Estrogen receptor β is the predominant isoform expressed in the brain of adult and fetal sheep

Deitch, Helen R.; Mershon, John L.; Clark, Kenneth E.

doi:10.1067/mob.2001.115223

Cited by 4 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mismatches have been reported between the developmental profiles of mRNA and protein abundance for steroid receptors in the sheep brain , suggesting that additional regulation occurs at the translational and post‐translational levels. In addition to ERα and AR, a second form of the oestrogen receptor ERβ is expressed in the sheep brain but was not measured in the present study. Although not shown to play a role in SDN development, ERβ has been implicated in the oestrogen‐dependent organisation of gonadotrophin signalling pathways in rats .…”

Section: Discussionmentioning

confidence: 61%

Sex Differences in Expression of Oestrogen Receptor α but not Androgen Receptor mRNAs in the Foetal Lamb Brain

Reddy

Estill

Meaker

et al. 2014

J Neuroendocrinology

View full text Add to dashboard Cite

Gonadal steroid hormones play important roles during critical periods of development to organize brain structures that control sexually dimorphic neuroendocrine responses and behaviors. Specific receptors for androgens and estrogens must be expressed at appropriate times during development in order to mediate these processes. The present study was performed to test for sex differences in the relative expression of estrogen receptor-α (ERα) and androgen receptor (AR) mRNA during the window of time in gestation that is critical for behavioral masculinization and differentiation of the ovine sexually dimorphic nucleus (oSDN) in the sheep. In addition, we examined whether ERα and AR mRNA expression is localized within the nascent oSDN and could be involved in its development. Using quantitative RT-PCR, we found that females expressed more ERα mRNA than males in medial preoptic area and medial basal hypothalamus during the mid-gestational critical period for brain sexual differentiation. No sex differences were found for AR mRNA in any tissue examined or for ERα in amygdala and frontal cortex. Using radioactive in situ hybridization, we found that the distributions of ERα and AR mRNA overlapped with aromatase mRNA, which delineates the boundaries of the developing oSDN and identifies this nucleus as a target for both androgens and estrogens These data demonstrate that the transcriptional machinery for synthesizing gonadal steroid receptors is functional in the fetal lamb brain during the critical period for sexual differentiation and suggest possible mechanisms for establishing dimorphisms controlled by gonadal steroids may exist at the level of steroid hormone receptor expression.

show abstract

Section: Discussionmentioning

confidence: 61%

Sex Differences in Expression of Oestrogen Receptor α but not Androgen Receptor mRNAs in the Foetal Lamb Brain

Reddy

Estill

Meaker

et al. 2014

J Neuroendocrinology

View full text Add to dashboard Cite

show abstract

“…The methods and primers used in the present study were identical to those of Deitch et al (9). Briefly, total RNA was extracted with Trizol reagent (GIBCO-BRL) and underwent reverse transcription into cDNA (Superscript; GIBCO-BRL) with oligodeoxythymidine primer.…”

Section: Rna Extraction and Rt-pcr Reactionmentioning

confidence: 99%

“…Both ER␣ and ER␤ are expressed in the rat, primate, and human vasculature (25,32,35). Recently, we have shown that ovine brain contains both ER␣ and ER␤ (9). Estrogen receptors are nuclear receptors that transduce extracellular signals, resulting in transcriptional responses (35), and the levels of the two forms are tissue and species specific (14,15,25,32,35).…”

mentioning

confidence: 99%

Hemodynamic effects of acute and repeated exposure to raloxifene in ovariectomized sheep

Zoma

Baker²,

Mershon³

et al. 2006

American Journal of Physiology-Heart and Circulatory Physiology

Self Cite

View full text Add to dashboard Cite

We hypothesize that administration of acute and daily doses of raloxifene will have significant effects on ovine coronary and uterine hemodynamics and that these changes are estrogen receptor dependent. Eleven ovariectomized sheep were instrumented to measure mean arterial pressure, heart rate (HR), cardiac output (CO), and coronary (CBF) and uterine artery blood flows (UBF). A dose-response curve was generated for raloxifene (1, 3, and 10 microg/kg) and compared with a standard dose of estradiol-17beta (1 microg/kg) given intravenously. In a second group of animals, raloxifene (10 microg.kg-1.day-1) was administered intravenously for 14 consecutive days, and cardiovascular responses were compared with a group of animals administered estradiol-17beta (10 microg/kg) daily for the same period. To determine whether raloxifene-related vascular responses were estrogen receptor (ER) mediated, the animals were pretreated with estrogen antagonist ICI-182,780 given intravenously. Finally, RT-PCR was preformed to determine the presence of ERalpha and ERbeta mRNA in ovine coronary and uterine vessels. Raloxifene increased CBF and UBF dose dependently with a parallel decrease in the associated vascular resistances. Acute cardiovascular responses to daily doses of raloxifene and estradiol-17beta were sustainable. In contrast to estradiol-17beta, which significantly increases CO by increasing HR but not stroke volume, raloxifene significantly increased stroke volume without a significant parallel increase in HR. ICI-182,780 abolished raloxifene-induced hemodynamic responses, and ERalpha and ERbeta mRNA are present in both ovine coronary and uterine vessels. Hence, the hemodynamic effects of raloxifene are dose dependent, sustainable, and estrogen receptor mediated.

show abstract

Development of ER-α and ER-β expression in the developing ovine brain and pituitary

Schaub

Gersting

Keller‐Wood

et al. 2008

Gene Expression Patterns

View full text Add to dashboard Cite

Estrogen receptor β is the predominant isoform expressed in the brain of adult and fetal sheep

Cited by 4 publications

References 7 publications

Sex Differences in Expression of Oestrogen Receptor α but not Androgen Receptor mRNAs in the Foetal Lamb Brain

Sex Differences in Expression of Oestrogen Receptor α but not Androgen Receptor mRNAs in the Foetal Lamb Brain

Hemodynamic effects of acute and repeated exposure to raloxifene in ovariectomized sheep

Development of ER-α and ER-β expression in the developing ovine brain and pituitary

Contact Info

Product

Resources

About