Ethanol enhances retinoic acid metabolism into polar metabolites in rat liver via induction of cytochrome P4502E1

Li, Chun; Russell, Robert M.; Seitz, Helmut K.; Wang, Xiang Dong

doi:10.1053/gast.2001.20877

Cited by 119 publications

(105 citation statements)

References 43 publications

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“…Ethanol decreases RA synthesis in competition with retinol to inhibit the alcohol-dehydrogenase (Duester, 1991;Duester et al, 1991). In addition, RA can be degraded by ethanol inducible CYP2E1, a P450 metabolizing RA, to more polar metabolites in vitro (Liu et al, 2001). This process would need days or weeks of treatment to occur, which does not correspond to our experimental conditions.…”

Section: Discussionmentioning

confidence: 99%

Retinoid‐dependent mRNA expression and poly‐(A) contents in bovine oocytes meiotically arrested and/or matured in vitro

Gómez

Rodríguez

Dd́ez

et al. 2004

Molecular Reproduction Devel

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The presence of retinoic acid (RA) during in vitro maturation (IVM) improves bovine oocyte quality and developmental potential. In this work, we investigated the underlying molecular mechanisms. Cumulus-oocyte complexes were meiotically arrested by roscovitine and/or matured in defined medium containing RA, 1% ethanol (vehicle), or no additives. Cumulus-free oocytes were analyzed for poly-(A) mRNA contents and relative mRNA expression of genes involved in cell cycle regulation (cyclin B1 and H1) and antioxidative defence (Mn-superoxide dismutase and glucose-6-phosphate dehydrogenase). Poly-(A) mRNA increased after meiotic inhibition and decreased with IVM completion, both in meiotically arrested and permissively matured oocytes, i.e., matured without previous meiotic arrest. RA dramatically increased poly-(A) mRNA in meiotically arrested oocytes, but more than half of the poly-(A) mRNA disappeared during maturation. Irrespective of oocyte origin, transcripts were detected for all the genes analyzed. IVM, with or without previous meiotic inhibition, increased expression of cyclin B1 and glucose-6-phosphate dehydrogenase, and decreased cyclin H1 and Mn-superoxide dismutase. Except for a decreasing of Mn-superoxide dismutase in meiotically arrested and matured oocytes, RA did not affect mRNA expression. Ethanol led to an abnormal poly-(A) mRNA profile and expression of all the genes analyzed. RA does not modify expression of cyclin B1 and HI genes in the bovine oocyte, and probably does not generate oxidative stress. In addition, RA enhanced mRNA amount as measured by poly-(A) mRNA contents.

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Section: Discussionmentioning

confidence: 99%

Retinoid‐dependent mRNA expression and poly‐(A) contents in bovine oocytes meiotically arrested and/or matured in vitro

Gómez

Rodríguez

Dd́ez

et al. 2004

Molecular Reproduction Devel

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“…In addition to the three CYP26 enzymes, several members of other CYP families, including human CYP3A4, CYP3A5, CYP3A7, CYP1A1, CYP4A11, CYP2C8, CYP2C9, CYP2C22, CYP2C39, CYP2S1, and CYP2E1 (136)(137)(138)(139)(140)(141)(142)(143)(144)(145)(146)(147)(148)(149)(150), have been shown to catabolize atRA. While CYP2C8 is catalytically more effi cient than either CYP2C9 or CYP3A4, the overall contribution of CYP2C8 to atRA hydroxylation may not be much greater than that of CYP3A4 and CYP2C9, because the two latter enzymes are expressed at about 10-fold higher levels than CYP2C8 ( 139 ).…”

Section: Biosynthesis Of Atra From ␤ -Carotenementioning

confidence: 99%

“…Because chronic alcohol administration can lead to ‫ف‬ 10-fold elevation of CYP2E1 activity, this enzyme may have a signifi cant impact on atRA catabolism in alcoholic liver disease. In fact, ethanol was shown to enhance atRA metabolism into polar metabolites through the induction of CYP2E1 in the liver ( 149,150 ).…”

Section: Biosynthesis Of Atra From ␤ -Carotenementioning

confidence: 99%

Enzymology of retinoic acid biosynthesis and degradation

Kedishvili

2013

Journal of Lipid Research

166

165

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This article is available online at http://www.jlr.org ( 10 ), and regulation of energy homeostasis ( 11,12 ). atRA exerts its actions by serving as an activating ligand of nuclear atRA receptors [retinoid acid receptor (RAR) ␣ , RAR ␤ , and RAR ␥ ] and peroxisome proliferator-activated receptors (PPAR) ␤ / ␦ , which form heterodimers with retinoid X receptors ( 13,14 ). The actions of the RARs are described in more detail in this thematic series by RochetteEgly and colleagues. The concentration of atRA during embryonic development is tightly controlled in a spatial and temporal manner, and in adult tissues, it is maintained within a very narrow range that is specifi c for each given tissue. If the control mechanisms fail and the concentration of atRA exceeds or falls below the optimal range, tissues and cells undergo pathophysiological changes that in most severe cases can lead to disease. Thus, the maintenance of optimal atRA levels is essential for life. This review focusses on recent fi ndings regarding the mechanisms that control atRA homeostasis, with specifi c emphasis on the enzymes involved in atRA biosynthesis and degradation. All-trans -retinoic acid (atRA) is a highly potent derivative of vitamin A that is required for virtually all essential physiological processes and functions because of its involvement in transcriptional regulation of over 530 different genes ( 1, 2 ). For example, atRA is necessary for differentiation and development of fetal and adult tissues, stem cell differentiation, and apoptosis ( 3-7 ), and for support of reproductive functions ( 8, 9 ), immune response

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“…[39][40][41] Acetaldehyde, a toxic molecule, is subsequently converted to nontoxic acetates by mitochondrial acetaldehyde dehydrogenase. 39,41 Additionally, CYP2E1 enables the clearance of ethanol through an oxidative reaction [41][42][43] (Fig. 4).…”

Section: à5mentioning

confidence: 99%

A Comparative Study of Genome-Wide Transcriptional Profiles of Primary Hepatocytes in Collagen Sandwich and Monolayer Cultures

Kim

Lasher

Milford

et al. 2010

Tissue Engineering Part C: Methods

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Two commonly used culture systems in hepatic tissue engineering are the collagen sandwich (CS) and monolayers of cells. In this study, genome-wide gene expression profiles of primary hepatocytes were measured over an 8-day period for each cell culture system using Affymetrix GeneChips and compared via gene set enrichment analysis to elicit biologically meaningful information at the level of gene sets. Our results demonstrate that gene expression in hepatocytes in CS cultures steadily and comprehensively diverges from that in monolayer cultures. Gene sets up-regulated in CS cultures include several associated with liver metabolic and synthesis functions, such as metabolism of lipids, amino acids, carbohydrates, and alcohol, and synthesis of bile acids. Monooxygenases such as Cytochrome-P450 enzymes do not show any change between the culture systems after 1 day, but exhibit significant up-regulation in CS cultures after 3 days in comparison to hepatocyte monolayers. These data provide insights into the up-and down-regulation of several liver-critical gene sets and their subsequent effects on liver-specific functions. These results provide a baseline for further explorations into the systems biology of engineered liver mimics.

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Ethanol enhances retinoic acid metabolism into polar metabolites in rat liver via induction of cytochrome P4502E1

Cited by 119 publications

References 43 publications

Retinoid‐dependent mRNA expression and poly‐(A) contents in bovine oocytes meiotically arrested and/or matured in vitro

Retinoid‐dependent mRNA expression and poly‐(A) contents in bovine oocytes meiotically arrested and/or matured in vitro

Enzymology of retinoic acid biosynthesis and degradation

A Comparative Study of Genome-Wide Transcriptional Profiles of Primary Hepatocytes in Collagen Sandwich and Monolayer Cultures

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