Estudio Citoquimico De Las Principales Enzimas Hidroliticas Del<i>Paracoccidioides brasiliensis</i>

Albornoz, María B. de; Aasen, Imelda Campo

doi:10.1080/00362177185190301

Cited by 6 publications

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“…Requirement of amino acids and sulfur and their possible relation to dimorphism (Paris et al, 1985;Cano and Aguiar, 1991) as well as some of its protease activities are known (Albornoz and Campo-Aasen, 1971;Mendez-Giannini et al, 1990;Bedoya-Escobar et al, 1993;Vaz et al, 1994); and the nutritional requirements of P. brasiliensis have been compared with those of Blastomyces dermatitidis Gilchrist et Stokes (Gilardi and Laffer, 1962). DNA patterns of different isolates of P. brasiliensis by RAPD have been analyzed and it was suggested that the studied isolates could be separated in two different groups (Soares et al, 1995).…”

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Characteristics of 17 Paracoccidioides brasiliensis isolates

et al. 1997

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We have studied the physiological and morphological features of 17 isolates of Paracoccidioides brasiliensis in order to define their phenotypes. The isolates were cultured at room temperature on potato dextrose agar (PDA, Difco) slants for mycelial growth and in 1% dextrose brain heart infusion agar (BHIA, Difco) at 37~ for the study of yeast forms. Most mycelial and yeast forms grew well between pH 5.6-9.4. In their response to osmotic pressure the isolates were separated in three groups: intolerant, intermediate and tolerant. They also varied in carbohydrate assimilation tests, which indicated important metabolic variation. No clear differences were observed in phenol oxidase tests, KNO3, starch, casein and arbutin assimilation tests. Only 1 of the isolates, Bt-19, had gelatinase activity. No correlation was observed between the above differences and virulence. Two patterns of growth were observed in the mycelial cultures, glabrous and cottonous, the latter being correlated with increased virulence for ddY mice. Most yeast forms grew as cerebriform colonies, but Pb-HC and Bt-19 colonies had a cobblestone-like surface.

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confidence: 99%