Etd1p is a novel protein that links the SIN cascade with cytokinesis

Daga, Rafael R.; Lahoz, Agustín; Muñoz, Manuel J.; Moreno, Sergio; Jiménez, Juan

doi:10.1038/sj.emboj.7600705

Cited by 29 publications

(73 citation statements)

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“…At restrictive growth temperatures, these etd1-deficient mutants exhibit a conventional sin-deficient phenotype, yielding elongated and multinucleate cells without a septum ( Jimenez and Oballe 1994;Daga et al 2005;Garcia-Cortes and McCollum 2009).…”

Section: Resultsmentioning

confidence: 99%

“…In agreement with this analysis, we found that a pab1D null allele also suppressed lethality of etd1-deficient cells (data not shown). Since Etd1 is a positive regulator of SIN (Daga et al 2005;Garcia-Cortes and McCollum 2009), the ability of pab1-4 and pab1D to suppress etd1 mutants suggests that Pab1 might act as an inhibitor of the SIN pathway.…”

Section: Resultsmentioning

confidence: 99%

“…Image analysis: Fluorescence intensity measurements for Cdc7-GFP were made using ImageJ software (National Institutes of Health, Bethesda, MD) by placing a circle around SPBlocalized Cdc7 and measuring the maximal fluorescence (Daga et al 2005). A nearby cytosolic region was used as background.…”

Section: Methodsmentioning

confidence: 99%

“…For regulated nmt expression, described methods were used (Maundrell 1993). Conditional deficiency for etd1 was assessed by using the etd1-1 allele (37°or 6% v/v ethanol are permissive conditions), the null etd1D allele (37°, permissive temperature), or the etd1D nmt81x-etd1 strain at any growth temperature, where etd1 expression is regulated by thiamine under the weak repressible nmt81x promoter (Daga et al 2005). This later strain was used to produce etd1D mutants with the desired genetic background, removing the nmt81x-etd1 marker by tetrad dissection.…”

Section: Methodsmentioning

confidence: 99%

“…Further characterization indicated that Etd1 acts as a positive regulator of the SIN (Daga et al 2005). A recent study has established a key role for Etd1 in the timing of cytokinesis via the regulation of Spg1, acting as a potential homolog of budding yeast Lte1 (Garcia-Cortes and McCollum 2009).…”

Section: T He Fission Yeast Schizosaccharomyces Pombe Is a Lead-mentioning

confidence: 99%

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Antagonistic Roles of PP2A-Pab1 and Etd1 in the Control of Cytokinesis in Fission Yeast

et al. 2010

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In Schizosaccharomyces pombe, Etd1 is a positive regulator of the septation initiation network (SIN), a conserved GTPase-regulated kinase cascade that triggers cytokinesis. Here we show that a mutation in the pab1 gene, which encodes the B-regulatory subunit of the protein phosphatase 2A (PP2A), suppresses mutations in the etd1 gene. Etd1 is required for the function of the GTPase Spg1, a key regulator of SIN signaling. Interestingly, the loss of Pab1 function restored the activity of Spg1 in Etd1-deficient cells. This result suggests that PP2A-Pab1-mediated dephosphorylation inhibits Spg1, thus antagonizing Etd1 function. The loss of pab1 function also rescues the lethality of mutants of other genes in the SIN cascade such as mob1, sid1, and cdc11. Two-hybrid assays indicate that Pab1 physically interacts with Mob1, Sid1, Sid2, and Cdc11, suggesting that the phosphatase 2A B-subunit is a component of the SIN complex. Together, our results indicate that PP2A-Pab1 plays a novel role in cytokinesis, regulating SIN activity at different levels. Pab1 is also required to activate polarized cell growth. Thus, PP2A-Pab1 may be involved in coordinating polar growth and cytokinesis.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: T He Fission Yeast Schizosaccharomyces Pombe Is a Lead-mentioning

confidence: 99%

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Antagonistic Roles of PP2A-Pab1 and Etd1 in the Control of Cytokinesis in Fission Yeast

et al. 2010

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Dividing the spoils of growth and the cell cycle: The fission yeast as a model for the study of cytokinesis

et al. 2011

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Cytokinesis is the final stage of the cell cycle, and ensures completion of both genome segregation and organelle distribution to the daughter cells. Cytokinesis requires the cell to solve a spatial problem (to divide in the correct place, orthogonally to the plane of chromosome segregation) and a temporal problem (to coordinate cytokinesis with mitosis). Defects in the spatiotemporal control of cytokinesis may cause cell death, or increase the risk of tumor formation [Fujiwara et al., 2005 (Fujiwara T, Bandi M, Nitta M, Ivanova EV, Bronson RT, Pellman D. 2005. Cytokinesis failure generating tetraploids promotes tumorigenesis in p53-null cells. Nature 437:1043–1047); reviewed by Ganem et al., 2007 (Ganem NJ, Storchova Z, Pellman D. 2007. Tetraploidy, aneuploidy and cancer. Curr Opin Genet Dev 17:157–162.)]. Asymmetric cytokinesis, which permits the generation of two daughter cells that differ in their shape, size and properties, is important both during development, and for cellular homeostasis in multicellular organisms [reviewed by Li, 2007 (Li R. 2007. Cytokinesis in development and disease: variations on a common theme. Cell Mol Life Sci 64:3044–3058)]. The principal focus of this review will be the mechanisms of cytokinesis in the mitotic cycle of the yeast Schizosaccharomyces pombe. This simple model has contributed significantly to our understanding of how the cell cycle is regulated, and serves as an excellent model for studying aspects of cytokinesis. Here we will discuss the state of our knowledge of how the contractile ring is assembled and disassembled, how it contracts, and what we know of the regulatory mechanisms that control these events and assure their coordination with chromosome segregation. © 2011 Wiley-Liss, Inc.

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Polar opposites: Fine‐tuning cytokinesis through SIN asymmetry

2012

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Mitotic exit and cell division must be spatially and temporally integrated to facilitate equal division of genetic material between daughter cells. In the fission yeast, Schizosaccharomyces pombe, a spindle pole body (SPB) localized signaling cascade termed the septation initiation network (SIN) couples mitotic exit with cytokinesis. The SIN is controlled at many levels to ensure that cytokinesis is executed once per cell cycle and only after cells segregate their DNA. An interesting facet of the SIN is that its activity is asymmetric on the two SPBs during anaphase; however, how and why the SIN is asymmetric has remained elusive. Many key factors controlling SIN asymmetry have now been identified, shedding light on the significance of SIN asymmetry in regulating cytokinesis. In this review, we highlight recent advances in our understanding of SIN regulation, with an emphasis on how SIN asymmetry is achieved and how this aspect of SIN regulation fine‐tunes cytokinesis. © 2012 Wiley Periodicals, Inc

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Etd1p is a novel protein that links the SIN cascade with cytokinesis

Cited by 29 publications

References 50 publications

Antagonistic Roles of PP2A-Pab1 and Etd1 in the Control of Cytokinesis in Fission Yeast

Antagonistic Roles of PP2A-Pab1 and Etd1 in the Control of Cytokinesis in Fission Yeast

Dividing the spoils of growth and the cell cycle: The fission yeast as a model for the study of cytokinesis

Polar opposites: Fine‐tuning cytokinesis through SIN asymmetry

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