Estrogen receptor α is involved in the estrogenic regulation of arginine vasopressin immunoreactivity in the supraoptic and paraventricular nuclei of ovariectomized rats

Grassi, Daniela; Amorim, Miguel A.R.; Garcia-Segura, Luis Miguel; Panzica, Giancarlo

doi:10.1016/j.neulet.2010.03.022

Cited by 27 publications

(32 citation statements)

References 27 publications

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“…However, neurons expressing ERα are projecting to both the PVN [45,46] and SON [47]. Therefore, the final response of the nitrergic system through ERs would probably be dependent on the balance of a local direct action (GPER and ERβ) and an indirect action through the afferent neurons regulated by ERα, similar to what has been hypothesized for the vasopressin system [48]. …”

Section: Discussionmentioning

confidence: 87%

NADPH-Diaphorase Colocalizes with GPER and Is Modulated by the GPER Agonist G1 in the Supraoptic and Paraventricular Nuclei of Ovariectomized Female Rats

Grassi

Lagunas²,

Pinos³

et al. 2016

Neuroendocrinology

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Nitric oxide is produced in the brain by the neuronal nitric oxide synthase (nNOS) and carries out a wide range of functions by acting as a neurotransmitter-like molecule. Gonadal hormones are involved in the regulation of the brain nitrergic system. We have previously demonstrated that estradiol, via classical estrogen receptors (ERs), regulates NOS activity in the supraoptic (SON) and paraventricular (PVN) nuclei of the hypothalamus, acting through both ERα and ERβ. Magnocellular and parvocellular neurons in the SON and PVN also express the G protein-coupled ER (GPER). In this study, we have assessed whether GPER is also involved in the regulation of nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase in the SON and PVN. Adult female ovariectomized rats were treated with G1, a selective GPER agonist, or with G1 in combination with G15, a selective GPER antagonist. G1 treatment decreased NADPH-diaphorase expression in the SON and in all PVN subnuclei. The treatment with G1 + G15 effectively rescued the G1-dependent decrease in NADPH-diaphorase expression in both brain regions. In addition, the activation of extracellular signal-regulated kinase (ERK) 1/2, one of the kinases involved in the GPER-dependent intracellular signaling pathway and in NOS phosphorylation, was assessed in the same brain nuclei. Treatment with G1 significantly decreased the number of p-ERK 1/2-positive cells in the SON and PVN, while the treatment with G1 + G15 significantly recovered its number to control values. These findings suggest that the activation of GPER in the SON and PVN inhibits the phosphorylation of ERK 1/2, which induces a decrease in NADPH-diaphorase expression.

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Section: Discussionmentioning

confidence: 87%

NADPH-Diaphorase Colocalizes with GPER and Is Modulated by the GPER Agonist G1 in the Supraoptic and Paraventricular Nuclei of Ovariectomized Female Rats

Grassi

Lagunas²,

Pinos³

et al. 2016

Neuroendocrinology

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“…The presence of gonadal steroids certainly does not eliminate the control of AVP release, but it could modulate the response. Additionally, we cannot rule out a possible indirect regulation of estrogen on OT and AVP neurones via ER-a, which is expressed in neurones located in the organum vasculosum of lamina terminalis and the medial preoptic area, where projections to the SON originate (Voisin et al 1997, Sladek & Somponpun 2007, Grassi et al 2010. However, these brain areas were not a focus of this work.…”

Section: Discussionmentioning

confidence: 97%

Effect of the interaction between food state and the action of estrogen on oxytocinergic system activity

Lucio-Oliveira¹,

Franci²

2011

Journal of Endocrinology

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Increased plasma osmolality by food intake evokes augmentation of plasma oxytocin (OT). Ovarian steroids may also influence the balance of body fluids by acting on OT neurones. Our aim was to determine if estrogen influences the activity of OT neurones in paraventricular nucleus (PVN) and supraoptic nucleus (SON) under different osmotic situations. Ovariectomized rats (OVX) were treated with either estradiol (E 2 ) or vehicle and were divided into three groups: group I was fed ad libitum, group II underwent 48 h of fasting, and group III was refed after 48 h of fasting. On the day of the experiment, blood samples were collected to determine the plasma osmolality and OT. The animals were subsequently perfused, and OT/FOS immunofluorescence analysis was conducted on neurones in the PVN and the SON. When compared to animals which were fasted or fed ad libitum, the plasma osmolality of refed animals was higher, regardless of whether they were treated with vehicle or E 2 . We observed neural activation of OT cells in vehicle-or E 2 -treated OVX rats refed after 48 h of fasting, but not in animals fed ad libitum or in animals that only underwent 48 h of fasting. Finally, the percentage of neurones that co-expressed OT and FOS was lower in both the PVN and the SON of animals treated with E 2 and refed, when compared to vehicle-treated animals. These results suggest that E 2 may have an inhibitory effect on OT neurones and may modulate the secretion of OT in response to the increase of osmolality induced by refeeding.

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“…Estrogen may exert an inhibitory effect on AVP secretion in response to osmotic stimuli, which could be mediated through this receptor (Sladek and Somponpun, 2008;Grassi et al, 2010). Therefore, we also assessed the ERa-IR of the OVLT, dMnPO, and vMnPO.…”

Section: Discussionmentioning

confidence: 99%

“…Indirect action of estrogen on OT and AVP neurons in rats via estrogen receptor subtype a (ERa) cannot be ruled out (Somponpun et al, 2004;Grassi et al, 2010) because the neurons of the MnPO and OVLT that express these receptors project to the SON (Voisin et al, 1997). In castrated rats, estrogen treatment either significantly reduces (Suzuki and Handa, 2004) or induces no change in (Greco et al, 2001) ERb expression in the PVN.…”

Section: Introductionmentioning

confidence: 99%

Modulation of the activity of vasopressinergic neurons by estrogen in rats refed with normal or sodium-free food after fasting

2015

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Estrogen receptor α is involved in the estrogenic regulation of arginine vasopressin immunoreactivity in the supraoptic and paraventricular nuclei of ovariectomized rats

Cited by 27 publications

References 27 publications

NADPH-Diaphorase Colocalizes with GPER and Is Modulated by the GPER Agonist G1 in the Supraoptic and Paraventricular Nuclei of Ovariectomized Female Rats

NADPH-Diaphorase Colocalizes with GPER and Is Modulated by the GPER Agonist G1 in the Supraoptic and Paraventricular Nuclei of Ovariectomized Female Rats

Effect of the interaction between food state and the action of estrogen on oxytocinergic system activity

Modulation of the activity of vasopressinergic neurons by estrogen in rats refed with normal or sodium-free food after fasting

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