Estrogen‐regulated expression of a growth arrest specific gene (gas‐1) in rat uterus

Abstract

gas-1 belongs to a family of growth arrest specific genes negatively regulated after growth induction of arrested cells. We report the expression of gas-1 in an in vivo system of cell proliferation. gas-1 mRNA accumulates progressively in the uterus of ovariectomized rats with a peak at three weeks after surgery. After estrogen treatment gas-1 mRNA levels decrease within two hours, at a time when c-myc expression is greatly increased, and return to pretreatment levels at 48 hours. Treatment with cycloheximide does not prevent estrogen-induced down-regulation of gas-1 mRNA levels. The present results show that: i) estrogen affects the uterine growth state by regulating the expression of both positively and negatively acting genes, ii) gas-1 expression is controlled by cellular growth state also in vivo.

Identification and Expression of a New Sulfhydryl Oxidase SOx-3 during the Cell Cycle and the Estrus Cycle in Uterine Cells

“…There is little information concerning the negative estrogen action on gene regulation. However, it has been demonstrated that, in the rat uterus, E 2 represses the expression of gas-1 which belongs to the growth arrest-specific gene family (12). This study clearly established that estrogens can affect the uterine growth state by down-regulating the expression of negatively acting genes.…”

Effects of Gas1 on gliomas: a review on current preclinical studies

“…Moreover, transgenic mice that express Src under the transcriptional control of the glial fibrillary acidic protein develop hypervascularized glioblastomas with morphological and molecular characteristics of human GBM. [64,65] On [66] Little is known about the transcription factors that upregulate gas1. For example the transcription factor Tbox5 increases the activity of the mouse gas1 promoter.…”

Characterization of a uterine luminal fluid protein ULF‐250 using N‐terminal microsequencing and RT‐PCR identifies a novel estrogen‐regulated gene in the rat uterus

“…Other proteins expressed in the uterus and secreted into the lumen under hormonal influence include the presumptive induced protein [8], alpha uterine protein (AUP) [9,10], the estrogen responsive induced protein (IP) Ill], which was later shown to be a creatine kinase [12], a cat uterine protein that is estrogen dependent (CUPED), which was localized in the endometrial epithelial cells [13], and a mouse lactotransferrin [14]. In addition, estradiol regulates the expression of growth arrest specific (gas) genes [15,16], growth factors and their receptors [17,18]. Estradiol administered to immature rats stimulated the synthesis and secretion of proteins by the uterus [19-211. These proteins include complement C3 and immunoglobulin [22,23].…”