang, Zhengtao

doi:10.1016/j.vph.2014.04.007

Cited by 45 publications

(29 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The schematic diagram of mechanism involved in the amelioration of DC against DR was shown in Figure 6 . The present study and previous study [ 14 ] demonstrate that D. chrysotoxum could ameliorate retinal inflammation, maintain retinal BRB, and attenuate retinal angiogenesis, thereby attenuating the development of DR. Thus, D. chrysotoxum may be recommended as a supplementary treatment for patients with DR.…”

Section: Discussionsupporting

confidence: 78%

“…One report demonstrated the amelioration of total alkaloids extracted from D. nobile on diabetic cataract in rats [ 16 ]. Recently, our previous study showed that D. chrysotoxum alleviated retinal angiogenesis during the development of DR [ 14 ]. Our results in this study showed that DC did not evidently affect body weight and blood glucose concentration of diabetic rats; however, 300 mg/kg DC alleviated retinal BRB breakdown in STZ-induced diabetic rats.…”

Section: Discussionmentioning

confidence: 99%

“…D. chrysotoxum was a gift from Jinling Pharmaceutical Co., LTD (Nanjing, China). The ethanol extract of D. chrysotoxum (DC) was prepared, as described in our previous study [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, the ethanol extract isolated from D. chrysotoxum (DC) alleviates retinal angiogenesis during the development of DR [ 14 ]. In the present study, we aimed to observe whether DC can ameliorate streptozotocin- (STZ-) induced DR in rats by alleviating retinal inflammation and restoring the decreased expression of tight junction proteins.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Dendrobium chrysotoxumLindl. Alleviates Diabetic Retinopathy by Preventing Retinal Inflammation and Tight Junction Protein Decrease

Gong

Lü

et al. 2015

Journal of Diabetes Research

Self Cite

View full text Add to dashboard Cite

Diabetic retinopathy (DR) is a serious complication of diabetes mellitus. This study aimed to observe the alleviation of the ethanol extract of Dendrobium chrysotoxum Lindl. (DC), a traditional Chinese herbal medicine, on DR and its engaged mechanism. After DC (30 or 300 mg/kg) was orally administrated, the breakdown of blood retinal barrier (BRB) in streptozotocin- (STZ-) induced diabetic rats was attenuated by DC. Decreased retinal mRNA expression of tight junction proteins (including occludin and claudin-1) in diabetic rats was also reversed by DC. Western blot analysis and retinal immunofluorescence staining results further confirmed that DC reversed the decreased expression of occludin and claudin-1 proteins in diabetic rats. DC reduced the increased retinal mRNA expressions of intercellular adhesion molecule-1 (ICAM-1), tumor necrosis factor α (TNFα), interleukin- (IL-) 6, and IL-1β in diabetic rats. In addition, DC alleviated the increased 1 and phosphorylated p65, IκB, and IκB kinase (IKK) in diabetic rats. DC also reduced the increased serum levels of TNFα, interferon-γ (IFN-γ), IL-6, IL-1β, IL-8, IL-12, IL-2, IL-3, and IL-10 in diabetic rats. Therefore, DC can alleviate DR by inhibiting retinal inflammation and preventing the decrease of tight junction proteins, such as occludin and claudin-1.

show abstract

Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

“…D. chrysotoxum was a gift from Jinling Pharmaceutical Co., LTD (Nanjing, China). The ethanol extract of D. chrysotoxum (DC) was prepared, as described in our previous study [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dendrobium chrysotoxumLindl. Alleviates Diabetic Retinopathy by Preventing Retinal Inflammation and Tight Junction Protein Decrease

Gong

Lü

et al. 2015

Journal of Diabetes Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…As the part of our continuing search for biologically active antiobesity agents from medicinal plants, a variety of plants from traditional Chinese medicine had been screened for their antilipase activity. Dendrobium officinale, which belongs to Orchidaceae, is well known for improving the symptoms of diabetes [5][6][7]. It was reported that the methanolic extract exhibited a pleiotropic effect on obesity-induced parameters and exerted a renoprotective effect in high-fat diet-fed mice [8].…”

Section: Introductionmentioning

confidence: 99%

Ultrafiltration coupled with high-performance liquid chromatography and quadrupole-time-of-flight mass spectrometry for screening lipase binders from different extracts of Dendrobium officinale

Tao

Cai

et al. 2015

Anal Bioanal Chem

View full text Add to dashboard Cite

Pancreatic lipase plays essential roles in the digestion, transport, and processing of dietary lipids in humans. Inhibition of pancreatic lipase leading to the decrease of lipid absorption may be used for treating obesity. In the present study, a new approach of ultrafiltration coupled with high-performance liquid chromatography and quadrupole-time-of-flight mass spectrometry was established for rapidly detecting lipase binders from different extracts of medicinal plants. Rutin, a model inhibitor of lipase, was selected to optimize the screening conditions, including ion strength, temperature, pH, and incubation time. Meanwhile, the specificity of the approach was investigated by using denatured lipase and inactive compound emodin. The optimal screening conditions were as follows: ion strength 75 mM, temperature 37 °C, pH 7.4, and incubation time 10 min. Furthermore, linearity, accuracy, precision, and matrix effect of the approach were well validated. Finally, lipase binders were screened from different extracts of Dendrobium officinale by applying the established approach and were subsequently subjected to traditional lipase inhibitory assay. Eleven lipase inhibitors were identified, eight of which, namely naringenine, vicenin II, schaftoside, isoschaftoside, isoquercetrin, kaempferol 3-O-β-D-glucopyranoside, vitexin 2″-O-glucoside, and vitexin 2″-O-rhamnoside, were reported for the first time. In addition, docking experiments were performed to determine the preferred binding sites of these new lipase inhibitors.

show abstract

Biotechnology Approaches on Characterization, Mass Propagation, and Breeding of Indonesian Orchids Dendrobium lineale (Rolfe.) and Vanda tricolor (Lindl.) with Its Phytochemistry

Semiarti

Purwantoro

Sari

2020

Reference Series in Phytochemistry

View full text Add to dashboard Cite

Ethanol extract of Dendrobium chrysotoxum Lindl ameliorates diabetic retinopathy and its mechanism

Cited by 45 publications

References 27 publications

Dendrobium chrysotoxumLindl. Alleviates Diabetic Retinopathy by Preventing Retinal Inflammation and Tight Junction Protein Decrease

Dendrobium chrysotoxumLindl. Alleviates Diabetic Retinopathy by Preventing Retinal Inflammation and Tight Junction Protein Decrease

Ultrafiltration coupled with high-performance liquid chromatography and quadrupole-time-of-flight mass spectrometry for screening lipase binders from different extracts of Dendrobium officinale

Biotechnology Approaches on Characterization, Mass Propagation, and Breeding of Indonesian Orchids Dendrobium lineale (Rolfe.) and Vanda tricolor (Lindl.) with Its Phytochemistry

Contact Info

Product

Resources

About