“…The optimal concentration for each stage of the process ranges from 2 to 3%, with the exception of cormel formation, where a higher concentration of sucrose is advised (Supplementary Table S1). For better in vitro results, some researchers have adjusted media using sucrose in various percentages, including 2% [35,82,83] and 3% [9,11,27,28,[33][34][35]38,39,54,58,59,63,70,81,83,84,[86][87][88][89][90]107]. Various researchers have reported varying and higher concentrations of sugar, up to 5% [31], 7% [29], 12% [108], and 15% sucrose [109], as well as different ranges of sucrose, like 2-12% [22], 3-8% [64], 3-9% [67], and 3-12% [110].…”