Estrogenic endpoints in fish early life‐stage tests: Luciferase and vitellogenin induction in estrogen‐responsive transgenic zebrafish

Bogers, Rinus; Mutsaerds, Esther; Druke, Jeanette; Roode, Daphne F. de; Murk, Albertinka J.; Burg, Bart van der; Legler, Juliette

doi:10.1897/05-234r.1

Cited by 33 publications

(19 citation statements)

References 33 publications

(50 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bogers et al (2006) found no negative influence on mortality, development and length growth of transgenic zebrafish (Danio rerio) in the early life stages when exposed over However, a significant positive effect on weight growth was observed (35% compared to the control). Although not significantly different, a 12% higher mean weight of fish exposed to the lowest concentration (0.2 ml·l -1 ) of DMSO, compared to the control group was found in the current study.…”

Section: Discussionmentioning

confidence: 79%

Early Ontogeny, Growth and Mortality of Common Carp (Cyprinus carpio) at Low Concentrations of Dimethyl Sulfoxide

et al. 2009

View full text Add to dashboard Cite

Dimethyl sulphoxide (DMSO) is an important polar aprotic solvent, less toxic than other members of this class. Because of its excellent solvating power, DMSO is frequently used as solvent for chemical reactions involving salts, especially Finkelstein reactions and other nucleophilic substitutions. Furthermore, DMSO is used as auxiliary substance in toxicity tests on aquatic organisms, usually at concentrations of 0.2 and 0.5 ml·l -1 . The aim of the present study was to evaluate the effect of DMSO on early development, growth and mortality of early life stages in common carp. Tests were performed from fertilized egg to the end of the larval period with continuous exposure to DMSO at concentrations of 0.2, 0.5, 1.0 and 5.0 ml·l -1 . Mortality of embryos and larvae, intensity of development and growth, weight and occurrence of abnormalities were analyzed. DMSO exposure at all the tested concentrations did not result in any lethal effects or abnormalities compared to the control over the entire test period (29 days). Growth indicators were similar in DMSO treated (at concentrations of 0.2, 0.5 and 1.0 ml·l ) and control groups. However, mean body weight (35 ± 9 mg) of larvae exposed to the highest DMSO concentration (5 ml·l -1 ) tested was significantly lower compared to the control (45 ± 14 mg). DMSO, auxiliary substance, toxicity, fish embryos and larvaeAquatic toxicology tests are designed for readily water soluble substances. However, due to the occurrence of several water-insoluble substances, the use of various solvents is needed. A major concern in toxicology studies is the choice of appropriate solvents. Carrier solvents may also have toxic effects on organisms (Hallare et al. 2006;Ball 1966;Rayburn and Fisher 1997;Benville 1968). Hence, it was necessary to set guidelines that will describe the types and the maximum allowable concentrations of the solvent to be used in experimental systems that will cause no significant impact on the observed results (Hallare et al. 2006).Dimethyl sulphoxide (DMSO) is commonly used in fish toxicity tests as a solvent for several water-insoluble substances (Rufli et al. 1998;Rayburn et al. 1991). DMSO is a colourless hygroscopic liquid that is synthesized by the oxidation of dimethyl sulphide, or originates as a waste product from wood pulp processing. Its chemical structure creates extraordinary properties. The sulphur-oxygen bond determines the polar nature of DMSO with the capability to dissolve sparingly soluble substances, and it is completely miscible in water, common organic solvents and unsaturated hydrocarbons (Willhite and Katz 1984). Furthermore, the chemical structure of DMSO enables it to penetrate membrane barriers by increasing the permeability without affecting the morphologic characteristics of the skin and body membranes. Therefore, DMSO acts as a skin penetration enhancer for drugs and other substances (Kligman 1965a; Kligman 1965b;Jacob et al. 1964;Stoughton and Fritsch 1964). The main applications and properties of DMSO were summarized in reviews...

show abstract

Section: Discussionmentioning

confidence: 79%

Early Ontogeny, Growth and Mortality of Common Carp (Cyprinus carpio) at Low Concentrations of Dimethyl Sulfoxide

et al. 2009

View full text Add to dashboard Cite

show abstract

“…This assay has been performed using adult zebrafish and juveniles (30 dpf). The latter, which are at the stage of gonad differentiation, appear to be more responsive (Legler et al 2000, Legler et al 2002, Bogers et al 2006. In addition, two transgenic zebrafish lines have been generated in which GFP is used, enabling microscopic analysis of the reporter activity.…”

Section: Figurementioning

confidence: 99%

Nuclear receptor research in zebrafish

Schaaf

2017

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

Nuclear receptors (NRs) form a superfamily of transcription factors that can be activated by ligands and are involved in a wide range of physiological processes. NRs are well conserved between vertebrate species. The zebrafish, an increasingly popular animal model system, contains a total of 73 NR genes, and orthologues of almost all human NRs are present. In this review article, an overview is presented of NR research in which the zebrafish has been used as a model. Research is described on the three most studied zebrafish NRs: the estrogen receptors (ERs), retinoic acid receptors (RARs) and peroxisome proliferator-activated receptors (PPARs). The studies on these receptors illustrate the versatility of the zebrafish as a model for ecotoxicological, developmental and biomedical research. Although the use of the zebrafish in NR research is still relatively limited, it is expected that in the next decade the full potential of this animal model will be exploited.

show abstract

“…In these lines, an Er-responsive promoter is genetically fused to a reporter gene encoding either luciferase or GFP. Using a transgenic line with the luciferase gene coupled to a promoter with a single estrogen response element (ERE), Er activity could be measured after 96 hour exposure to estrogens by measuring the luciferase activity in tissue homogenates [17,30]. This assay has been performed using adult zebrafish and juveniles, in which adults appear to be more responsive.…”

Section: The Zebrafish Ersmentioning

confidence: 99%

The First Fifteen Years of Steroid Receptor Research in Zebrafish; Characterization and Functional Analysis of the Receptors

Schaaf¹

2017

Nuclear Receptor Research

View full text Add to dashboard Cite

Abstract. Steroid hormones regulate a wide range of processes in our body, and their effects are mediated by steroid receptors. In addition to their physiological role, these receptors mediate the effects of endocrine disrupting chemicals (EDCs) and are widely used targets for dugs involved in the treatment of numerous diseases, ranging from cancer to inflammatory disorders. Over the last fifteen years, the zebrafish has increasingly been used as an animal model in steroid receptor research. Orthologues of all human steroid receptor genes appear to be present in zebrafish. All zebrafish steroid receptors have been characterized in detail, and their expression patterns have been analyzed. Functional studies have been performed using morpholino knockdown of receptor expression and zebrafish lines carrying mutations in one of their steroid receptor genes. To investigate the activity of the receptors in vivo, specific zebrafish reporter lines have been developed, and transcriptomic studies have been carried out to identify biomarkers for steroid receptor action. In this review, an overview of research on steroid receptors in zebrafish is presented, and it is concluded that further exploitation of the possibilities of the zebrafish model system will contribute significantly to the advancement of steroid receptor research in the next decade.

show abstract

Estrogenic endpoints in fish early life‐stage tests: Luciferase and vitellogenin induction in estrogen‐responsive transgenic zebrafish

Cited by 33 publications

References 33 publications

Early Ontogeny, Growth and Mortality of Common Carp (Cyprinus carpio) at Low Concentrations of Dimethyl Sulfoxide

Early Ontogeny, Growth and Mortality of Common Carp (Cyprinus carpio) at Low Concentrations of Dimethyl Sulfoxide

Nuclear receptor research in zebrafish

The First Fifteen Years of Steroid Receptor Research in Zebrafish; Characterization and Functional Analysis of the Receptors

Contact Info

Product

Resources

About