Estrogen withdrawal in chick oviduct. Selective loss of high abundance classes of polyadenylated messenger RNA

Cox, Ronald F.

doi:10.1021/bi00634a022

Cited by 86 publications

(41 citation statements)

References 42 publications

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“…Individual eucaryotic mRNAs have characteristic rates of decay: some are very stable (hours), others decay rapidly (1 h or less), while some have variable rates of decay under different physiological conditions (1,3,6,7,13,14,16,19,20,22,24,30). It is not clear why classes of mRNAs with different stabilities exist, since little work has been performed to determine the factors responsible for mRNA stability.…”

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“…This implies that differ-ential mRNA stability may be one way of regulating not only the amount of a specific protein that is made but also the time during development that it is synthesized. In fact, this kind of regulation takes place during certain stages of development in the slime mold Dictyostelium discoideum (8,18,22), during induction of various proteins by prolactin (13) and estradiol (6,16), in histone synthesis specifically during the process of DNA replication (14), in the later stages of myogenesis (1) and erythropoeiesis (3,7), possibly in c-myc gene expression (7,20), and in the differential expression of photosynthesis genes in Rhodopseuidomonas capsulata (3). However, the specific mechanisms involved in eucaryotic mRNA decay remain to be elucidated.…”

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Role of protein synthesis in decay and accumulation of mRNA during spore germination in the cellular slime mold Dictyostelium discoideum.

Kelly¹,

Shaw²,

Ennis³

1987

Mol. Cell. Biol.

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Spore germination in Dictyostelium discoideum is a particularly suitable model for studying the regulation of gene expression, since developmentally regulated changes in both protein and mRNA synthesis occur during the transition from dormant spore to amoeba. The previous isolation of three cDNA clones specific for mRNA developmentally regulated during spore germination allowed for the quantitation of the specific itlRNAs during this process. The three mRNAs specific to clones pLK109, pLK229, and pRK270 have half-lives much shorter (minutes) than those of constitutive mRNAs (hours). Using spore germination as a model, we studied the roles of ribosome-mRNA interactions and protein synthesis in mRNA degradation by using antibiotics that inhibit specific reactions in protein biosynthesis. Cycloheximide inhibits the elongation step of protein synthesis. Polysomes accumulate in inhibited cells because ribosomes do not terminate normally and new ribosomes enter the polysome, eventually saturating the mRNA. Pactamycin inhibits initiation, and consequently polysomes break down in the presence of this drug. Under this condition, the mRNA is essentially free of ribosomes. pLK109, pLK229, and pRK270 mRNAs were stabilized in the presence of cycloheximide, but pactamycin had no effect on their normal decay. Since it seems likely that stability of mRNA reflects the availability of sites for inactivation by nucleases, it follows that in the presence of cycloheximide, these sites are protected, presumably by occupancy by ribosomes. No ribosomes are bound to mRNA in the presence of pactamycin, and therefore mRNA degrades at about the normal rate. The data further indicate that a labile protein is probably not involved in mRNA decay or stabilization, since protein synthesis is inhibited equally by both antibiotics. We conclude that it may be important to use more than one type of protein synthesis inhibitor to evaluate whether protein synthesis is required for mRNA decay. The effect of protein synthesis ihhibition on mRNA synthesis and accumulation was also studied. mRNA synthesis continues in the presence of the inhibitors, albeit at a diminished rate relative to that of the uninhibited control.

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Role of protein synthesis in decay and accumulation of mRNA during spore germination in the cellular slime mold Dictyostelium discoideum.

Kelly¹,

Shaw²,

Ennis³

1987

Mol. Cell. Biol.

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“…Specific changes in mRNA stability are known to occur in several differentiating systems (7,27,33) and during temperaturesensitive cell cycle arrest in yeasts (13 (18,31), whereas the latter does not (29). Thus, it may be that the decline in tk mRNA levels which accompanies terminal differentiation reflects the inability of postmitotic muscle cells to splice cellular tk transcripts.…”

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tk Enzyme expression in differentiating muscle cells is regulated through an internal segment of the cellular tk gene.

1984

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Thymidine kinase (tk) enzyme expression is shut down when cultured skeletal muscle cells terminally differentiate. This regulation is mediated by a rapid and specific decline in the abundance of cellular tk mRNA. tk-deficient mouse myoblasts were transformed to the tk-positive phenotype by using both the cellular tk gene of the chicken and the herpesvirus tk gene. Myoblasts transformed with the cellular tk gene effectively regulate tk enzyme activity upon terminal differentiation. Conversely, myoblasts transformed with the herpesvirus tk gene continue to express tk enzyme activity in postreplicative muscle cells. A regulated pattern of expression is retained when the promoter of the cellular tk gene is replaced by the promoter of the herpesvirus tk gene. Moreover, the cellular tk gene is appropriately regulated during terminal muscle differentiation when its 3' terminus is removed and replaced by the terminus of the viral tk gene. Thus, the element of the cellular tk gene sufficient to specify its regulation is entirely intragenic.

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“…This structure also binds hnRNP (Herman et al, 1978 ;Miller et al, 1978a ;Van Eekelen and Van Venrooij, 1981) and snRNP (Miller et a/., 1978b ;Gallinaro et al, 1983) Rousseau, 1985). RNA is a potent competitor for the binding of receptor-androgen (Liao et al, 1980), -oestrogen (Feldman et al, 1981 ;Chong and Lippman, 1982), and -dexamethasone (Tymoczko et al, 1982) (Palmiter and Carey, 1974 ;Cox, 1977) ; similarly, oestrogen or progesterone was demonstrated to affect the half-life of coinalbumin mRNA in chick oviduct (Mc Knight and Palmiter, 1979), and androgen to modulate the half-life of prostatic binding protein mRNAs (Page and Parker, 1982).…”

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confidence: 99%

Control of gene expression by steroid hormones

Béchet¹

1986

Reprod. Nutr. Dévelop.

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Estrogen withdrawal in chick oviduct. Selective loss of high abundance classes of polyadenylated messenger RNA

Cited by 86 publications

References 42 publications

Role of protein synthesis in decay and accumulation of mRNA during spore germination in the cellular slime mold Dictyostelium discoideum.

Role of protein synthesis in decay and accumulation of mRNA during spore germination in the cellular slime mold Dictyostelium discoideum.

tk Enzyme expression in differentiating muscle cells is regulated through an internal segment of the cellular tk gene.

Control of gene expression by steroid hormones

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