Estrogen stimulated lipogenic activity in the ovary of the freshwater prawn,Macrobrachium Rosenbergü

Abstract

Three consecutive-day injections with different doses (0.1, 0.25, 0.5, 1 .O, 2.0 and 4.0 pglg) of estradiol-170 (&) produced statistically significant increases in the cytosolic NADP-linked malate dehydrogenase and glucose-6-phosphate dehydrogenase activities in the ovary of the freshwater prawn (Macrobrachium rosenbergii) on the 4th day of treatment over the control values, depending upon the doses administered. Dosedependent increases in the malate dehydrogenase and glucose-6-phosphate dehydrogenase activities were observed between 0.1-0.25,0.25-0.5, and 0.5-1.0 pglg doses. A similar magnitude of response was found at 1 .O, 2.0 and 4.0 pglg dose in both the cases of malate dehydrogenase and glucose-6-phosphate dehydrogenase activities. A lower dose of 0.5 pglg was ineffective in eliciting any response to either enzyme. Ergosterol (2.0 pglg) did not evoke any change in the malate dehydrogenase and glucose-6-phosphate dehydrogenase activities in comparison to the control values.Simultaneous injection of tamoxifen (0.5 pglg) with E2 (2.0 pglg) caused an inhibition of the E2-induced rise in the glucose-6-phosphate dehydrogenase activity but failed to show similar changes in the case of malate dehydrogenase activity. Use of cycloheximide (0.5 mgll of immersion medium) also inhibited & (2.0 pg/g)-stimulated increase in the activities of these two enzymes. The data clearly show a subcellular action of estrogen with an indication of its role in lipogenesis in the ovary of the freshwater prawn.

Effect of vertebrate steroid hormones on the ovarian maturation stages of orange mud crab, Scylla olivacea (Herbst, 1796)

“…This be the case of 17α-hydroxyprogesterone which affects crab ovaries (Reddy et al, 2006). Comparatively, oestrogen introductions to the shrimp M. rosenbergii, triggers lipogenic activity in its ovaries (Ghosh and Ray, 1994). Likewise, when steroid-based hormones are introduced to the sword prawn, Parapenaeopsis hardwicki where such hormones accelerate its ovaries to develop for reproduction (Kulkarni et al, 1979).…”

Hemolymph levels of vertebrate-type steroid hormones in female kuruma prawn Marsupenaeus japonicus (Crustacea: Decapoda: Penaeidae) during natural reproductive cycle and induced ovarian development by eyestalk ablation

“…Progesterone and corticosterone affect the excitability of photoreceptors in the crayfish Procambarus digueti 23 . Estradiol‐17β has a subcellular action of ion transport and metabolic activation in the giant freshwater prawn Macrobrachium rosenbergii 24,25 . The vertebrate‐type steroid hormones in M. japonicus could have similar functions.…”

Effect of Estradiol-17ß on Embryonic Tolerance, Growth, and Muscular compactness of Giant Freshwater Prawn, Macrobrachium rosenbergii

“…In tadpoles, E2 stimulated aromatase activity to cause vitellogenesis in developing oocytes in the gonads [14]. It also stimulated lipogenic activity in the ovary and play a role in stimulation of female sexual maturation and egg production of the giant freshwater prawn [15].…”