Établissement d'une cryobanque  d'embryons pour la conservation ex situ  de la diversité génétique chez le lapin :  aspects pratiques

Joly, Thierry; Rochambeau, Hubert de; Renard, Jean‐Paul

doi:10.1051/gse:19980715

Cited by 5 publications

(9 citation statements)

References 2 publications

(2 reference statements)

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“…This variability can be due to numerous factors such as the genetic of the embryos, the genetic of the does, the housing conditions, the feeding conditions… In this study, we used the pregnancy rate and the live-foetuses rate that can be compared to the parturition rate and the live-born rate respectively. The pregnancy rate obtained with the CRYO3 group was similar to the parturition rates obtained in other studies [49], [51]. In the same way, the live-foetuses rate was similar to the lowest live-born rates obtained in other studies [45], [51].…”

Section: Discussionsupporting

confidence: 88%

“…Thus, in the literature, the parturition rate (number of doe giving at least one live-born pup/total recipient does) can range from 59% [49] to 100% [44] and the live-born rate (number of living pups/number of embryos transferred) can range from 19% [45] to 55% [50]. This variability can be due to numerous factors such as the genetic of the embryos, the genetic of the does, the housing conditions, the feeding conditions… In this study, we used the pregnancy rate and the live-foetuses rate that can be compared to the parturition rate and the live-born rate respectively.…”

Section: Discussionmentioning

confidence: 99%

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A Chemically Defined Medium for Rabbit Embryo Cryopreservation

Bruyère¹,

Baudot²,

Joly³

et al. 2013

PLoS ONE

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This study evaluates a new synthetic substitute (CRYO3, Ref. 5617, Stem Alpha, France) for animal-based products in rabbit embryo cryopreservation solutions. This evaluation was performed using two approaches: a thermodynamic approach using differential scanning calorimetry and a biological approach using rabbit embryo slow-freezing. During the experiment, foetal calf serum (FCS) was used as a reference. Because FCS varies widely by supplier, three different FCS were selected for the thermodynamic approach. The rabbit embryo slow-freezing solutions were made from Dulbecco's phosphate buffer saline containing 1.5 M Dimethyl Sulfoxide and 18% (v.v−1) of CRYO3 or 18% (v.v−1) of FCS. These solutions were evaluated using four characteristics: the end of melting temperature, the enthalpy of crystallisation (thermodynamic approach) and the embryo survival rates after culture and embryo transfer (biological approach). In the thermodynamic approach, the solutions containing one of the three different FCS had similar mean thermodynamic characteristics but had different variabilities in the overall data with aberrant values. The solution containing CRYO3 had similar thermodynamic properties when compared to those containing FCS. Moreover, no aberrant value was measured in the solution containing CRYO3. This solution appears to be more stable than the solutions containing a FCS. In the biological approach, the in vitro embryo survival rates obtained with the solution containing CRYO3 (73.7% and 81.3%) and with the solution containing a FCS (77.6% and 71.9%) were similar (p = 0.7). Nevertheless, during the in vivo evaluation, the implantation rate (21.8%) and the live-foetuses rate (18.8%) of the CRYO3 group were significantly higher than the implantation rate (7.1%, p = 0.0002) and the live-foetuses rate (5.3%, p = 0.0002) of the FCS group. The pregnancy rate was also higher in the CRYO3 group compared to the FCS group (81.3% and 43.8%, respectively, p = 0.066). We conclude that CRYO3 can be used as a chemically defined substitute for animal-based products in rabbit embryo cryopreservation solutions.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

A Chemically Defined Medium for Rabbit Embryo Cryopreservation

Bruyère¹,

Baudot²,

Joly³

et al. 2013

PLoS ONE

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“…In the studies by Joly [22], it was demonstrated that an effective preservation of characteristics such as growth rate and litter size could be obtained with the offspring of 9 males from different reproductive groups (9 fathers) for a heritability of these characters of 0.25 and 0.10 and variation coefficients of 0.10 and 0.35, respectively. In accordance with the number of different males and does with offspring after cryopreservation, the values of the effective population size (Ne) for maternal line (V) were higher than 70 and for growth line (R) about 46, so it was possible to re-establish the lines with an increment of consanguinity coefficient around 1% and it was possible to assess the genetic gain of the selection process.…”

Section: Discussionmentioning

confidence: 99%

Effect of rabbit line on a program of cryopreserved embryos by vitrification

et al. 2003

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-The aim of this study was to assess the application of a cryopreservation program to preserve two selected rabbit lines. One of them was selected by litter size at weaning, line V (Synthetic breed). The second, line R (synthetic breed), was selected by growth rate. In this study, embryos were collected, from donor does belonging to the 7th and 15th generations of lines R and V, respectively, were vitrified and were stored from 1992-1993. Those embryos from donor does belonging to the 17th and 21st generations of lines R and V respectively, were vitrified and sotred from 1998-1999. Embryo transfers were carried out in 1999. Morphologically normal embryos at the morulae stage were cryopreserved by vitrification in a 2.8 M dimethyl-sulfoxide + 3.5 M ethyleneglycol + 0.3 g·L -1 bovine serum albumine in Dulbecco phosphate buffered saline solution. The main problem in the cryopreservation program was the low embryo production efficiency: significant differences were obtained in recovery efficiency between lines and line R showed the lowest proportion of donor does with 55% (at least 4 normal embryos) vs. 72% in line V. However, after transfer in recipient does of line V, the fertility rate at birth (81%), the rate of alive born by pregnant recipients (43%) and the number of males and does with offspring (9 to 18 different males, 12 to 32 females) enabled the different generations from each line to be re-established and studies on the selection process genetic gain to be developed. cryopreservation / embryo / line / rabbit

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“…Su alto contenido en ácido siálico provoca su larga permanencia en sangre (Maertens and Luzi, 1995); esta característica permite su administración en una única dosis y, por tanto, minimizar la manipulación de los animales. En cambio, el contenido en ácido siálico de la FSH es menor y, por tanto, presenta una vida media corta ; de este modo, el protocolo de administración más frecuentemente utilizado ha sido el de la administración de dosis repetidas cada 12 horas durante 3 o 4 días (Kennelly and Foote, 1965;Joly, 1997;Besenfelder, 2002;Salvetti et al, 2007b).…”

Section: Hormonas Y Pautas De Tratamientounclassified

“…A este respecto hay diferentes posibilidades: crioconservación de semen, de embriones, de ovocitos e incluso la conservación de tejidos más complejos como, por ejemplo, el tejido ovárico. El hecho de que la mayor parte de programas de conservación se basen en la conservación de semen y embriones podría deberse a causas como la facilidad en la formación de un banco de semen (Boettcher et al, 2005) o la inmediatez en la obtención de descendencia en el caso de los embriones, ya que tan sólo hace falta la transferencia de los mismos a hembras receptoras para su obtención (Joly, 1997). Sin embargo, se ha de tener en cuenta que, en caso de tener que recuperar una población, podría llevarse a cabo tan sólo con un banco de semen realizando retrocruzamientos, si bien habría que asumir el hecho de que nunca podría recuperarse el 100% de la genética original (Boettcher et al, 2005); de ahí que sea aconsejable combinar, al menos, dos tipos de muestras en la formación de un banco (Boettcher et al, 2005).…”

Section: Introducción _______________________________________________unclassified

Efecto De La Aplicación De Gonadotropinas Recombinantes Humanas Sobre La Producción Y Calidad De Ovocitos Y Embriones en Coneja

Nicolau¹

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Établissement d'une cryobanque d'embryons pour la conservation ex situ de la diversité génétique chez le lapin : aspects pratiques

Cited by 5 publications

References 2 publications

A Chemically Defined Medium for Rabbit Embryo Cryopreservation

A Chemically Defined Medium for Rabbit Embryo Cryopreservation

Effect of rabbit line on a program of cryopreserved embryos by vitrification

Efecto De La Aplicación De Gonadotropinas Recombinantes Humanas Sobre La Producción Y Calidad De Ovocitos Y Embriones en Coneja

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