Ethanol binge drinking during pregnancy and its effects on salivary glands of offspring rats: oxidative stress, morphometric changes and salivary function impairments

Ferreira, Railson de Oliveira; Aragão, Walessa Alana Bragança; Bittencourt, Leonardo Oliveira; Fernandes, Luanna Melo Pereira; Balbinot, Karolyny Martins; Alves-Júnior, Sérgio de Melo; Pinheiro, João de Jesus Viana; Maia, Cristiane do Socorro Ferraz; Crespo-López, María Elena; Lima, Rafael Rodrigues

doi:10.1016/j.biopha.2020.110979

Cited by 9 publications

(18 citation statements)

References 48 publications

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“…As demonstrated by, admittedly, few studies, this method of drinking results in salivary gland dysregulation and oral redox imbalance. Ferreira et al (2021) investigated the effect of binge drinking during critical periods of pregnancy on the salivary glands of offspring in rats. Ethanol was administered during gestational days: GD6, GD7, GD8, i.e., during gastrulation and neurulation.…”

Section: Alcohol and Reactive Oxygen Speciesmentioning

confidence: 99%

Ethanol- and Cigarette Smoke-Related Alternations in Oral Redox Homeostasis

2022

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Alcohol abuse as well as smoking cigarettes has been proven to negatively affect the oral environment. The aim of this work was to provide a systematic review of the literature on the influence of ethanol and cigarette smoking on oral redox homeostasis. A search was performed for scientific articles indexed in the PubMed, Medline and Web of Science databases. We identified 32,300 articles, of which 54 were used for the final review, including the results from 2000 to 2021. Among the publications used to write this article, n = 14 were related to the influence of alcohol consumption (clinical studies n = 6, experimental studies n = 8) and n = 40 were related to the influence of smoking (clinical studies n = 33, experimental studies n = 7) on oral redox homeostasis. The reviewed literature indicates that alcohol abusers and smokers are more likely to suffer from salivary gland dysfunction, as well as develop precancerous lesions due to DNA damage. Compared to alcohol abstainers and non-smokers, alcohol drinkers and smokers are also characterized by a deterioration in periodontal health measured by various indicators of periodontal status. In summary, alcohol abuse and smoking are associated with disrupted oral redox homeostasis, which may lead not only to tooth loss, but also contribute to various adverse effects related to mental health, digestive processes and chronic inflammation throughout the human body.

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Section: Alcohol and Reactive Oxygen Speciesmentioning

confidence: 99%

Ethanol- and Cigarette Smoke-Related Alternations in Oral Redox Homeostasis

2022

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“…EtOH induces detrimental effects through different mechanisms, such as excitotoxicity, neuroinflammation, and oxidative stress [21]. A study on prenatal alcohol intake has shown that the number and the morphology of MN were significantly reduced.…”

Section: Discussionmentioning

confidence: 99%

“…Heavy episodic drinking (binge), in which the EtOH concentration reaches at least 0.08 g per deciliter of blood, has grown significantly among adolescents and young adults [12][13][14][15][16][17][18][19][20]. In animal studies, binge-like EtOH consumption has been associated with tissue changes and oxidative stress in salivary glands [21,22], hippocampus, and prefrontal cortex, damage of motor and cognitive functions [23][24][25], and decrease of alveolar bone quality [26,27]. One of the main harmful effects of EtOH is the increase of reactive oxygen species (ROS) and decrease of antioxidants such as the glutathione peroxidase (GPx) enzyme [28,29].…”

Section: Introductionmentioning

confidence: 99%

Aerobic Physical Training Attenuates Oxidative Stress in the Spinal Cord of Adult Rats Induced by Binge-like Ethanol Intake

et al. 2023

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Binge drinking is the most frequent consumption pattern among young adults and remarkably changes the central nervous system; thus, research on strategies to protect it is relevant. This study aimed to investigate the detrimental effects of binge-like EtOH intake on the spinal cord of male rats and the potential neuroprotective effects provided by moderate-intensity aerobic physical training. Male Wistar rats were distributed into the ‘control group’, ‘training group’, ‘EtOH group’, and ‘training + EtOH’. The physical training protocol consisted of daily 30-min exercise on a treadmill for 5 consecutive days followed by 2 days off during 4 weeks. After the fifth day of each week, distilled water (‘control group’ and ‘training group’) or 3 g/kg of EtOH diluted at 20% w/v (‘EtOH group’ and ‘training + EtOH group’) was administered for 3 consecutive days through intragastric gavage to simulate compulsive consumption. Spinal cord samples were collected for oxidative biochemistry and morphometric analyses. The binge-like EtOH intake induced oxidative and tissue damage by decreasing reduced glutathione (GSH) levels, increasing lipid peroxidation (LPO), and reducing motor neurons (MN) density in the cervical segment. Even under EtOH exposure, physical training maintained GSH levels, reduced LPO, and prevented MN reduction at the cervical segment. Physical training is a non-pharmacological strategy to neuroprotect the spinal cord against oxidative damage induced by binge-like EtOH intake.

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“…For this analysis, images were taken by a color digital camera (Cyber Shot DSC W-230, 4× optical zoom, Sony, Tokyo, Japan) coupled to a microscope (1.5×, Eclipse E200, Nikon, Tokyo, Japan; in a 40× magnification) of 5 random transverse sections of the glands, with 3 fields of each section being evaluated. The direct variables of tissue morphometric evaluation, expressed in µm 2 , were: the acinar area, ductal areal, parenchyma area, and stromal area [40,59,60]. Variable values were obtained using a digital image analyzer, Image J software (NIMH, NIH, Bethesda, MD, USA)…”

Section: Histological Evaluation 451 Morphometric Analysesmentioning

confidence: 99%

Maternal Fluoride Exposure Exerts Different Toxicity Patterns in Parotid and Submandibular Glands of Offspring Rats

Santos

Ferreira

Bittencourt

et al. 2022

IJMS

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There is currently a controversial and heated debate about the safety and ethical aspects of fluoride (F) used for human consumption. Thus, this study assessed the effects of prenatal and postnatal F exposure of rats on the salivary glands of their offspring. Pregnant rats were exposed to 0, 10, or 50 mg F/L from the drinking water, from the first day of gestation until offspring weaning (42 days). The offspring rats were euthanized for the collection of the parotid (PA) and submandibular (SM) glands, to assess the oxidative biochemistry and to perform morphometric and immunohistochemical analyses. F exposure was associated with a decrease in the antioxidant competence of PA in the 10 mg F/L group, contrasting with the increase observed in the 50 mg F/L group. On the other hand, the antioxidant competence of the SM glands was decreased at both concentrations. Moreover, both 10 and 50 mg F/L groups showed lower anti-α-smooth muscle actin immunostaining area in SM, while exposure to 50 mg F/L was associated with changes in gland morphometry by increasing the duct area in both glands. These findings demonstrate a greater susceptibility of the SM glands of the offspring to F at high concentration in comparison to PA, reinforcing the need to adhere to the optimum F levels recommended by the regulatory agencies. Such findings must be interpreted with caution, especially considering their translational meaning.

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Ethanol binge drinking during pregnancy and its effects on salivary glands of offspring rats: oxidative stress, morphometric changes and salivary function impairments

Cited by 9 publications

References 48 publications

Ethanol- and Cigarette Smoke-Related Alternations in Oral Redox Homeostasis

Ethanol- and Cigarette Smoke-Related Alternations in Oral Redox Homeostasis

Aerobic Physical Training Attenuates Oxidative Stress in the Spinal Cord of Adult Rats Induced by Binge-like Ethanol Intake

Maternal Fluoride Exposure Exerts Different Toxicity Patterns in Parotid and Submandibular Glands of Offspring Rats

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