Estrogen sensitive liver transgenic zebrafish (Danio rerio) line (Tg(vtg1:mCherry)) suitable for the direct detection of estrogenicity in environmental samples

Abstract

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Identification of receptors for eight endocrine disrupting chemicals and their underlying mechanisms using zebrafish as a model organism

“…50% of its LC 50 value (261 μg/L). In contrast, exposure levels for the four steroid hormones (E1, E2, E3 and EE2), as positive controls for the estrogenic effect, were all chosen to be 10 μg/L according to their 96-hpf EC 50 values of 2.9-8.9 μg/L (Segner et al, 2003;Van den Belt et al, 2004;Bakos et al, 2019). The control group was treated with 0.0025% acetone (Zhang et al, 2018); no obvious phenotypic malformations were observed for larval and adult zebrafish at this acetone concentration.…”

Larval zebrafish as an in vitro model for evaluating toxicological effects of mycotoxins

“…Due to the strong relation of VTG protein for detecting estrogenicity effects at low levels in some strains, Bakos et al (2019) developed a transgenic zebrafish line (Tg(vtg1:mCherry)) natural vitellogenin-1 promoter sequence. Embryos 5 dpf of this zebrafish line were tested through a protocol based on the VTG and an estrogenic effect was detectable after seven generations and ZEA 100 ng/L, revealing the suitability of this model for detecting such effect and also the strong estrogenic capacity of ZEA (Schwartz et al, 2010;Bakos et al, 2013 and.…”

Use of All‐Male cyp17a1‐Deficient Zebrafish (Danio rerio) for Evaluation of Environmental Estrogens

“…Previously, Chen et al (2010) generated a transgenic line of ere ‐ zvtg1 : green fluorescent protein (GFP) zebrafish, which was reported to be responsive to 0.1 μg/L E2 and 1000 μg/L BPA administered for 13 days, as evidenced by green fluorescence accumulation. In another study, a transgenic line ( vtg1 :mCherry Tg fish) was reported to detect E2 at 0.1 μg/L after 2 days and 0.005 μg/L after 7 days and to measure the estrogenicity in wastewater samples, as reflected by the red fluorescence in the liver (Bakos et al, 2019).…”