Estrogen Selectively Up-Regulates the Phospholipid Hydroperoxide Glutathione Peroxidase in the Oviducts

Lapointe, Jérôme; Kimmins, Sarah; MacLaren, Leslie A.; Bilodeau, Jean‐François

doi:10.1210/en.2004-1373

Cited by 50 publications

(39 citation statements)

References 80 publications

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“…GPX4 is well known for its function in suppressing cell death and reduction in hydrogen peroxide (Imai et al 1996, Agbor et al 2014. Increase in E 2 induces the GPX4 production in bovine oviduct (Lapointe et al 2005). This evidence indicates that E 2 and the oxidative stress preventing enzymes act in concert to protect the embryos against ROS exposure.…”

Section: Figurementioning

confidence: 74%

Oviduct: roles in fertilization and early embryo development

Winuthayanon

2017

Journal of Endocrinology

206

191

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Animal oviducts and human Fallopian tubes are a part of the female reproductive tract that hosts fertilization and pre-implantation development of the embryo. With an increasing understanding of roles of the oviduct at the cellular and molecular levels, current research signifies the importance of the oviduct on naturally conceived fertilization and pre-implantation embryo development. This review highlights the physiological conditions within the oviduct during fertilization, environmental regulation, oviductal fluid composition and its role in protecting embryos and supplying nutrients. Finally, the review compares different aspects of naturally occurring fertilization and assisted reproductive technology (ART)-achieved fertilization and embryo development, giving insight into potential areas for improvement in this technology.

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Section: Figurementioning

confidence: 74%

Oviduct: roles in fertilization and early embryo development

Winuthayanon

2017

Journal of Endocrinology

206

191

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“…Oviduct cell culture situation in vivo . GPX4 transcripts in the bovine oviduct were found to be almost restricted to the luminal epithelium, with highest levels in the isthmus proximal to the dominant follicle during the follicular stage, and in the post-ovulatory period (Lapointe et al 2005).…”

Section: Discussionmentioning

confidence: 96%

A bovine oviduct epithelial cell suspension culture system suitable for studying embryo–maternal interactions: morphological and functional characterization

Rottmayer¹,

Ulbrich²,

Kölle³

et al. 2006

Reproduction

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We established a short-term (24 h) culture system for bovine oviduct epithelial cells (BOECs), obtained on day 3.5 of the estrous cycle and evaluated the cells with respect to morphological criteria, marker gene expression, and hormone responsiveness. BOEC sheets were isolated mechanically from the ampulla with similar yields from oviducts ipsi-and contralateral to the ovulation site (57.9G4.6 and 56.4G8.0!10 6 cells). BOECs showed O95% purity and cells cultured for 24 h maintained morphological characteristics present in vivo, as determined by light microscopy, scanning electron microscopy, and transmission electron microscopy. Both secretory cells with numerous secretory granules and ciliated cells with long, well-developed, and vigorously beating kinocilia were visible. Quantitative real-time PCR failed to detect significant differences in transcript levels between ipsiand contralateral BOECs for the majority of marker genes (estrogen receptors a and b (ESR1 and ESR2), 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR), oviductal glycoprotein 1 (OVGP1), progesterone receptor (PGR), and tumor rejection antigen 1 (TRA1)) throughout the 24 h culture period. However, the combined data of all time points for glutathione peroxidase 4 (GPX4), a gene previously shown to be expressed at higher levels in the ipsilateral oviduct in vivo, also indicated significantly different mRNA levels in vitro. The expression of marker genes remained stable after 6 h cell culture, indicating only a short adaptation period. Western blot analysis confirmed ESR1 and PGR protein expression throughout the culture period. In agreement with cyclic differences in vivo, estradiol-17b stimulation increased PGR transcript abundance in BOECs. Our novel culture system provides functional BOECs in sufficient quantities for holistic transcriptome and proteome studies, e.g. for deciphering early embryo-maternal communication.

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“…Beneficial effects of estrogens in several other tissues, such as lens epithelial cells, arteries, central nervous system, fat, liver, and oviducts, are also shown to result from improved defense against oxidative stress (42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53). Importantly, in view of the fact that E 2 inhibits oxidized LDL (54) inhibition of 15-lipoxygenase, the enzyme responsible for the generation of oxidized low density lipoprotein, partially prevents OVX-induced bone loss (55).…”

Section: Discussionmentioning

confidence: 99%

Skeletal Involution by Age-associated Oxidative Stress and Its Acceleration by Loss of Sex Steroids

Almeida

Han

Martin-Millan

et al. 2007

Journal of Biological Chemistry

609

617

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Both aging and loss of sex steroids have adverse effects on skeletal homeostasis, but whether and how they may influence each others negative impact on bone remains unknown. We report herein that both female and male C57BL/6 mice progressively lost strength (as determined by load-to-failure measurements) and bone mineral density in the spine and femur between the ages of 4 and 31 months. These changes were temporally associated with decreased rate of remodeling as evidenced by decreased osteoblast and osteoclast numbers and decreased bone formation rate; as well as increased osteoblast and osteocyte apoptosis, increased reactive oxygen species levels, and decreased glutathione reductase activity and a corresponding increase in the phosphorylation of p53 and p66 shc , two key components of a signaling cascade that are activated by reactive oxygen species and influences apoptosis and lifespan. Exactly the same changes in oxidative stress were acutely reproduced by gonadectomy in 5-month-old females or males and reversed by estrogens or androgens in vivo as well as in vitro. We conclude that the oxidative stress that underlies physiologic organismal aging in mice may be a pivotal pathogenetic mechanism of the age-related bone loss and strength. Loss of estrogens or androgens accelerates the effects of aging on bone by decreasing defense against oxidative stress.Age-related loss of bone mass and strength is an invariable feature of human biology, affecting women and men alike. Moreover, population-based studies demonstrate that substantial bone loss begins as early as the 20s in young adult women and men, long before any hormonal changes (1).3 The extent to which estrogen deficiency contributes to age-related bone loss and the slower rate of decline of bone mass and strength during the late postmenopausal years, and the molecular and cellular mechanisms of such putative interactions, are unknown.The universality of age-associated bone loss irrespective of sex steroid status notwithstanding, age is by far a more critical determinant of fracture risk than bone mass in humans indicating that age-related increase in fracture risk reflects a loss of bone strength that is only partly accounted for by loss of bone mass (2). Whereas an increased propensity to fall due to agerelated decline in neuromuscular function is a factor, there are also age-related changes in the bone itself. Such changes include disrupted architecture, altered composition of the bone mineral and matrix, delayed repair of fatigue microdamage, excessive turnover, and inadequate bone size (3-7). The most recently appreciated qualitative factor is loss of osteocytes (8, 9), former osteoblasts entombed into the mineralized matrix. Osteocyte death may influence the signals necessary for mechanical adaptation and repair and also lead to long term changes in bone hydration. The anti-apoptotic effect of sex steroids on osteocytes, which has been well documented in mice, rats, and humans (10 -12), may contribute to their anti-fracture efficacy independently of...

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Estrogen Selectively Up-Regulates the Phospholipid Hydroperoxide Glutathione Peroxidase in the Oviducts

Cited by 50 publications

References 80 publications

Oviduct: roles in fertilization and early embryo development

Oviduct: roles in fertilization and early embryo development

A bovine oviduct epithelial cell suspension culture system suitable for studying embryo–maternal interactions: morphological and functional characterization

Skeletal Involution by Age-associated Oxidative Stress and Its Acceleration by Loss of Sex Steroids

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