Estudo comparativo dos métodos coprológicos de Lutz, Kato-Katz e Faust modificado

Chaves, A. S.; Alcantara, O. S.; Carvalho, Omar dos Santos; Santos, José Soares dos

doi:10.1590/s0034-89101979000400010

Cited by 20 publications

(15 citation statements)

References 5 publications

(4 reference statements)

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“…In addition, the extensive use of BM is not recommended because of its low positivity rates for other parasites. However, when combined with the modified Faust method, the BM technique can be advantageously employed in epidemiological inquiries and in routine laboratory stool examinations for the detection of enteric helminths (Chaves et al 1979).…”

Section: Discussionmentioning

confidence: 99%

A comparative study of the TF-Test®, Kato-Katz, Hoffman-Pons-Janer, Willis and Baermann-Moraes coprologic methods for the detection of human parasitosis

Carvalho

Moreira

Pena

et al. 2012

Mem. Inst. Oswaldo Cruz

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Section: Discussionmentioning

confidence: 99%

A comparative study of the TF-Test®, Kato-Katz, Hoffman-Pons-Janer, Willis and Baermann-Moraes coprologic methods for the detection of human parasitosis

Carvalho

Moreira

Pena

et al. 2012

Mem. Inst. Oswaldo Cruz

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“…The fecal material of each mollusk collected was processed following the methodology of spontaneous sedimentation (Chaves et al, 1979), using feces that were homogenized with water and transferred to a 100 ml settling cup through a specific filter, for further microscopic analysis.…”

Section: Biometry Analysis Of Mucus and Fecal Materialsmentioning

confidence: 99%

Achatina fulica (Gastropoda: Pulmonata): Occurrence, environmental aspects and presence of nematodes in Sergipe, Brazil

et al. 2020

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Abstract The objective of this study was to identify the occurrence of Achatina fulica in the Great Aracaju-SE Territory. This research was developed during the months of June 2016 to May 2017. The plots methodology was used for the monthly collections, following the collection time of 20 minutes per area. The mollusks were measured and soon afterwards the parasitological analysis of the mucus and faeces was performed. Molecular identification was performed to evaluate the possible presence of Angiostrongylus sp. We observed that the occurrence of A. fulica was more representative in urban areas, especially in vacant lots with the presence of litter and decomposing materials. In the specimens of A. fulica examined were male and female nematodes of the genus Rhabditis, in the research municipalities, except in Barra dos Coqueiros. No parasites of the genus Angiostrongylus were found in the mollusks examined. The influence of the presence of litter on the plots associated with the frequency of A. fulica was statistically significant. There was a correlation between the influence of rainfall and the frequency of A. fulica. A significant relationship between moisture and frequency and nematode positivity was identified. It was also observed that the larger the mollusk, the greater the chances of being infected by nematodes. Scientific knowledge about the biology of the A. fulica species associated with human intervention such as the maintenance of land and adequate sanitary measures may be important factors for a better control of this invasive mollusk.

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“…They were all examined for intestinal parasites using coproparasitoscopic studies (for multiple ova and parasites); we used the method of Faust with subsequent staining with Lugol solution in order to find cysts (19,20). We used E. histolytica DNA as a positive control, which was obtained from a monogenic culture of strain HM-1 IMSS donated by the Molecular Diagnostic Laboratory, Department of Experimental Pathology CINVESTAV.…”

Section: Methodsmentioning

confidence: 99%

Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis

López-López

Martínez-López

Boldo-León

et al. 2017

Braz J Med Biol Res

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Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differentiate between Entamoeba histolytica (pathogenic) and E. dispar (non-pathogenic). The target for the PCR amplification was a small region (228 bp) of the adh112 gene selected to increase the sensitivity of the test. The study involved 62 stool samples that were collected from individuals with complaints of gastrointestinal discomfort. Of the 62 samples, 10 (16.1%) were positive for E. histolytica while 52 (83.9%) were negative. No sample was positive for E. dispar. These results were validated by nested PCR-RFLP (restriction fragment length polymorphism) and suggest that PCR-DGGE is a promising tool to differentiate among Entamoeba infections, contributing to determine the specific treatment for patients infected with E. histolytica, and therefore, avoiding unnecessary treatment of patients infected with the non-pathogenic E. dispar.

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Estudo comparativo dos métodos coprológicos de Lutz, Kato-Katz e Faust modificado

Cited by 20 publications

References 5 publications

A comparative study of the TF-Test®, Kato-Katz, Hoffman-Pons-Janer, Willis and Baermann-Moraes coprologic methods for the detection of human parasitosis

A comparative study of the TF-Test®, Kato-Katz, Hoffman-Pons-Janer, Willis and Baermann-Moraes coprologic methods for the detection of human parasitosis

Achatina fulica (Gastropoda: Pulmonata): Occurrence, environmental aspects and presence of nematodes in Sergipe, Brazil

Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis

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