Estudo morfológico e molecular da mama de ratas castradas tratadas com isoflavona ou estrogênios

Piovesan, Ana Claudia; Júnior, José Maria Soares; Mosquette, Rejane; Simões, Manuel de Jesus; Simões, Ricardo; Baracat, Edmund Chada

doi:10.1590/s0100-72032005000400007

Cited by 13 publications

(10 citation statements)

References 18 publications

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“…The work was approved by the Committee of Ethics in Research of the College of Medicine ⁄ Antô nio Pedro Universitary Hospital ⁄ Fluminense Federal University. Anaesthesia was intramuscular with 100 mg ⁄ kg of ketamine (Crystal) and 20 mg ⁄ kg of xylazine (Anasedan Vetbrands) (Piovesan et al 2005).…”

Section: Ovariectomymentioning

confidence: 99%

Effect of prolonged use of high dose of tibolone on the vagina of ovariectomized rats

Henriques

Carvalho

Filho

et al. 2011

International Journal of Experimental Pathology

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The aim of this study was evaluate the effect of prolonged use of high dose of tibolone on the vagina of ovariectomized rats. Bilateral ovariectomy was performed on 14 rats weighing 250 g. Thirty days later, vaginal smears were collected verifying the menopause status by anoestrus cytology. Rats were divided randomly into groups: experimental rats (n = 9) received 1 mg tibolone/day orally and control rats (n = 6) received placebo (carboxymethylcellulose). After 150 days, all rats were sedated and euthanized by cervical displacement. The vagina was removed, fixed in 10% buffered formalin, sampled and processed for paraffin embedding. Histological sections were stained with haematoxylin and eosin, picrosirius red, periodic acid Schiff (PAS) and PAS-diastase, and Weigert's resorcin-fuchsin. Cell proliferation was analysed by immunohistochemistry to detect Ki67. Histomorphometric analyses were performed for epithelial thickness, per cent area of collagen fibres and blood vessels, mast cells and Ki67-positive nuclei per mm of basal membrane. Means and standard error of means were calculated, and data were compared using the Mann-Whitney test, with significance level at P < 0.05. In the vagina, epithelial thickness, number of Ki67-positive nuclei per mm of basal membrane, number of vessels and number of mast cells were significantly higher in the tibolone group when compared with the control group. Furthermore, the content of glycogen and glycoproteins in the vaginal epithelium was modified by tibolone. Tibolone administered in high dose and for a long period has a trophic effect, reversing vaginal atrophy, and has no dysplastic or neoplastic effect in the vagina of ovariectomized rats.

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Section: Ovariectomymentioning

confidence: 99%

Effect of prolonged use of high dose of tibolone on the vagina of ovariectomized rats

Henriques

Carvalho

Filho

et al. 2011

International Journal of Experimental Pathology

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“…Receptor activation also results in increased expression of EGFR (epidermal growth factor receptor) and synthesis of mRNA, facilitating a higher rate of tumor growth [54]. Estrogens also activate VEGF-A (vascular endothelial growth factor) which is related to the process of angiogenesis and closely related with proliferative processes [55].…”

Section: Molecular Mechanism Of Hrt Carcinogenmentioning

confidence: 99%

The Risk of Relationship between Hormonal Replacement Therapy and Cancer

Rosales¹

2014

CRJ

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Abstract:Menopause is an endocrine condition due to the decline in ovarian activity, which occurs in all women and is characterized as a progressive hypoestrogenism. To minimize damage from lack hormonal activity, hormone replacement therapy has been developed (TRH), which is widely used around the world, often without criteria pre-established. Thus, this study aims to identify hormonal therapies used postmenopausal and relate them to the development of tumors. In addition, identify the criteria for the use of HRT, the utilization time and women who are part of the risk groups are also objectives of this study. In the beginning, the clinical methodology of HRT included only estrogen, but epidemiological studies have observed that the progestin presence is essential in endometrial hyperplasia control adding to the therapy. Progestogens act depending on its molecular structure, and consequently selectivity, or may interact with other steroid receptors. From this interaction, different pharmacodynamic answers about importance in the development of tumors can be observed, ie, progesterone activity, estrogen, antiestrogen, androgen and / or antiandrogen. In view of the mechanism of carcinogen administration of estrogens, it can be said that HRT increases the risk of breast, uterus and ovary cancer. However, the estrogenic actions may be adjusted when there is administration of progestin drugs and with antiestrogenic and antiandrogenic actions. Given this, in menopausal women, it is advisable to trace gynecological cancer, before, during and after any therapeutic interventions, and the decision of using and the choice of HRT on an individual basis, through a careful assessment of signs and symptoms and long-term risk.

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“…A anestesia foi intramuscular com quetamina (100 mg/kg) e xilazina (20 mg/kg) (24) . Após 30 dias da ooforectomia, o status de menopausa cirúrgica foi confirmado mediante avaliação hormonal pela citologia vaginal (3,25) , em esfregaços corados pela técnica de Papanicolau.…”

Section: Procedimento Cirúrgicounclassified

Avaliação do glicogênio hepático correlacionado com glicose sérica em ratas castradas sob tratamento com tibolona

Filho¹,

Kanaan²,

Guzmán-Silva³

2011

J. Bras. Patol. Med. Lab.

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Introdução: O glicogênio representa a forma de armazenamento de açúcares na célula animal, sendo estocada naturalmente no hepatócito. Em sua dinâmica metabólica ocorre participação de receptores, hormônios e enzimas que mantêm e equilibram os níveis séricos desse componente. Objetivo: O presente estudo investigou a influência da tibolona no metabolismo glicídico hepático por meio de avaliação da presença do glicogênio hepático e níveis séricos de glicose. Material e métodos: Utilizamos ao todo 14 ratas Wistar, em menopausa cirúrgica comprovada citologicamente, tratadas diariamente com tibolona (n = 9) ou com placebo (n = 5) durante 20 semanas. Efetuou-se avaliação dos pesos do animal e do fígado e dos níveis de glicose sérica. O estudo morfológico foi realizado em cortes histológicos de tecido hepático, corados com hematoxilina e eosina (HE) e com ácido periódico de Schiff (PAS), com e sem amilase salivar. Para avaliação do glicogênio no fígado, utilizou-se grade de estudo morfológico (GEM), que delineia as regiões metabólicas e circulatórias do lóbulo hepático. Resultados: O peso dos animais foi menor no Grupo Tibolona, com glicose sérica em níveis mais baixos; já o peso relativo do fígado foi significativamente maior (p < 0,001). No Grupo Controle o glicogênio apresentou distribuição heterogênea em três diferentes padrões e o Grupo Tibolona mostrou glicogênio uniforme em toda a estrutura lobular. Conclusão: A tibolona, administrada em alta dose e por tempo prolongado, determina perda de peso por deficiência alimentar, que leva a alterações nas funções hepáticas, podendo influir na glicogenólise e na gliconeogênese, com modificações do glicogênio hepático e da glicose circulante.

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Estudo morfológico e molecular da mama de ratas castradas tratadas com isoflavona ou estrogênios

Cited by 13 publications

References 18 publications

Effect of prolonged use of high dose of tibolone on the vagina of ovariectomized rats

Effect of prolonged use of high dose of tibolone on the vagina of ovariectomized rats

The Risk of Relationship between Hormonal Replacement Therapy and Cancer

Avaliação do glicogênio hepático correlacionado com glicose sérica em ratas castradas sob tratamento com tibolona

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