Estudo da composição química, toxicidade e atividade moluscicida do óleo essencial Citrus sinensis (L.) Osbeck

Abstract

Determinamos a composição química e testamos a toxicidade e a atividade moluscicida do óleo essencial extraído das cascas do Citrus sinensis (L.) Osbeck. Para isso, o óleo essencial foi extraído quantitativamente por hidrodestilação. Em seguida, as quantificações de seus componentes foram realizadas por cromatografia gasosa acoplada à espectrometria de massas (CG-MS) e a toxicidade e atividade moluscicida foram testadas, respectivamente, contra Artemia salina e caramujos Biomphalaria glabrata. Os resultados mostraram que o óleo contém 81,50% de D-limoneno (constituinte principal) e 0,06% de citronelal (componente menor) e este possui atividade moluscicida com concentração letal (CL50) de 100,08 mg.L-1 e atoxicidade, com CL50 de 321,84 mg.L-1 a um nível de confiança de 95%. Portanto, o óleo é ativo contra o caramujo Biomphalaria glabrata e atóxico para outros seres vivos.

Chemical composition and larvicidal activity of essential oil of the bark of Citrus sinensis (L.) Osbeck

“…In the first found, from the chromatographic analysis coupled to the mass spectrometer of the oil distilled from the shells C. sinensis (L.) Osbeck we identified 15 compounds in which limonene was present in greater quantity. The result of our study differs somewhat from previous studies, as in these the percentage of limonene ranged from 71.3 to 98% [18,[25][26][27]. Generally, we attribute this difference to the interference of some factors such as: seasonality, temperature, collection period, intensity of solar radiation, age, and development of plants, among others [28,29].…”

“…Sample collection took place similarly to the study by Gomes et al [18], however with some modifications. We collected the samples (fruits), branches and leaves of the plant species Citrus sinensis (L.) Osbeck on the campus of the Federal Institute of Education, Science and Technology of Maranhão (IFMA) -Maracanã, located at Avenida dos Curiós, S / Nº, Vila Esperança, whose exsiccates presented identification number 01484 at "Herbário Ático Seabra" of the Universidade Federal do Maranhão.…”

“…We identified the constituents of the essential oil by gas chromatography coupled with mass spectrometry (GC-MS) equipped with the same configurations as the study by Gomes et al [18]. The volatile constituents were observed in a Shimadzu gas chromatograph, coupled to a GC-MS mass spectrometer QP5050A, equipped with a BPX 5% phenylpolysphenylene-siloxane capillary column, (30 m length x 0.25 mm thick and 0.…”

“…Among citrus species with high limonene content, we highlight Citrus sinensis (L.) Osbeck. In the literature, there are reports that the oil distilled from this plant has molluscicidal [18], antifungal [19], antimicrobial, antiaflatoxigenic and antioxidant activity [20].…”

Efektivitas Larvasida Kombinasi Daun Suren (Toona sureni) dan Jeruk Nipis (Citrus aurantiifolia)

“…Kandungan senyawa kimia yang terkandung dalam daun suren dan daun jeruk nipis sebagai larvasida adalah flavonoid, saponin, tanin, triterpenoid, limonoid/limonen yang dapat menyebabkan kematian larva. Kandungan paling banyak adalah limonen sebesar 81,69% (Gomes et al, 2021;Isfanda et al, 2023;Mangampa et al, 2018;Sheng et al, 2020;Sousa et al, 2023).…”