Estrogen signaling in the medial amygdala decreases emotional stress responses and obesity in ovariectomized rats

Estrada, Christina M.; Ghisays, Valentina; Nguyen, Elizabeth T.; Caldwell, Jody L.; Streicher, Joshua; Solomon, M.B.

doi:10.1016/j.yhbeh.2017.12.002

Cited by 26 publications

(25 citation statements)

References 112 publications

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“…However, chemogenetic activation of SIM1 neurons changes neither food intake nor body temperature (Xu et al, 2015 ). These findings are somewhat inconsistent with prior findings (Xu et al, 2017 ; Estrada et al, 2018 ). Both local administration of estrogen to the MeA and subcutaneous estradiol treatment abolishes hyperphagia and body weight gain in ovariectomized female rodents (Xu et al, 2017 ; Estrada et al, 2018 ), suggesting that ER-α-expressing neurons in the MeA may have the ability to control feeding at least in female animals.…”

Section: Discussioncontrasting

confidence: 97%

“…The MeA contains estrogen receptor-α (ER-α)-expressing cells (Xu et al, 2015 ; Saito et al, 2016 ). Importantly, it has been well described that estrogen receptor-α (ER-α) activity in the MeA controls energy balance as well (Liu et al, 2013 ; Estrada et al, 2018 ). For example, local implantation of estradiol in the MeA or treatment with estradiol reduces food intake, body weight, and adiposity observed in ovariectomized female rodents (Xu et al, 2017 ; Estrada et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

“…Importantly, it has been well described that estrogen receptor-α (ER-α) activity in the MeA controls energy balance as well (Liu et al, 2013 ; Estrada et al, 2018 ). For example, local implantation of estradiol in the MeA or treatment with estradiol reduces food intake, body weight, and adiposity observed in ovariectomized female rodents (Xu et al, 2017 ; Estrada et al, 2018 ). Specific deletion of ER-α in SIM1 neurons in the MeA causes obesity in both male and female mice, while overexpression of ER-α prevents diet-induced obesity (DIO) in male mice (Xu et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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Activation of the ARCPOMC→MeA Projection Reduces Food Intake

Kwon

2020

Front. Neural Circuits

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Proopiomelanocortin (POMC) neurons in the arcuate nucleus of the hypothalamus (ARC) plays an essential role in the control of food intake and energy expenditure. Melanocortin-4 receptors (MC4Rs) are expressed in key areas that are implicated in regulating energy homeostasis. Although the importance of MC4Rs in the paraventricular hypothalamus (PVH) has been well documented, the role of MC4Rs in the medial amygdala (MeA) on feeding remains controversial. In this study, we specifically examine the role of a novel ARC POMC →MeA neural circuit in the regulation of short-term food intake. To map a local melanocortinergic neural circuit, we use monosynaptic anterograde as well as retrograde viral tracers and perform double immunohistochemistry to determine the identity of the neurons receiving synaptic input from POMC neurons in the ARC. To investigate the role of the ARC POMC →MeA projection on feeding, we optogenetically stimulate channelrhodopsin-2 (ChR2)-expressing POMC fibers in the MeA. Anterograde viral tracing studies reveal that ARC POMC neurons send axonal projections to estrogen receptor-α (ER-α)-and MC4R-expressing neurons in the MeA. Retrograde viral tracing experiments show that the neurons projecting to the MeA is located mainly in the lateral part of the ARC. Optogenetic stimulation of the ARC POMC →MeA pathway reduces short-term food intake. This anorectic effect is blocked by treatment with the MC4R antagonist SHU9119. In addition to the melanocortinergic local circuits within the hypothalamus, this extrahypothalamic ARC POMC →MeA neural circuit would play a role in regulating short-term food intake.

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Section: Discussioncontrasting

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Activation of the ARCPOMC→MeA Projection Reduces Food Intake

Kwon

2020

Front. Neural Circuits

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“…KCNQ3 KD mice were less anxious, spending more time in the center. Though not as useful as home cage observations, total distance traveled and average velocity (Fig 5E-F) provide insight into energy expenditure [63,64]. Once again, KCNQ3 mice were different than control animals with significantly lower distance and velocity.…”

Section: Crispr Knockdown Of Kcnq3 Enhances Susceptibility Of Males Tmentioning

confidence: 93%

CRISPR Knockdown ofKcnq3Attenuates the M-current in NPY/AgRP Neurons

Stincic¹,

Bosch²,

Hunker

et al. 2020

Preprint

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Arcuate nucleus Neuropeptide Y/Agouti-related peptide (NPY/AgRP) neurons drive ingestive behavior in response to the internal and external environment of an organism. NPY/AgRP neurons are adjacent to the median eminence, a circumventricular organ, and circulating metabolic factors and hormones communicate the energy state of the animal via these neurons by altering the excitability of NPY/AgRP neurons, which produces an appropriate change in behavior to maintain homeostasis. One example of this plasticity is seen in the M-current, a subthreshold, non-inactivating K + current that acts to modulate excitability. Fasting decreases while estradiol increases the M-current through regulation of subunit mRNA expression of Kcnq 2, 3, & 5. KCNQ2/3 heteromers are thought to mediate the majority of the M-current. Here we used a recently developed single adeno-associated viral (AAV) vector containing a recombinasedependent Staphylococcus aureus Cas9 (SaCas9) and a single guide RNA against Kcnq3 to selectively delete Kcnq3 in NPY/AgRP neurons to produce a loss of function in the M-current. We found that this virus was effective at knocking down Kcnq3 but not Kcnq2 expression. With the reduced KCNQ3 channel expression NPY/AgRP neurons were more depolarized, exhibited a higher input resistance, and the rheobase current needed to induce firing was significantly reduced, indicative of increased excitability. Although the resulting decrease in the M-current did not overtly alter ingestive behavior, it did significantly reduce the locomotor activity as measured in open field testing. Therefore, the SaCas9-sgKcnq3 is efficient to knock down Kcnq3 expression thereby reducing the M-current and increasing the excitability of NPY/AgRP neurons.

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“…They take part in the process of differentiating between the parts of the brain responsible for social and sexual behaviour [12,13] (in the study by Mogi et al [13], expression of oestrogen receptor α [ERα] at mRNA levels in the hypothalamus and hippocampus was higher in male mice than in female mice before birth, while mRNA expression of oestrogen receptor β [ERβ] in the prefrontal cortex was higher in female mice immediately after birth). Oestrogens are also attributed a significant share in the regulation of motivational processes (including reproductive behaviour), social behaviour, and aggression [14], as well as memory processes and attention [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Expression of ESR1 and ESR2 oestrogen receptor encoding gene and personality traits – preliminary study

Talarowska

Szemraj

2019

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Introduction: The main objective of the study is to examine the hypothesis claiming a correlation between personality traits measured with the use of the Minnesota Multiphasic Personality Inventory (MMPI-2) personality questionnaire and the expression of the ERα (ESR1) and ERβ (ESR2) encoding gene in patients suffering from depression. Material and methods: The experiment was carried out on a group of 44 individuals with depression. The Polish variant of the MMPI-2 was applied with the aim of assessing personality traits. Furthermore, the authors evaluated the expression of the genes encoding the oestrogen receptors (ERα and ERβ) at the mRNA level and protein level in the studied population. Results: No significant differences in the expression of ERα and ERβ encoding genes were found and confirmed in the patients with the first episode of depression and those suffering from subsequent episodes of the disease. No differences were found between women and men, either. In women a positive relationship was found between the scale of psychopathy (p = 0.04), paranoia (p = 0.01), and mania (p = 0.03) and expression for the ERα encoding gene at the mRNA level. A negative relationship was found between the mania scale and ERβ encoding gene expression at mRNA (p = 0.03) and protein (p = 0.04) levels. In males a positive relationship between anxiety as a personality trait and expression of the ERβ receptor encoding gene at mRNA level (p = 0.03) and protein level (p = 0.03) was found. Conclusions: Personality traits may be linked with the expression of genes encoding oestrogen receptors (ERα and ERβ) among patients with depressive disorders.

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Estrogen signaling in the medial amygdala decreases emotional stress responses and obesity in ovariectomized rats

Cited by 26 publications

References 112 publications

Activation of the ARCPOMC→MeA Projection Reduces Food Intake

Activation of the ARCPOMC→MeA Projection Reduces Food Intake

CRISPR Knockdown ofKcnq3Attenuates the M-current in NPY/AgRP Neurons

Expression of ESR1 and ESR2 oestrogen receptor encoding gene and personality traits – preliminary study

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