Estrogenicity profile and estrogenic compounds determined in river sediments by chemical analysis, ELISA and yeast assays

Assessing estrogenic activity in surface water and sediment of the Liao River system in northeast China using combined chemical and biological tools

“…But the measured EEQ values for the water samples from site 11 and site 19 and for the sediment samples from site 9 and site 15 were higher than those calculated values (Table 3). This suggests that some unknown chemicals might contribute to the estrogenicity of the samples (Soto et al, 2004;Sarmah et al, 2006;Viganò et al, 2008). The present study demonstrates that estrogenic activity in a sample may not be fully explained by chemical analysis of the selected target compounds, and an estrogenic screening bioassay such as the YES assay could provide the overall estrogenicity of a sample.…”

“…E-SCREEN, yeast estrogen screen (YES) and estrogen responsive chemically activated luciferase (ER-CALUX)) based on the interaction between chemicals and estrogenic receptors can be used to evaluate the integrated estrogenic activity of environmental samples, without the necessity of knowing all compounds present that contribute to the activity (Soto et al, 1995;Routledge and Sumpter, 1996;Legler et al, 2002). Combined in vitro bioassay and chemical analysis could be used to better assess causal links between effects observed in the aquatic environment and chemical analysis results (Sumpter and Johnson, 2008;Viganò et al, 2008;Streck, 2009). However, more research is needed for the assessment of estrogenicity in aquatic environments by using the combined chemical and biological tools.…”

Monitoring of selected estrogenic compounds and estrogenic activity in surface water and sediment of the Yellow River in China using combined chemical and biological tools

“…This suggests that some unknown chemicals might contribute to the estrogenicity of the samples (Sarmah et al, 2006;Soto et al, 2004;Viganò et al, 2008).…”

Estrogenic activity profiles and risks in surface waters and sediments of the Pearl River system in South China assessed by chemical analysis and in vitro bioassay

“…In parallel with chemical analysis, in vivo or in vitro bioassays are often used to screen the estrogenic potency of the environmental samples. [15][16][17] Screening using bioassays could provide an integral estrogenic activity of a sample, and could also help evaluate the estrogenic contributions by certain chemicals in combination with chemical analysis. Surface water and sediments in rivers of Europe, the USA, Japan and Korea had displayed various degrees of estrogenic activities due to the presence of estrogenic substances.…”