Estrogenicity determination in carp, Cyprinus carpio: a laboratory and field approach

Solé, Montserrat; Castillo, Montserrat; Alda, María J. López de; Porte, Cinta; Barceló, D.

doi:10.1051/analusis:2000280783

Cited by 6 publications

(6 citation statements)

References 24 publications

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“…Currently, the estrogenic effects of EDCs are well known, and a widely accepted measure of estrogenicity is the determination of VTG (Solé et al 2000). The results of the present research clearly show that the VTG concentration was higher in those organisms exposed to E2 in all age groups and exposure pathways.…”

Section: Discussionsupporting

confidence: 56%

“…Among the diversity of estrogenic compounds identified in the environment, E2, estrone (natural hormones) and 17a-ethinylestradiol (EE2; synthetic estrogen used in birth control pills) are compounds considered responsible for the major estrogenic effects in wild organisms (Duong et al 2010). Steroids are mostly excreted in a less active conjugated form; however, their deconjugation by microorganisms originates a more potent parent compound (Solé et al 2000). Although it is known that E2 and EE2 are the main estrogens stimulating hepatic VTG synthesis in teleosts, the molecular mechanism of estrogen action in phylogenetically ancient fish, remains poorly studied.…”

Section: Discussionmentioning

confidence: 99%

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Physiological response of alligator gar juveniles (Atractosteus spatula) exposed to sub-lethal doses of pollutants

González

Cruz

Alfaro

2015

Fish Physiol Biochem

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Alligator gar populations have declined because of overfishing, habitat loss and pollution. Over time, the exposure to different pollutants have affected these fishes as a consequence of their high trophic level, bottom-dwelling habits and long life span. In order to evaluate the physiological effects of pollutants on alligator gar, juveniles (6, 12 and 24 months) were exposed to sub-lethal doses of diazinon, β-naphthoflavone (BNF) and 17 β-estradiol (E2) by intraperitoneal injection. After 2 days of exposure, liver samples were taken to determine the activities of acetylcholinesterase, butyrylcholinesterase and carboxylesterase; alkaline and acid phosphatases (ALP and ACP); ethoxyresorufin o-deethylase (EROD); glutathione s-transferase (GST); superoxide dismutase (SOD), and vitellogenin (VTG) concentration. Two additional bioassays consisting on the exposure of compounds through water or food were performed and after 4 and 28 days, respectively, biomarkers were determined. All esterases were inhibited in organisms exposed to diazinon as well as in 6-months gar exposed to E2 and BNF. In contrast, ALP activity increased in gar exposed to diazinon and E2, while ACP activity did not show any variations. No EROD activity was registered after exposure to the different pollutants, despite being one of the most sensitive and common detoxification biomarkers used for fishes. GST activity reduction was detected when gar were exposed to E2 and BNF, while SOD activity increased after exposure to diazinon and E2. Finally, VTG levels were higher in animals exposed to E2 compared to other treatments. Overall, these results suggest that alligator gar juveniles have a low biotransformation metabolism and show that they are especially sensitive to those pollutants affecting the nervous system.

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Physiological response of alligator gar juveniles (Atractosteus spatula) exposed to sub-lethal doses of pollutants

González

Cruz

Alfaro

2015

Fish Physiol Biochem

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“…Most of the European in situ investigations on this topic have been carried out with endemic species such as roach (Rutilis rutilis) [2,[5][6][7], gudgeon (Gobio gobio) [8], bream (Abramis brama) [9][10][11][12][13], and carp [14][15][16][17] or with caged rainbow trout (Oncorhynchus mykiss) [18]. The endemic species used in field surveys in Europe all tend to have their natural habitat in the lower parts of the river systems.…”

Section: Introductionmentioning

confidence: 99%

Vitellogenin as a biomarker for estrogenic effects in brown trout, Salmo trutta: Laboratory and field investigations

Bjerregaard¹,

Hansen²,

Larsen³

et al. 2008

Enviro Toxic and Chemistry

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The sensitivity of juvenile brown trout towards estrogenic chemicals (17beta-estradiol [E2], estrone [E1], 17alpha-ethinylestradiol [EE2], 4-tert-octylphenol [OP], and n-butylparaben [BP]) was tested in laboratory experiments with plasma and liver vitellogenin concentrations as endpoints. Vitellogenin concentrations were also assessed in juvenile brown trout collected in streams affected by agricultural runoff and discharges from scattered houses in the open land. In the laboratory, juvenile brown trout were exposed to the chemicals in flow-through tanks for 7 to 12 d and concentration-response relationships for the induction of vitellogenin synthesis were obtained. The actual exposure concentrations were determined by liquid chromatography-mass spectrometry. The median plasma vitellogenin concentration in first year control brown trout reared in recirculated groundwater was 165 ng/ml with 783 ng/ml as the highest value. The median effective concentration (EC50) values for vitellogenin induction (based on plasma concentrations) were 3.7 ng EE2/L, 15 ng E2/L, 88 ng E1/L, 68 microg BP/L, and 7 microg OP/L. Median effective concentrations derived from liver vitellogenin concentrations were similar. The 166 brown trout caught in the field were mainly first and second year fish and a few third year fish. Plasma vitellogenin concentrations were below 1000 ng/L in 146 of the fish, between 1000 ng/L and 4234 ng/L in 19 fish and 5.3 x 10(6) ng/L in one male fish. Vitellogenin concentrations did not differ between first and second year fish, but were elevated in third year fish. The data may indicate that juvenile (<2 years) trout with plasma vitellogenin concentrations above 1000 ng/ml have had their vitellogenin synthesis induced by exposure to estrogens in the environment. Plasma and liver vitellogenin concentrations were closely correlated in brown trout with elevated vitellogenin concentrations. It is noteworthy, however, that exposure to synthetic estrogens (EE2, BP, and OP) resulted in higher liver concentrations (for the same plasma concentration) than exposure to the natural estrogens E1 and E2.

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“…The latter indicates that the fish were exposed to an unidentified exogenous estrogenic chemical (4). Previous research has revealed increased concentrations of VTG in the blood of carp (4)(5)(6)(7)(8)(9)(10) and other cyprinids (11,12) exposed to municipal wastewater treatment plant (WWTP) effluent in enclosures or captured from riverine sites influenced by WWTP effluent. Estrogenic chemicals found in municipal WWTP effluent and implicated as potential causative agents for VTG induction in male fish exposed to these effluents include the animal steroids E2 and estrone, the oral contraceptive medication component ethinylestradiol (EE2), and the alkylphenol polyethoxylates (APE) and their degradation products, the alkylphenols (AP) (5,(13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Reproductive Responses of Common Carp (Cyprinus carpio) Exposed in Cages to Influent of the Las Vegas Wash in Lake Mead, Nevada, from Late Winter to Early Spring

Snyder

Kelly

et al. 2004

Environ. Sci. Technol.

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The Las Vegas Wash (LW) delivers tertiary-treated municipal wastewater effluent, nonpotable shallow groundwater seepage, and runoff from the urbanized Las Vegas Valley to Las Vegas Bay (LX) of Lake Mead. To investigate the potential for contaminants in LW influent to produce effects indicative of endocrine disruption in vivo, adult male and female common carp (Cyprinus carpio) were exposed in cages for 42-48 d at four sites in Lake Mead: LW, LX, and two reference locations in the lake. End points examined included gonadosomatic index; gonad histology; concentrations of plasma vitellogenin (VTG) and plasma sex steroids (17beta-estradiol (E2), testosterone (T), 11-ketotestosterone (11-KT)); plasma estrogen:androgen ratios (E2:T, E2:11-KT), in vitro production of T by gonad tissue, and hepatopancreas ethoxyresorufin O-deethylase activity. Few differences among fish caged at different sites were potentially attributable to exposure to contaminants

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Estrogenicity determination in carp, Cyprinus carpio: a laboratory and field approach

Cited by 6 publications

References 24 publications

Physiological response of alligator gar juveniles (Atractosteus spatula) exposed to sub-lethal doses of pollutants

Physiological response of alligator gar juveniles (Atractosteus spatula) exposed to sub-lethal doses of pollutants

Vitellogenin as a biomarker for estrogenic effects in brown trout, Salmo trutta: Laboratory and field investigations

Reproductive Responses of Common Carp (Cyprinus carpio) Exposed in Cages to Influent of the Las Vegas Wash in Lake Mead, Nevada, from Late Winter to Early Spring

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