Estudo experimental comparativo do enxerto homólogo pulmonar tratado pelo processo L-Hydro com o homoenxerto pulmonar a fresco

Rey, N; Moreira, Luíz Felipe Pinho; Cheung, David T.; Casagrande, Ivan S. J.; Benvenuti, Luiz Alberto; Stolf, Noedir Antônio Groppo

doi:10.1590/s0102-76382011000200020

Cited by 2 publications

(3 citation statements)

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“…Rey et al [24] in their experimental study with homografts preserved in L-Hydro R found from the optical microscopy, slightly retracted cusps with progressive thinning toward the free edge. The atomic absorption spectroscopy, as well as macro and microscopic evaluations, showed higher concentrations of calcium in the prosthetic Glutaraldehyde Group.…”

Section: Discussionmentioning

confidence: 94%

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Replacement of pulmonary artery trunk in sheep using tubular valved heterograft in non-aldehydic preservation

Souza¹,

Palma²,

Casagrande³

et al. 2012

Revista Brasileira De Cirurgia Cardiovascular

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Substituição do tronco da artéria pulmonar em carneiros utilizando heteroenxerto tubular valvado com preservação não-aldeídicaReplacement of pulmonary artery trunk in sheep using tubular valved heterograft in non-aldehydic preservation 8 Abstract Introduction: The cardiac bioprostheses are related to thromboembolic events, infectious and degenerative diseases. Wear is mainly attributed to the denaturation of collagen. Glutaraldehyde, the predominant method of preservation of bioprostheses, favors the calcification process and limits their durability. Several techniques try to contain the degenerative process of bioprostheses.Objectives: To evaluate the process of calcification in vivo pulmonary valve heterografts preserved in non-aldehydic (L-Hydro®).Methods: Seventeen sheep underwent replacement of the pulmonary artery valved tubular grafts of bovine pericardium. The animals were divided into two groups: Group L-Hydro® (test / n = 14) and Group Glutaraldehyde (control / n = 3). About 150 days after implantation the animals were sacrificed, necropsied and implants subjected to a pathological study, radiological evaluation and measurement of calcium by atomic absorption spectrophotometry. Statistical analysis was obtained through the Fisher's exact test, Student's t or Mann-Whitney test (significance: 5%).Results: The radiological evaluation, the macroscopic and microscopic measurement of serum calcium by atomic absorption spectrophotometry showed increased calcification of the prosthetic group Glutaraldehyde, when compared to 420 Souza HJB, et al. -Replacement of pulmonary artery trunk in sheep using tubular valved heterograft in non-aldehydic preservation Rev Bras Cir Cardiovasc 2012;27(3):419-28denture-HydroR Group L (P = 0.001). Seven animals in Group L-Hydro® (50%) had adherence of the leaflets to the wall of the tube (P = 0.228).Conclusions: Prostheses preserved in L-Hydro® were more resistant to calcification when compared with glutaraldehyde preserved.Descriptors: Bioprosthesis. Glutaral. Polyethylene glycols. Heart valve prosthesis. Heart valve diseases/surgery.

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Section: Discussionmentioning

confidence: 94%

“…By scanning electron microscopy and transmission electron microscopy, Rey et al [24] showed histological evidence of interstitial and endothelial repopulation on the surface of homografts preserved in L-Hydro R implanted in sheep. PEG is attributed to the immunosuppressive property, which is based on L-Hydro R preservation.…”

Section: Discussionmentioning

confidence: 99%

Replacement of pulmonary artery trunk in sheep using tubular valved heterograft in non-aldehydic preservation

Souza¹,

Palma²,

Casagrande³

et al. 2012

Revista Brasileira De Cirurgia Cardiovascular

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“…The juvenile ovine model was selected for testing the valve in vivo because of the similarity of the animal's anatomy and hemodynamics to humans (46,47) and because the model is approved by both the FDA and CE Mark (48). The exact definition of what constitutes a juvenile sheep is lacking, and in line with others (49)(50)(51), we elected to use animals that were 6 months of age. This negates the excessive growth of younger animals while still providing an accelerated calcification model for the valves.…”

Section: Experimental Animalsmentioning

confidence: 99%

Development and testing of a transcatheter heart valve with reduced calcification potential

Weich,

Botes,

Doubell

et al. 2023

Front. Cardiovasc. Med.

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IntroductionPatients from developing countries who require heart valve surgery are younger and have less access to open heart surgery than those from developed countries. Transcatheter heart valves (THVs) may be an alternative but are currently unsuitable for young patients because of their inadequate durability. We developed and tested a THV utilizing two new types of decellularized bovine pericardial leaflets in an ovine model.MethodsThe two decellularized tissues [one with a very low dose (0.05%) of monomeric glutaraldehyde (GA) fixation and detoxification (DF) and the other without glutaraldehyde (DE)] were compared to an industry standard [Glycar—fixed with the standard dose (0.625%) of glutaraldehyde]. THVs were manufactured with the three tissue types and implanted in the pulmonary position of nine juvenile sheep for 180 days. Baseline and post-explantation evaluations were performed to determine the hemodynamic performance of the valves and their dynamic strength, structure, biological interaction, and calcification.ResultsHeart failure occurred in one animal due to incompetence of its Glycar valve, and the animal was euthanized at 158 days. The gradients over the Glycar valves were higher at the explant than at the implant, but the DE and DF valves maintained normal hemodynamic performance throughout the study. The DF and DE tissues performed well during the mechanical testing of explanted leaflets. Glycar tissue developed thick pannus and calcification. Compared to Glycar, the DF tissue exhibited reduced pannus overgrowth and calcification and the DE tissue exhibited no pannus formation and calcification. All tissues were endothelialized adequately. There was a striking absence of host ingrowth in the DE tissue leaflets, yet these leaflets maintained integrity and mechanical function.ConclusionIn the juvenile sheep THV model, Glycar tissue developed significant pannus, calcification, and hemodynamic deterioration. Using a very low dose of monomeric GA to fix the decellularized bovine pericardium yielded less pannus formation, less calcification, and better hemodynamic function. We postulate that the limited pannus formation in the DF group results from GA. Bovine pericardium decellularized with our proprietary method resulted in inert tissue, which is a unique finding. These results justify further development and evaluation of the two decellularized tissue types in THVs for use in younger patients.

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Estudo experimental comparativo do enxerto homólogo pulmonar tratado pelo processo L-Hydro com o homoenxerto pulmonar a fresco

Cited by 2 publications

References 25 publications

Replacement of pulmonary artery trunk in sheep using tubular valved heterograft in non-aldehydic preservation

Replacement of pulmonary artery trunk in sheep using tubular valved heterograft in non-aldehydic preservation

Development and testing of a transcatheter heart valve with reduced calcification potential

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