Estrogenic activity of cadmium, copper and zinc in the yeast estrogen screen

Denier, Xavier; Hill, Elisabeth M.; Rotchell, Jeanette M.; Minier, Christophe

doi:10.1016/j.tiv.2009.01.006

Cited by 45 publications

(19 citation statements)

References 20 publications

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“…Influence of DOM on the action of the natural estrogen 17β-estradiol (E2) was also studied. The estrogenic activity was tested by using the yeast estrogen screen (YES) assay, which has been widely used as a cost-effective and simple in vitro screening system to detect estrogenic activities of certain chemicals and environmental samples (Denier et al 2009;Ma et al 2007;Thomas et al 2009;Wang et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Estrogenic effects of dissolved organic matter and its impact on the activity of 17β-estradiol

Chen

Shen

Tang

et al. 2011

Environ Sci Pollut Res

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Section: Introductionmentioning

confidence: 99%

Estrogenic effects of dissolved organic matter and its impact on the activity of 17β-estradiol

Chen

Shen

Tang

et al. 2011

Environ Sci Pollut Res

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“…However, the organic extracted test solutions revealed significant (p \ 0.01) antiestrogenic effects at all e-waste recycling sites, except site E. The reason might be the combined pollution of both heavy metals and organic compounds. Accordingly to the previous results in this study, the influence was much higher at the sites with simple household e-waste recycling workshops around.The potentiation of E2 activity by metals has been reported by Denier et al (2009), also using the yeast estrogen screen assay. It was observed that cadmium, copper and zinc increaed the galactosidase activity of E2.…”

Section: Resultsmentioning

confidence: 76%

Occurrence of (Anti)estrogenic Effects in Surface Sediment from an E-Waste Disassembly Region in East China

Chen

Shen

et al. 2012

Bull Environ Contam Toxicol

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Because of the report on the abnormal local fertility rate at Taizhou area, which is a famous e-waste disassembly center in China, the hormone-like effects in the surface sediment from the local river was investigated. Compared to the control site DG, significant estrogenic effects (p < 0.01) were observed at e-waste recycling sites ranging from 6.01 to 29.31 nmol/kg dw E2 equivalents by water extraction while ranging from 20.47 to 135.02 nmol/kg dw by organic extraction. When coincubated with E2, the water and the organic extractions displayed significant (p < 0.01) synergistic and anti-estrogenic effects respectively.

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“…Stock solutions for all of the compounds were prepared at final chemical concentrations of 0.02 mol/L as reported by Denier et al (2009). Seven concentrations of chemical, ranging from 2 × 10 −9 mol/L to 2 × 10 −3 mol/L, were obtained by serial dilutions.…”

Section: Methodsmentioning

confidence: 99%

“…Heavy metals are widely dispersed in the environment and are a major concern in the modern industrial context of pollution because they tend to bioaccumulate (Denier et al 2009). Heavy metals have the ability to cause detrimental health effects by affecting reproduction, fertility, as well as the immune and neuronal systems, and by leading to carcinogenesis (Shiue 2015;Martins et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…In the last 15 years, the potential health threat posed by endocrine-disrupting chemicals (EDCs) has come to the forefront of toxicology and represents a major concern among the public community (Buha et al 2013). Several studies based on in vitro and in vivo assays have revealed that heavy metals, such as cadmium (Cd), mercury (Hg), and zinc (Zn), act as potential EDCs by activating estrogen receptor (ER) and modulating the estrogenic activity of endogenous hormones (Denier et al 2009;Isidori et al 2010;Ali et al 2010). However, the endocrine disruption induced by chemicals is not restricted to the sex hormone system but also includes thyroid hormone (TH) disruption (Murk et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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T-screen and yeast assay for the detection of the thyroid-disrupting activities of cadmium, mercury, and zinc

Liu

Ren

et al. 2016

Environ Sci Pollut Res

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In the present study, a two-hybrid yeast bioassay and a T-screen were used to screen for the thyroid receptor (TR)-disrupting activity of select metallic compounds (CdCl 2 , ZnCl 2 , HgCl 2 , CuSO 4 , MnSO 4 , and MgSO 4 ). The results reveal that none of the tested metallic compounds showed TRagonistic activity, whereas ZnCl 2 , HgCl 2 , and CdCl 2 demonstrated TR antagonism. For the yeast assay, the dose-response relationship of these metallic compounds was established, and the concentrations producing 20 % of the maximum effect of ZnCl 2 , HgCl 2 , and CdCl 2 were 9.1 × 10 −5 , 3.2 × 10 −6 , and 1.2 × 10 −6 mol/L, respectively. The T-screen also supported the finding that ZnCl 2 , HgCl 2 , and CdCl 2 decreased the cell proliferation at concentrations ranging from 10 −6 to 10 −4 mol/ L. Furthermore, the thyroid-disrupting activity of metallic compounds in environmental water samples collected from the Guanting Reservoir, Beijing, China was evaluated. Solid-phase extraction was used to separate the organic extracts, and a modified two-hybrid yeast bioassay revealed that the metallic compounds in the water samples could affect thyroid hormone-induced signaling by decreasing the binding of the thyroid hormone. The addition of ethylenediaminetetraacetic acid (30 mg/L) could eliminate the effects. Thus, the cause(s) of the thyroid toxicity in the water samples appeared to be partly related to the metallic compounds.

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Estrogenic activity of cadmium, copper and zinc in the yeast estrogen screen

Cited by 45 publications

References 20 publications

Estrogenic effects of dissolved organic matter and its impact on the activity of 17β-estradiol

Estrogenic effects of dissolved organic matter and its impact on the activity of 17β-estradiol

Occurrence of (Anti)estrogenic Effects in Surface Sediment from an E-Waste Disassembly Region in East China

T-screen and yeast assay for the detection of the thyroid-disrupting activities of cadmium, mercury, and zinc

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