ESTUDIOS CITOGENÉTICOS EN LA TORTUGA PINTADA (Trachemys dorbigni: TESTUDINATA, EMYDIDAE)

Salas, Anabel Eliana; Imhof, Alba; Amavet, Patricia Susana

doi:10.14409/natura.v1i45.4355

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2019

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“…The presence of a single heterochromatic NOR-bearing pair (chromosomes 14) also confirms previous reports [De Smet, 1978;Bickham and Rogers, 1985;Cleiton and Giuliano-Caetano, 2008;Martinez et al, 2009;Salas et al, 2014;Badenhorst et al, 2015;Montiel et al, 2016]. We detected no interstitial telomeric sequences in E. orbicularis as in previous reports on Trachemys scripta and T. dorbigni [Martinez et al .…”

Section: Discussionsupporting

confidence: 92%

Insights into Emydid Turtle Cytogenetics: The European Pond Turtle as a Model Species

Iannucci

Svartman

Bellavita³

et al. 2019

Cytogenet Genome Res

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Our knowledge of Testudines evolution is limited by the lack of modern cytogenetic data. Compared to other reptiles, there is little information even on chromosome banding, let alone molecular cytogenetic data. Here, we provide detailed information on the karyotype of the European pond turtle Emys orbicularis, a model Emydidae, employing both chromosome banding and molecular cytogenetics. We provide a high-resolution G-banded karyotype and a map of rDNA genes and telomeric sequences using fluorescence in situ hybridization. We test hypotheses of sex-determining mechanisms in Emys by comparative genomic hybridization to determine if Emys has a cryptic sex-specific region. Our results provide valuable data to guide future efforts on genome sequencing and anchoring in Emydidae and for understanding karyotype evolution in Testudines.

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