Ethanol, 30 Per Cent, and Hamster Pouch Carcinogenesis

Abstract

SYNOPSIS IN INTERLINGUA ETHANOL DE TRENTA PRO CENTO E CARCINOGENESE IN LE BURSA BUCCAL DEL HAMSTER.-Le influentia deethanol in promover le disveloppamento de carcinoma oral, un effect que se suggere clinicamente, esseva studiate experimentalmente con le utilisation de 60 hamsters auree. Le gruppo experimental de animals recipeva ethanol in un concentration de 30 pro cento como le sol provision de liquido. Le gruppo del animals de controlo recipeva aqua. Le carcinogens, 9, 10-dimethyl-1,2 benzanthraceno in oleo mineral esseva applicate in mesurate quantitates al superficie interior del bursas buccal. Post 72 dies, le incidentia de tumores maligne esseva plus alte in le gruppo recipients ethanol. Degeneration grassiose del hepate esseva prevalente in iste gruppo. Studios additional es necessari pro confirmar le presente resultatos. Recent studies'-3 have indicated that a significant number of individuals with oral carcinoma have a history of prolonged and immoderate consumption of alcoholic beverages. These findings suggested a correlation between chronic alcoholism and oral carcinoma. A thorough review of the literature failed to reveal a previous experimental approach to this problem. Therefore, an investigation was designed to study the development of chemically induced oral carcinoma in the experimental animal as influenced by the ingestion of dilute ethyl alcohol. If it were found that alcohol promoted the formation of induced carcinoma of the oral mucous membranes, subsequent studies might be directed toward elucidating the mechanisms involved.In 1956, a study2 was designed to investigate the validity of observations which indicated that chronic alcoholism plays a role in the etiology of oral carcinoma. A review of case histories of 115 patients with oral epidermoid carcinoma revealed that 60 per cent of the patients used excessive quantities of whiskey, ranging from 1 quart a week to 12 ounces a day. A review of histories from a cancer-free control group demonstrated that only 29 per cent consumed equivalent amounts. The author suggested that alcohol may contribute as an extrinsic local irritant or as an intrinsic factor through its influence on normal liver function.A more striking correlation was estab-

Ethanol Promotes Chemically Induced Oral Cancer in Mice through Activation of the 5-Lipoxygenase Pathway of Arachidonic Acid Metabolism

“…Several previous animal studies failed to statistically confirm the cancer-promoting activity of ethanol on DMBA-induced hamster cheek pouch carcinogenesis (13–16). In our study, mice were given 8% ethanol ad libitum as the sole drink based on the following rationale: a) drinking is a method of administration best resembling human behavior (22).…”

“…At this age, Sprague-Dawley rats spontaneously develop oral cancer even without any other treatment (12). Several studies in the literature suggested, but failed to confirm statistically, the cancer-promoting activity of ethanol feeding or painting in 7,12-dimethylbenz[ a ]anthracene (DMBA)-induced hamster cheek pouch carcinogenesis (13–16). Therefore, it is necessary to develop a proper animal model to investigate whether ethanol may promote oral carcinogenesis, and to use such a model to elucidate the molecular mechanism of ethanol-related oral cancer.…”

Chronic ethanol ingestion and the hepatocarcinogenicity of N‐hydroxy‐N‐2‐fluorenylacetamide

“…Nonetheless, the level of lipid in the diet, which was low in our experiments and in those of Schmahl, may be more relevant and the effect of this factor, together with alcoholic beverages and carcinogens may be worth further examination. Of course, more sensitive species deserve consideration in such additional tests (Schmahl et al, 1965;Henefer, 1966;Weisburger and Weisburger, 1967).…”

Initiation and promotion of experimental oral mucosal carcinogenesis in mice

“…In the field of experimental oral mueosal eareinogenesis, several studies of eo-carcinogenesis, rather than strietly two-stage eareinogenesis, have been undertaken (. [5][6][7][8], some with eontradietory results. When hamsters treated with dimethylbenz-anthracene (DMBA) are given a 30"/o v/v aqueous solution of cthanol instead of water to drink no difference in latent period or numbers of neoplasms in the eheek pouches can be demonstrated (7).…”