Estrus synchronization affects WNT signaling in the porcine reproductive tract and embryos

Kiewisz, Jolanta; Kaczmarek, Monika M.; Morawska, E.; Blitek, Agnieszka; Kapelański, W.; Ziȩcik, Adam J.

doi:10.1016/j.theriogenology.2011.06.034

Cited by 13 publications

(21 citation statements)

References 37 publications

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“…Besides changes in WNT gene expression in endometrial, conceptus and luteal tissue, we detected decreased concentrations of P4 in the blood serum on days 9 and 16 of pregnancy in PGF2 α /eCG/hCG‐treated gilts compared with control animals (Kiewisz et al. ). As the number of CLs was greater in synchronized than in non‐synchronized gilts (Kiewisz et al.…”

Section: Discussionmentioning

confidence: 86%

Effect of Oestrus Synchronization with PGF2α/eCG/hCG on Luteal P4 Synthesis in Early Pregnant Gilts

Szymańska

Morawska-Pucinska

Krawczynski

et al. 2014

Reprod Domestic Animals

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Administration of hormones to synchronize oestrus is a useful tool in animal breeding. However, exogenous ovarian stimulation may be detrimental to reproductive function. This study was aimed to examine whether an oestrus synchronization with PGF2α/eCG/hCG could affect luteal P4 synthesis in early pregnant gilts. Corpora lutea (CLs) were collected on days 9, 12 and 16 of pregnancy from gilts with natural (n = 16) and synchronized (n = 18) oestrus and analysed for (i) the expre-ssion of steroidogenic acute regulatory protein (StAR), cytochrome P450 family 11 subfamily A polypeptide (CYP11A1), and 3β-hydroxysteroid dehydrogenase (3βHSD); (ii) the concentration of P4 in the luteal tissue and blood; and (iii) the expression of luteinizing hormone receptors (LHR) and oestrogen receptors (ERα and ERβ). Additionally, the effect of LH on P4 secretion from CL slices collected from synchronized and naturally ovulated animals has been studied in vitro. PGF2α /eCG/hCG administration increased mRNA expression of StAR, CYP11A1, 3βHSD, and LHR on day 9 and CYP11A1 and LHR on day 12 of pregnancy compared with the control group (p < 0.05). CYP11A1, 3βHSD, LHR, ERα and ERβ proteins were not affected by synchronization; only StAR protein increased in hormonally treated animals (p = 0.017). The concentration of P4 in luteal tissue was greater on day 9 (p < 0.01), but lower on day 16 (p < 0.05) in gilts with hormonally induced oestrus compared with control animals. Blood serum levels of P4 were lower in synchronized than control gilts (p < 0.001). Synchronization did not affect LH-stimulated P4 secretion from luteal slices; however, greater basal concentration of P4 in incubation medium was detected for CLs collected from synchronized than control gilts (p < 0.05). In conclusion, synchronization of oestrus with PGF2α/eCG/hCG protocol in gilts did not impair the expression of luteal P4 synthesis system, although decreased P4 concentration in the blood.

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Section: Discussionmentioning

confidence: 86%

Effect of Oestrus Synchronization with PGF2α/eCG/hCG on Luteal P4 Synthesis in Early Pregnant Gilts

Szymańska

Morawska-Pucinska

Krawczynski

et al. 2014

Reprod Domestic Animals

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“…In adult rodent granulosa cells Wnt4 is elevated in response to hCG stimulation and remains elevated in the corpora lutea (Hsieh et al 2002). Likewise, estrus synchronization of gilts utilizing PGF2α/PMSG/hCG increased expression of WNT4 in luteal tissue compared to control females (Kiewisz et al 2011). Targeted deletion of Wnt4 in mouse granulosa cells resulted in subfertile females with smaller ovaries and fewer healthy antral follicles at 42-d of age compared with control mice (Boyer et al 2010).…”

Section: The Role Of Wnt In Follicle Developmentmentioning

confidence: 93%

The role of WNT signaling in adult ovarian folliculogenesis

Gifford

2015

Reproduction

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Wingless-type mouse mammary tumor virus integration site (WNT) signaling molecules are locally secreted glycoproteins that play a role in a number of physiological and pathological developmental processes. Components of the WNT signaling pathway have been demonstrated to impact reproductive functions including embryonic development of the sex organs, and regulation of follicle maturation controlling steroidogenesis in the postnatal ovary. Emerging evidence underscores the complexity of WNT signaling molecules in regulation of dynamic changes that occur in the ovary during the reproductive cycle. While disruption in the WNT signaling cascade has been recognized to have deleterious consequences to normal sexual development, more recent studies are beginning to highlight the importance of these molecules in adult ovarian function related to follicle development, corpus luteum formation, steroid production and fertility. Hormonal regulation of WNT genes and expression of members of the WNT signaling network, including WNT ligands, frizzled receptors and downstream signaling components that are expressed in the postnatal ovary at distinct stages of the estrous cycle, suggest a crucial role in normal ovarian function. Similarly, FSH stimulation of T cell factor-dependent gene expression requires input from β-catenin, a lynchpin molecule in canonical WNT signaling, further indicating β-catenin participation in regulation of follicle maturation. This review will focus on the multiple functions of WNT signaling in folliculogenesis in the adult ovary.

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“…During porcine estrus cycle and pregnancy, gene expression levels of Wnt5a showed variation during pregnancy and corresponding day of estrus cycle and Wnt7a was differentially expressed at different stages of estrus cycle and early pregnancy, though Wnt4 and β-catenin gene expressions were maintained at the constant level in the endometrium of cyclic and pregnant pigs [34]. However content of estrogen (E2) in the uterine luminal flushings as well as blood serum level of progesterone (P4) in cyclic gilts of pigs showed no statistically significant correlations with Wnt4, Wnt5a, Wnt7a, β-catenin and Cdh1 gene expression.…”

Section: Wnt Signaling In Uterine Receptivitymentioning

confidence: 97%

“…Differentiating Wnt gene expression profiles at different stages of estrus cycle and pregnancy were identified in different species [34,35]. During porcine estrus cycle and pregnancy, gene expression levels of Wnt5a showed variation during pregnancy and corresponding day of estrus cycle and Wnt7a was differentially expressed at different stages of estrus cycle and early pregnancy, though Wnt4 and β-catenin gene expressions were maintained at the constant level in the endometrium of cyclic and pregnant pigs [34].…”

Section: Wnt Signaling In Uterine Receptivitymentioning

confidence: 99%

“…However content of estrogen (E2) in the uterine luminal flushings as well as blood serum level of progesterone (P4) in cyclic gilts of pigs showed no statistically significant correlations with Wnt4, Wnt5a, Wnt7a, β-catenin and Cdh1 gene expression. Moreover, treatment of endometrial tissue explants with different concentrations of E2 or P4 added separately or simultaneously had no effect on Wnt5a, Wnt7a, β-catenin and Cdh1 gene expression [34]. Diethylstilbestrol (DES) exposure suppressed the expression of Wnt4, Wnt5a, Wnt7a, Wnt11, Wnt16, and FZD10 in the neonatal mouse uterus [24].…”

Section: Wnt Signaling In Uterine Receptivitymentioning

confidence: 99%

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The role of Wnt signaling members in the uterus and embryo during pre-implantation and implantation

Tepekoy

Akkoyunlu

Demir

2014

J Assist Reprod Genet

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Wnt family members are best known for their roles in cell fate determination, differentiation, proliferation and apoptosis during embryonic development. Wnt signaling becomes effective during these cellular processes through the proper interaction between its ligands, receptors, effectors and inhibitors. Here we review Wnt signaling in terms of embryonic development to the blastocyst stage implantation with emphasis on endometrial changes that are critical for receptivity in the uterus. The relationship between Wnt signaling and implantation clearly reveals that, Wnt family members are critical for both early embryonic development and changing of the endometrium before implantation. Specific Wnt signaling pathway members are demonstrated to be critical for endometrial events such as decidualization and endometrial gland formation in addition to cyclic changes in the endometrium controlled by reproductive hormones. In conclusion, specific roles of Wnt members and associated factors for both uterine function and embryonic development should be further investigated with respect to the efficiency of human ARTs.

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Estrus synchronization affects WNT signaling in the porcine reproductive tract and embryos

Cited by 13 publications

References 37 publications

Effect of Oestrus Synchronization with PGF2α/eCG/hCG on Luteal P4 Synthesis in Early Pregnant Gilts

Effect of Oestrus Synchronization with PGF2α/eCG/hCG on Luteal P4 Synthesis in Early Pregnant Gilts

The role of WNT signaling in adult ovarian folliculogenesis

The role of Wnt signaling members in the uterus and embryo during pre-implantation and implantation

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