Estrogen receptors α and β in the inner ear of the ‘Turner mouse’ and an estrogen receptor β knockout mouse

Stenberg, Annika Elmqvist; Wang, H; Sahlin, Lena; Stierna, Pontus; Enmark, Eva; Hultcrantz, Malou

doi:10.1016/s0378-5955(02)00310-6

Cited by 33 publications

(18 citation statements)

References 27 publications

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“…These mice have no estrogen production, but a normal pattern of ERs (Stenberg et al 2002), and their hearing loss seems to be of cochlear origin with a central component engaging the eighth cranial nerve, resembling that of the ER-b K/K mice. Since 'Turner mice' are estrogen deficient, it is not surprising that defects in these mice resemble those found in ER-b K/K mice.…”

Section: Discussionmentioning

confidence: 93%

“…In young (3 months) ER-b knock out mice (BERKO or ER-b K/K ), the inner ear appeared to be normal, except for swollen afferent nerve endings (Stenberg et al 2002), and when exposed to acoustic trauma these mice were more vulnerable to hearing loss than were wild-type (WT) littermates (Meltser et al 2008). It is also known that in the brain of the ER-b K/K mice, with increasing age, neuronal loss was observed (Wang et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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Inner ear pathology and loss of hearing in estrogen receptor-β deficient mice

Simonoska¹,

Stenberg²,

Duan

et al. 2009

Journal of Endocrinology

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There are well known differences between males and females in hearing. In the present study, the role of estrogen receptor-b (ER-b; listed as ESR2 in the MGI Database) in hearing was investigated by comparing hearing and morphology of the inner ear in ER-b knock-out mice (ER-b) with that of wild-type (WT) littermates. Hearing was analyzed with auditory brainstem response audiometry at 3 and 12 months. The ER-b K/K mice were deaf at 1 year of age, and the morphological analysis showed absence of hair cells and loss of the whole organ of Corti initiated in the basal turn of the cochlea. Furthermore, in ER-b, but not in WT mice, the spiral ganglion was lacking many of its neurons.Immunostaining showed the presence of both ER-a (listed as ESR1 in the MGI Database) and ER-b in the nuclei of some neurons in the inner ear in WT mice, but no ER-b was found in the ER-b K/K mice as expected. ER-a staining was predominant in the nuclei of large neurons and ER-b in nuclei of small neurons and fibroblasts. These results reveal that both ERs are present in the inner ear at specific localizations suggesting subtype-specific functions. It is concluded that ER-b is important for the prevention of agerelated hearing loss. These findings strengthen the hypothesis that estrogen has a direct effect on hearing functions.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Inner ear pathology and loss of hearing in estrogen receptor-β deficient mice

Simonoska¹,

Stenberg²,

Duan

et al. 2009

Journal of Endocrinology

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“…However, differential expression of this network is a novel observation in IE development. Additionally, while estrogen receptor expression has been previously observed in the adult mouse IE (Stenberg et al 1999;Stenberg et al 2002), our observation that multiple components of this signaling pathway are differentially expressed in the developing IE is novel. It is interesting to note that in a separate study of gene expression in avian IE sensory epithelial regeneration we also observed changes in estrogen receptor signaling, including estrogen receptor a.…”

Section: Discussionmentioning

confidence: 63%

“…Components of estrogen receptor signaling have been previously described in the developing and adult IE (Stenberg et al 1999;Stenberg et al 2002; also reviewed in Hultcrantz et al 2006), but this analysis provides a much higher level of detail for this pathway. Figure 4, A, B, and D, shows components of the IGF-1, FGF, and Neurotrophin-Trk signaling pathways, respectively.…”

Section: Microdissection Of Ie and Adjacent Nie Tissuesmentioning

confidence: 99%

Toward a Systems Biology of Mouse Inner Ear Organogenesis: Gene Expression Pathways, Patterns and Network Analysis

2007

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We describe the most comprehensive study to date on gene expression during mouse inner ear (IE) organogenesis. Samples were microdissected from mouse embryos at E9-E15 in half-day intervals, a period that spans all of IE organogenesis. These included separate dissections of all discernible IE substructures such as the cochlea, utricle, and saccule. All samples were analyzed on high density expression microarrays under strict statistical filters. Extensive confirmatory tests were performed, including RNA in situ hybridizations. More than 5000 genes significantly varied in expression according to developmental stage, tissue, or both and defined 28 distinct expression patterns. For example, upregulation of 315 genes provided a clearcut ''signature'' of early events in IE specification. Additional, clear-cut, gene expression signatures marked specific structures such as the cochlea, utricle, or saccule throughout late IE development. Pathway analysis identified 53 signaling cascades enriched within the 28 patterns. Many novel pathways, not previously implicated in IE development, including b-adrenergic, amyloid, estrogen receptor, circadian rhythm, and immune system pathways, were identified. Finally, we identified positional candidate genes in 54 uncloned nonsyndromic human deafness intervals. This detailed analysis provides many new insights into the spatial and temporal genetic specification of this complex organ system.

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“…Nevertheless, human studies investigating the effect of sex hormones on hearing show an influence of estrogen (Horner, 2009). The estrogen receptors, alpha and beta, are localized where electric impulses are transmitted (inner and outer hair cells, spiral ganglion) and where inner-ear homeostasis is maintained (stria vascularis, spiral ligament) (Stenberg et al, 2002); therefore, estrogens seem to have a role in signal transmission and cochlear homeostasis.…”

mentioning

confidence: 99%

Aging of the auditory system

Roth

2015

The Human Auditory System - Fundamental Organization and Clinical Disorders

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Estrogen receptors α and β in the inner ear of the ‘Turner mouse’ and an estrogen receptor β knockout mouse

Cited by 33 publications

References 27 publications

Inner ear pathology and loss of hearing in estrogen receptor-β deficient mice

Inner ear pathology and loss of hearing in estrogen receptor-β deficient mice

Toward a Systems Biology of Mouse Inner Ear Organogenesis: Gene Expression Pathways, Patterns and Network Analysis

Aging of the auditory system

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