Estudo dos fatores que condicionam acidez elevada em leite humano: aspectos microbiológicos e nutricionais

Abstract

The study of the factors that condition high acidity in human milk: microbiological and nutritional aspects Seventy two samples of human breastmilk obtained from the Milk of the Hospitals Group of Sorocaba were analyzed, prior to pasteurization. Dornic acidity was titrated in duplicate for each sample. Forty eight of them had acceptable acidity (up to 8° D) and other 24 samples with acidity above of that limit. Quantifying the initial population: lactic bacteria, lipolitics bacteria, total coliforms and Escherichia coli, aerobic mesofilic microorganisms and Staphylococcus positive coagulase. Forty nine questionnaires of alimentary frequency were applied in lactic donors of milk with the purpose of to detect correlation among microbiology, acidity and nutrition of the lactic ones. The samples with value of acidity <8°D the found populations of microorganism were: group of the microorganisms mesofilic total aerobic and lactic bacteria the found maximum population was 10 6 CFU/mL. Although for lipolitics bacteria this value did not cross 1,9x10 5 CFU/mL. The group of total coliforms were isolated in 47,2% (34 samples) of the analyzed samples, of these 87,5% they also confirmed the presence of E.coli. In Staphylococcus positive coagulase the detect maximum value was 1x104 CFU/mL and it was similar to the acid samples and no acid. In the samples with acidity above 8ºD (rejected) the maximum population so much for the mesofilic group as of lactic bacteria they were 10 7 CFU/mL. The lipolitics bacteria were found in 53,52% (13 samples) of the total of analyzed samples and the group of the coliforms was detected in 52,94% of the acid samples, of these 33,33% they presented growth of E. coli. Data were analyzed to detect correlation between variables: population of aerobic mesofilic microorganisms and acidity Dornic, using Pearson`s coefficient. The level of significance was set at p <0,05. The values of acidity Dornic did not show correlation (R = 0,215) statistically significant with the population of aerobic mesofilic microorganisms (CFU/mL) for the analyzed samples. According to the data nutritional, small differences are noticed regarding to the consumption of foods and alteration of acidity of the human milk. For the found values it was not possible to detect correlation between the nutrition of the lactic and the values of acidity found in the samples of human milk.

Leite humano como fonte de bactérias lácticas produtoras de bacteriocinas e com potencial probiótico

“…Segundo Heine (1992), citado por Scarso (2008) Klebsiela, Enterobacter, Serratia (SERAFINI et al, 2003) e bactérias do ácido láctico ou lácticas, que se destacam no alimento em questão.…”