Ethane 1,2-dimethane Sulfonate Effects on the Testis of the Lizard, Podarcis s. sicula Raf: Morphological and Hormonal Changes

Minucci, Sergio; Fasano, Silvia; Marmorino, C; Chieffi, Paolo; Pierantoni, Riccardo

doi:10.1006/gcen.1995.1027

Cited by 11 publications

(5 citation statements)

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“…In neonatal rats dosed (Postnatal Day [PND] [5][6][7][8][9][10][11][12][13][14][15][16] s.c. with 50 mg/kg EDS, the fetal LC population is destroyed and the seminiferous tubules were permanently damaged either by direct cytotoxic effects prior to the formation of the blood-testis barrier or androgen deprivation resulting from fetal LC loss [13]. Furthermore, EDS was cytotoxic to adult LCs in the testis of the guinea pig [4], frog [14], rabbit [15], and lizard [16]. LCs in the adult Syrian hamster [17] and immature rat [18] exhibit significant effects of EDS exposure, although they are considered less sensitive than adult rat LCs based on the relative decrease in T production by LCs incubated in vitro with EDS.…”

Section: Introductionmentioning

confidence: 99%

Gestational Exposure to Ethane Dimethanesulfonate Permanently Alters Reproductive Competence in the CD-1 Mouse1

Tarka-Leeds¹,

Suarez²,

Roberts³

et al. 2003

Biology of Reproduction

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Although the adult mouse Leydig cell (LC) has been considered refractory to cytotoxic destruction by ethane dimethanesulfonate (EDS), the potential consequences of exposure during reproductive development in this species are unknown. Herein pregnant CD-1 mice were treated with 160 mg/kg on Gestation Days 11-17, and reproductive development in male offspring was evaluated. Prenatal administration of EDS compromised fetal testosterone (T) levels, compared with controls. EDS-exposed pups recovered their steroidogenic capacities after birth because T production by hCG-stimulated testis parenchyma from prepubertal male offspring was unchanged. However, prepubertal testes from prenatally exposed males contained seminiferous tubules (STs) devoid of germ cells, indicating a delay in spermatogenesis. In adults, some STs in exposed males still contained incomplete germ cell associations corroborating observed reductions in epididymal sperm reserves, fertility ratios, and litter size. Morphometry revealed an EDS-induced increase in interstitial area and a concomitant decrease in ST area, but stereology revealed an unexpected decrease in the number and size of the LCs per testis in exposed males. Paradoxically, there was an increase in both serum LH and T production by adult testis parenchyma, indicating that the LCs were hyperstimulated. These data demonstrate permanent lesions in LC development and spermatogenesis caused by prenatal exposure in mice. Thus, although adult mouse LCs are insensitive to EDS, EDS appears to have direct action on fetal LCs, resulting in abnormal testis development.

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Section: Introductionmentioning

confidence: 99%

Gestational Exposure to Ethane Dimethanesulfonate Permanently Alters Reproductive Competence in the CD-1 Mouse1

Tarka-Leeds¹,

Suarez²,

Roberts³

et al. 2003

Biology of Reproduction

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“…This coincides with the maximal interstitial tissue damage and indicates that Leydig cell activity was greatly impaired (Minucci etal, 1995). On day 28 after EDS injection, a new population of mature Leydig cells is seen in the interstitium together with a slight but significant increase in plasma androgen level (Minucci et al, 1995). Contemporaneous with these observations, MCN returns to control values.…”

Section: Discussionmentioning

confidence: 54%

“…MCN increased significantly on day 3 after EDS treatment (p < 0.01) ( Fig. 3), concomitant with the destruction of Leydig cells in large areas of interstitial tissue, while in the spermatogenic tubules close to them some pyknotic nuclei of spermatocytes appeared, and empty spaces were seen at the edge of the tubule (Minucci et al, 1995).…”

Section: Experiments 2: Eds Treatmentmentioning

confidence: 90%

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Mast cell-Leydig cell relationships in the testis of the lizard podarcis s. sicula Raf: thermal manipulation, ethane 1,2-dimethane sulphonate (EDS) and sex hormone treatment

Minucci

Vitiello

Marmorino

et al. 1995

Zygote

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SummaryMast cells of connective tissue type are scattered in the interstitial compartment of the lizard Podarcis s. sicula. Their number varies during the year, showing peaks in spring and in winter, respectively.Thermal manipulation affects mast cell number (MCN): high temperature decreased MCN in both January and May, while low temperature increased MCN only in January. Ethane dimethane sulphonate, a toxin which specifically destroys Leydig cells, induced an increase in MCN on days 3 and 7 of treatment. Oestradiol treatment provoked a strong increase in MCN that was blocked by tamoxifen. Blocking androgen receptors with cyproterone acetate resulted in an increase in MCN, while testosterone injection provoked a strong decrease. These results suggest a relationship between the presence of mature Leydig cells and mast cell proliferation and/or differentiation.

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“…This conclusion was further confirmed by other researchers (Zang et al, 2017;Zhang M.et al, 2017). In addition, EDS had effects on ALCs in the testis of the guinea pig (Kerr et al, 1987), frog (Minucci et al, 1990), rabbit (Laskey et al, 1994), and lizard (Minucci et al, 1995). In this study, EDS could eliminate differentiated LCs in pigs, which was accompanied with a decreased expression of CYP17A1, whereas the expression of PDGFRα did not change.…”

Section: Discussionmentioning

confidence: 56%

miR-205 Expression Elevated With EDS Treatment and Induced Leydig Cell Apoptosis by Targeting RAP2B via the PI3K/AKT Signaling Pathway

Yang

Chen

et al. 2020

Front. Cell Dev. Biol.

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The adult Leydig cells (ALCs), originated from stem Leydig cells (SLCs), can secrete testosterone which is essential for germ cell development and sexual behavior maintenance. As a synthetic compound, ethane dimethane sulfonate (EDS), a wellknown alkylating agent, has been reported to specifically ablate ALCs. In this study, EDS was verified to ablate differentiated pig LCs by experiments. Subsequently, the primary isolated pig LCs (containing SLCs and differentiated LCs) and EDS-treated LCs (almost exclusively SLCs) were collected for RNA-seq 4,904 genes and 15 miRNAs were differently expressed between the two groups. Down-regulated genes in the EDS-treated group were mainly related to steroid hormone biosynthesis. The highest up-regulation miRNAs was miR-205 after EDS treatment. Additionally, miR-205 was expressed more highly in pig SLCs clones compared with differentiated LCs. Through qRT-PCR, western blot (WB), TUNEL, EDU and flow cytometry, miR-205 was found to induce cell apoptosis, but did not affect proliferation or differentiation in both TM3 and GC-1spg mouse cell lines. Through luciferase reporter assays and WB, RAP2B was identified as a target gene of miR-205. Besides, overexpression of miR-205 inhibited the expressions of PI3K, Akt and p-AKT. All these findings were helpful for elucidating the regulation mechanism in pig LCs.

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Ethane 1,2-dimethane Sulfonate Effects on the Testis of the Lizard, Podarcis s. sicula Raf: Morphological and Hormonal Changes

Cited by 11 publications

References 0 publications

Gestational Exposure to Ethane Dimethanesulfonate Permanently Alters Reproductive Competence in the CD-1 Mouse1

Gestational Exposure to Ethane Dimethanesulfonate Permanently Alters Reproductive Competence in the CD-1 Mouse1

Mast cell-Leydig cell relationships in the testis of the lizard podarcis s. sicula Raf: thermal manipulation, ethane 1,2-dimethane sulphonate (EDS) and sex hormone treatment

miR-205 Expression Elevated With EDS Treatment and Induced Leydig Cell Apoptosis by Targeting RAP2B via the PI3K/AKT Signaling Pathway

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