Estrogenic activity of environmental polycyclic aromatic hydrocarbons in uterus of immature Wistar rats

Kummer, V.; Mašková, J.; Zralý, Z.; Neča, Jiřı́; Šimečková, Pavlína; Vondráček, Jan; Machala, Miroslav

doi:10.1016/j.toxlet.2008.06.862

Cited by 83 publications

(39 citation statements)

References 73 publications

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“…The increased uterus and pituitary weights and hydrometra in the rats treated with DES from day 0 of pregnancy were thought to be due to the strong estrogenicity of DES [7,9,12]. The bred rats with neutrophil infiltration in the endometrium and vaginal keratosis were thought to be in pro-estrus or estrus [13].…”

Section: Discussionmentioning

confidence: 99%

Effects of Exposure Period and Dose of Diethylstilbestrol on Pregnancy in Rats

Kawaguchi

Miyoshi

Miyamoto

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. The aim of this study was to investigate the effect of exposure period and dose of diethylstilbestrol (DES), which has strong estrogenic activity, on pregnancy in rats. All rats with observed vaginal plugs or sperm in vaginal smear tests after mating were divided into 3 groups: those fed a normal diet, a diet mixed with DES throughout pregnancy and a diet mixed with DES from day 13 of pregnancy. DES was mixed into the diet at 0.1, 1, 10 and 100 ppm. All bred rats fed the normal diet and 0.1 ppm DES from day 13 of pregnancy delivered pups, while none of the rats treated with 1-100 ppm DES during pregnancy and 100 ppm DES from day 13 of pregnancy delivered any pups. The number of male and female pups born decreased in rats treated with 10 ppm DES from day 13 of pregnancy. These results suggest that DES could affect pregnancy and that the exposure period and dose may result in sterility, abortion, poor fetal growth and reduced number of pups born.KEY WORDS: diethylstilbestrol (DES), lactation, pregnancy, rat.J. Vet. Med. Sci. 71 (10): [1309][1310][1311][1312][1313][1314][1315] 2009 There is evidence that many environmental endocrine disruptors act as sex hormones, particularly during the perinatal or neonatal period, directly or indirectly affecting reproduction in human and rats [14,21,23]. Endogenous hormone mimics have been proposed for a number of adverse human health effects, including infertility, abnormal prenatal and childhood development and reproductive cancers [18].In humans, women exposed to diethylstilbestrol (DES), a synthetic estrogen with strong estrogenic activity, during pregnancy have a moderately increased risk of breast cancer [17,23]. It is also known that the frequency of vaginal clear cell adenocarcinoma is increased in young women whose mothers used DES to prevent or lower the risk of abortion [4,23]; women had taken the DES tablets at 50-200 mg/day during pregnancy [1]. However, DES is ineffective in prevention of miscarriages and premature births [4,23]. DES has also been used as a good model reagent for investigation pre-or post-natal effects of estrogenic compounds on females using animal models for human extrapolation.We have previously reported that neonatal administration of DES induced persistent estrus and an absence of corpus lutea in female rats due to disturbance of the gonadotropinsecreting system in the hypothalamus [11,16,24]. However, it remains to be determined whether the administration of DES affects pregnancy in rats.In this study, doses of 0.1 to 100 ppm DES were selected because daily gavages with DES at 90 mg/kg/day during pregnancy result in high prenatal fetal mortality in rats [2]. The period after major organogenesis also selected for examination of DES, from day 13 of pregnancy, because the period of major organogenesis in rats is from day 6 to day 15 of pregnancy [8]; in particular, development of mammary hillocks occurs on day 13 of pregnancy [20]. Therefore, the pregnant rats were fed a diet containing 0.1 to 100 ppm DES during the entire pregnanc...

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Section: Discussionmentioning

confidence: 99%

Effects of Exposure Period and Dose of Diethylstilbestrol on Pregnancy in Rats

Kawaguchi

Miyoshi

Miyamoto

et al. 2009

The Journal of Veterinary Medical Science

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“…AhR agonists are also reported to inhibit various growth factors and modulate crosstalk between AhR and ERα pathways (Safe, 1999), and to increase proteosomal degradation of the ER receptor (Wormke et al, 2003) (Ohtake et al, 2011). On the other hand, there are conflicting reports indicating that the hydroxylated metabolites of B[a]P are estrogenic in vitro (Fertuck et al, 2001) and both B[a]P and B [a]A are estrogenic in the immature rat uterotrophic assay after three days of sc (Kummer et al, 2008). The discrepancy might be related to the difference between the immature and adult OVX assay, known to have different hepatic metabolism and/or to the length of the exposure periods (3 days versus 7 days).…”

Section: Discussionmentioning

confidence: 99%

Ovariectomized mouse uterotrophic assay of 36 chemicals

Ohta

Takagi²,

Ohmukai

et al. 2012

J. Toxicol. Sci.

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“…Previous studies have confirmed that most PAHs are potential environmental endocrine disruptors and they are carcinogenic and/or mutagenic (Grimmer, 1983;Elovaara et al, 2007;Smith et al, 2007). Animal studies found that some PAHs affected the function of estrogen and were related to infertility (Kummer et al, 2008;Smith et al, 2007). In addition, studies have shown that the cancer incidences of general population were increased when they were exposed to PAHs (Nielsen et al, 1985;Baek et al, 1991 (IARC, 2010).…”

Section: Introductionmentioning

confidence: 94%

Characterization of Endocrine Disruptors and Particulate Matter Emissions from Laser Cutting of Plastic Film in the TFT-LCD Industry

Chan

Yuan

Tsai

et al. 2017

Aerosol Air Qual. Res.

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In the last five years laser cutting has been identified as one of the solutions for converting and shaping the plastic films used in the Thin Film Transistor Liquid Crystal Display (TFT-LCD) industry due to its positive effects on cost management. However, there has been quite limited information available with regard to the hazards of the air pollutants released during the laser cutting process, and in particular in relation to the real operations that occur in a factory. This is the first study that aims to assess the Endocrine Disruptors (EDs) and Particulate Matter (PM) emitted during the laser cutting process. Phthalate Esters (PAEs) and Polycyclic Aromatic Hydrocarbons (PAHs) are the representative EDs in this research. The results indicate that the power of the laser and type of plastic significantly affect the composition of air pollutants. Di(2-ethylhexyl) phthalate (DEHP) of PAEs was identified in the Laser Generated Air Contaminants (LGACs) from both Polycarbonate (PC) and Polyethylene Terephthalate (PET) films. DEHP was detected in the gas-phase LGACs of PC and PET at low, medium and high power (80 W, 160 W, 240 W), but not detected in the particle-phase LGACs of PC and PET, except when the laser was operated at a low power setting. With the laser power increased, the concentrations of PM and PAHs also increased. This research found that during laser cutting PC released more DEHP than PET, but PET released more PAHs than PC.

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Estrogenic activity of environmental polycyclic aromatic hydrocarbons in uterus of immature Wistar rats

Cited by 83 publications

References 73 publications

Effects of Exposure Period and Dose of Diethylstilbestrol on Pregnancy in Rats

Effects of Exposure Period and Dose of Diethylstilbestrol on Pregnancy in Rats

Ovariectomized mouse uterotrophic assay of 36 chemicals

Characterization of Endocrine Disruptors and Particulate Matter Emissions from Laser Cutting of Plastic Film in the TFT-LCD Industry

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