Estrogenic activity of wastewater, bottled waters and tap water in Finland as assessed by a yeast bio-reporter assay

Omoruyi, Iyekhoetin Matthew; Pohjanvirta, Raimo

doi:10.1177/1403494815591686

Cited by 10 publications

(10 citation statements)

References 29 publications

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“…These observations corroborate that the proposed treatment strategy did not originate compounds with estrogenic effects. Similar results were obtained when applying recombinant yeasts expressing estrogen receptor alpha to evaluate the estrogenic activity of wastewater, bottled waters and tap water in Finnish samples (Omoruyi and Pohjanvirta, 2015). For LDH assay, cellular viability was maintained after treatment for both Caco-2 and hCMEC/D3 cells, providing values similar to those obtained for culture media.…”

Section: Biological Effectssupporting

confidence: 73%

Photocatalytic ozonation of urban wastewater and surface water using immobilized TiO2 with LEDs: Micropollutants, antibiotic resistance genes and estrogenic activity

et al. 2016

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Photocatalytic ozonation was employed for the first time in continuous mode with TiO2-coated glass Raschig rings and light emitting diodes (LEDs) to treat urban wastewater as well as surface water collected from the supply area of a drinking water treatment plant (DWTP). Different levels of contamination and types of contaminants were considered in this work, including chemical priority substances (PSs) and contaminants of emerging concern (CECs), as well as potential human opportunistic antibiotic resistant bacteria and their genes (ARB&ARG). Photocatalytic ozonation was more effective than single ozonation (or even than TiO2 catalytic ozonation) in the degradation of typical reaction by-products (such as oxalic acid), and more effective than photocatalysis to remove the parent micropollutants determined in urban wastewater. In fact, only fluoxetine, clarithromycin, erythromycin and 17-alpha-ethinylestradiol (EE2) were detected after photocatalytic ozonation, by using solid-phase extraction (SPE) pre-concentration and LC-MS/MS analysis. In surface water, this treatment allowed the removal of all determined micropollutants to levels below the limit of detection (0.01-0.20 ng L(-1)). The efficiency of this process was then assessed based on the capacity to remove different groups of cultivable microorganisms and housekeeping (16S rRNA) and antibiotic resistance or related genes (intI1, blaTEM, qnrS, sul1). Photocatalytic ozonation was observed to efficiently remove microorganisms and ARGs. Although after storage total heterotrophic and ARB (to ciprofloxacin, gentamicin, meropenem), fungi, and the genes 16S rRNA and intI1, increased to values close to the pre-treatment levels, the ARGs (blaTEM, qnrS and sul1) were reduced to levels below/close to the quantification limit even after 3-days storage of treated surface water or wastewater. Yeast estrogen screen (YES), thiazolyl blue tetrazolium reduction (MTT) and lactate dehydrogenase (LDH) assays were also performed before and after photocatalytic ozonation to evaluate the potential estrogenic activity, the cellular metabolic activity and the cell viability. Compounds with estrogenic effects and significant differences concerning cell viability were not observed in any case. A slight cytotoxicity was only detected for Caco-2 and hCMEC/D3 cell lines after treatment of the urban wastewater, but not for L929 fibroblasts.

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Section: Biological Effectssupporting

confidence: 73%

Photocatalytic ozonation of urban wastewater and surface water using immobilized TiO2 with LEDs: Micropollutants, antibiotic resistance genes and estrogenic activity

et al. 2016

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“…(Ma et al 2005 ; Mahjoub et al 2009 ; Dagnino et al 2010 ; Macova et al 2010 ; Reungoat et al 2010 ; Mahjoub et al 2011 ). The estradiol EQs observed for water samples in this study was in the range 0.4–0.9 ng/L, which is lower or comparable to other studies of waste water treatment plant outlets (Dagnino et al 2010 ; Omoruyi and Pohjanvirta 2015 ; Välitalo et al 2017 ). The detected toxic responses show that this experimental design might be useful when using bioanalytical tools for water quality assessments of environmental water samples, especially for water samples from contaminated areas.…”

Section: Discussionsupporting

confidence: 77%

Toxicity bioassays with concentrated cell culture media—a methodology to overcome the chemical loss by conventional preparation of water samples

Niss

Rosenmai

Mandava

et al. 2018

Environ Sci Pollut Res

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The use of in vitro bioassays for studies of toxic activity in environmental water samples is a rapidly expanding field of research. Cell-based bioassays can assess the total toxicity exerted by a water sample, regardless whether the toxicity is caused by a known or unknown agent or by a complex mixture of different agents. When using bioassays for environmental water samples, it is often necessary to concentrate the water samples before applying the sample. Commonly, water samples are concentrated 10–50 times. However, there is always a risk of losing compounds in the sample in such sample preparation. We have developed an alternative experimental design by preparing a concentrated cell culture medium which was then diluted in the environmental water sample to compose the final cell culture media for the in vitro assays. Water samples from five Swedish waste water treatment plants were analyzed for oxidative stress response, estrogen receptor (ER), and aryl hydrocarbon receptor (AhR) activity using this experimental design. We were able to detect responses equivalent to 8.8–11.3 ng/L TCCD for AhR activity and 0.4–0.9 ng/L 17β-estradiol for ER activity. We were unable to detect oxidative stress response in any of the studied water samples. In conclusion, we have developed an experimental design allowing us to examine environmental water samples in toxicity in vitro assays at a concentration factor close to 1, without the risk of losing known or unknown compounds during an extraction procedure.Electronic supplementary materialThe online version of this article (10.1007/s11356-018-1656-4) contains supplementary material, which is available to authorized users.

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“…Although the YES bioassay has several advantages, including its robustness, lack of endogenous receptors that can interfere with the response of the cells, lower cost and uncomplicated bioassay procedure, yeast-based bioassays are less sensitive compared to human cell lines (Connolly et al, 2011;Kunz et al, 2015). Estrogenic activity was also below the detection limit in drinking water from Brazil (Bergamasco et al, 2011) and Finland (Omoruyi and Pohjanvirta, 2015) using yeast based assays. The YES bioassay is therefore not as suitable for detection of low levels of estrogenic activity normally found in drinking water.…”

Section: Bioassays For Estrogenic Activitymentioning

confidence: 99%

Estrogenic activity, chemical levels and health risk assessment of municipal distribution point water from Pretoria and Cape Town, South Africa

et al. 2017

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Endocrine disrupting chemicals (EDCs) are ubiquitous in the environment and have been detected in drinking water from various countries. Although various water treatment processes can remove EDCs, chemicals can also migrate from pipes that transport water and contaminate drinking water. This study investigated the estrogenic activity in drinking water from various distribution points in Pretoria (City of Tshwane) (n = 40) and Cape Town (n = 40), South Africa, using the recombinant yeast estrogen screen (YES) and the T47D-KBluc reporter gene assay. The samples were collected seasonally over four sampling periods. The samples were also analysed for bisphenol A (BPA), nonylphenol (NP), di(2-ethylhexyl) adipate (DEHA), dibutyl phthalate (DBP), di(2-ethylhexyl) phthalate (DEHP), diisononylphthalate (DINP), 17β-estradiol (E), estrone (E) and ethynylestradiol (EE) using ultra-performance liquid chromatography-tandem mass spectrophotometry (UPLC-MS/MS). This was followed by a scenario based health risk assessment to assess the carcinogenic and toxic human health risks associated with the consumption of distribution point water. None of the water extracts from the distribution points were above the detection limit in the YES bioassay, but the EEq values ranged from 0.002 to 0.114 ng/L using the T47D-KBluc bioassay. BPA, DEHA, DBP, DEHP, DINP E, E and EE were detected in distribution point water samples. NP was below the detection limit for all the samples. The estrogenic activity and levels of target chemicals were comparable to the levels found in other countries. Overall the health risk assessment revealed acceptable health and carcinogenic risks associated with the consumption of distribution point water.

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Estrogenic activity of wastewater, bottled waters and tap water in Finland as assessed by a yeast bio-reporter assay

Cited by 10 publications

References 29 publications

Photocatalytic ozonation of urban wastewater and surface water using immobilized TiO2 with LEDs: Micropollutants, antibiotic resistance genes and estrogenic activity

Photocatalytic ozonation of urban wastewater and surface water using immobilized TiO2 with LEDs: Micropollutants, antibiotic resistance genes and estrogenic activity

Toxicity bioassays with concentrated cell culture media—a methodology to overcome the chemical loss by conventional preparation of water samples

Estrogenic activity, chemical levels and health risk assessment of municipal distribution point water from Pretoria and Cape Town, South Africa

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