Ethanol exposure during gastrulation alters neuronal morphology and behavior in zebrafish

Shan, Shubham; Boutin, Savanna; Ferdous, Jannatul; Ali, Declan W.

doi:10.1016/j.ntt.2015.01.004

Cited by 16 publications

(12 citation statements)

References 49 publications

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“…Exposure to 1% ethanol reduces the number of elavl3-positive neural progenitors 54 and 100 mM (0.6%) increases the branching of secondary motor neurons and decreased the diameter of Mauthner axons. 55 In addition, Zhang et al 56 reported that 0.5% ethanol exposure leads to disruption of the mid-hindbrain boundary. These and other structural brain abnormalities have been found to have an adverse relationship with cognitive function and behavior in the clinical population.…”

Section: Zebrafish Models Of Fasdmentioning

confidence: 99%

“…Zebrafish larvae demonstrate a rapid escape response/startle response known as a C-start. 69 Shan et al 55 exposed embryos from 5.25 to 10.75 hpf to ethanol concentration of 0%, 0.006%, 0.3%, and 0.6%, and measured C-starts among other criteria in 2-day-old larvae. They found that ethanol-treated fish showed an increase in abnormal C-starts and tail speed.…”

Section: Zebrafish Models Of Fasdmentioning

confidence: 99%

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Fishing for Fetal Alcohol Spectrum Disorders: Zebrafish as a Model for Ethanol Teratogenesis

2016

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Fetal Alcohol Spectrum Disorders (FASD) describes a wide array of ethanol-induced developmental defects, including craniofacial dysmorphology and cognitive impairments. It affects *1 in 100 children born in the United States each year. Due to the pleiotropic effects of ethanol, animal models have proven critical in characterizing the mechanisms of ethanol teratogenesis. In this review, we focus on the utility of zebrafish in characterizing ethanolinduced developmental defects. A growing number of laboratories have focused on using zebrafish to examine ethanol-induced defects in craniofacial, cardiac, ocular, and neural development, as well as cognitive and behavioral impairments. Growing evidence supports that genetic predisposition plays a role in these ethanol-induced defects, yet little is understood about these gene-ethanol interactions. With a high degree of genetic amenability, zebrafish is at the forefront of identifying and characterizing the gene-ethanol interactions that underlie FASD.

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Section: Zebrafish Models Of Fasdmentioning

confidence: 99%

Section: Zebrafish Models Of Fasdmentioning

confidence: 99%

Fishing for Fetal Alcohol Spectrum Disorders: Zebrafish as a Model for Ethanol Teratogenesis

2016

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“…Escape responses of 2 dpf embryos were tested and recorded as previously described [24]. Briefly, 2 dpf embryos were immobilized in 2% low-melting point agarose (LMPA; Sigma-Aldrich; St. Louis, MO, USA) dissolved in embryo medium.…”

Section: Escape Response In 2 Dpf Embryosmentioning

confidence: 99%

Early Exposure to THC Alters M-Cell Development in Zebrafish Embryos

2020

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Cannabis is one of the most commonly used illicit recreational drugs that is often taken for medicinal purposes. The psychoactive ingredient in cannabis is Δ9-Tetrahydrocannabinol (Δ9-THC, hereafter referred to as THC), which is an agonist at the endocannabinoid receptors CB1R and CB2R. Here, we exposed zebrafish embryos to THC during the gastrulation phase to determine the long-term effects during development. We specifically focused on reticulospinal neurons known as the Mauthner cells (M-cell) that are involved in escape response movements. The M- cells are born during gastrulation, thus allowing us to examine neuronal morphology of neurons born during the time of exposure. After the exposure, embryos were allowed to develop normally and were examined at two days post-fertilization for M-cell morphology and escape responses. THC treated embryos exhibited subtle alterations in M-cell axon diameter and small changes in escape response dynamics to touch. Because escape responses were altered, we also examined muscle fiber development. The fluorescent labelling of red and white muscle fibers showed that while muscles were largely intact, the fibers were slightly disorganized with subtle but significant changes in the pattern of expression of nicotinic acetylcholine receptors. However, there were no overt changes in the expression of nicotinic receptor subunit mRNA ascertained by qPCR. Embryos were allowed to further develop until 5 dpf, when they were examined for overall levels of movement. Animals exposed to THC during gastrulation exhibited reduced activity compared with vehicle controls. Together, these findings indicate that zebrafish exposed to THC during the gastrula phase exhibit small changes in neuronal and muscle morphology that may impact behavior and locomotion.

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“…Furthermore, the c-start is a rapid escape / startle response that very young (2 day old) zebrafish display (Roberts et al 2011). Exposure to 0.6% ethanol from 5.25 hpf to 10.75 hpf caused an increase in abnormal c-starts and tail speed, suggesting hyperactivity (Shan et al 2015). Mauthner neurons mediate the c-start and following treatment with 0.6% these neurons have smaller cell bodies and axons (Shan et al 2015).…”

Section: Behavior and Cognitionmentioning

confidence: 99%

“…Exposure to 0.6% ethanol from 5.25 hpf to 10.75 hpf caused an increase in abnormal c-starts and tail speed, suggesting hyperactivity (Shan et al 2015). Mauthner neurons mediate the c-start and following treatment with 0.6% these neurons have smaller cell bodies and axons (Shan et al 2015). Future study is needed to explain the opposing outcomes of these studies.…”

Section: Behavior and Cognitionmentioning

confidence: 99%

Diving into the world of alcohol teratogenesis: a review of zebrafish models of fetal alcohol spectrum disorder

Fernandes

Buckley

Eberhart

2018

Biochem. Cell Biol.

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The term fetal alcohol spectrum disorder (FASD) refers to the entire suite of deleterious outcomes resulting from embryonic exposure to alcohol. Along with other reviews in this special issue, we provide insight into how animal models, specifically the zebrafish, have informed our understanding of FASD. We first provide a brief introduction to FASD. We discuss the zebrafish as a model organism and its strengths for alcohol research. We detail how zebrafish has been used to model some of the major defects present in FASD. These include behavioral defects, such as social behavior as well as learning and memory, and structural defects, disrupting organs such as the brain, sensory organs, heart, and craniofacial skeleton. We provide insights into how zebrafish research has aided in our understanding of the mechanisms of ethanol teratogenesis. We end by providing some relatively recent advances that zebrafish has provided in characterizing gene-ethanol interactions that may underlie FASD.

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Ethanol exposure during gastrulation alters neuronal morphology and behavior in zebrafish

Cited by 16 publications

References 49 publications

Fishing for Fetal Alcohol Spectrum Disorders: Zebrafish as a Model for Ethanol Teratogenesis

Fishing for Fetal Alcohol Spectrum Disorders: Zebrafish as a Model for Ethanol Teratogenesis

Early Exposure to THC Alters M-Cell Development in Zebrafish Embryos

Diving into the world of alcohol teratogenesis: a review of zebrafish models of fetal alcohol spectrum disorder

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