Estrogen: Physiology, Pharmacology, and Formulations for Replacement Therapy

Ruggiero, Ronald J.; Likis, Frances E

doi:10.1016/s1526-9523(02)00233-7

Cited by 85 publications

(59 citation statements)

References 16 publications

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“…Regarding hormone replacement therapy, in recent years, concerns have been raised by studies such as the Women's Health Initiative, regarding the risks associated with the use of synthetically produced hormones [4]. Combining the autologous nature of iPSCs with the potential to differentiate iPSC-derived EBs into steroidogenic cells, it will be possible to produce bioidentical hormones for patient treatment [38]. A potential future direction with the presented microfluidic platform will be to further evaluate human iPSCs for clinically relevant applications.…”

Section: Toward Personalized Medicinementioning

confidence: 99%

Functional Maintenance of Differentiated Embryoid Bodies in Microfluidic Systems: A Platform for Personalized Medicine

Güven

Lindsey

Poudel

et al. 2015

Stem Cells Translational Medicine

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Hormone replacement therapies have become important for treating diseases such as premature ovarian failure or menopausal complications. The clinical use of bioidentical hormones might significantly reduce some of the potential risks reportedly associated with the use of synthetic hormones. In the present study, we demonstrate the utility and advantage of a microfluidic chip culture system to enhance the development of personalized, on-demand, treatment modules using embryoid bodies (EBs). Functional EBs cultured on microfluidic chips represent a platform for personalized, patient-specific treatment cassettes that can be cryopreserved until required for treatment. We assessed the viability, differentiation, and functionality of EBs cultured and cryopreserved in this system. During extended microfluidic culture, estradiol, progesterone, testosterone, and anti-müllerian hormone levels were measured, and the expression of differentiated steroidogenic cells was confirmed by immunocytochemistry assay for the ovarian tissue markers anti-müllerian hormone receptor type II, follicle-stimulating hormone receptor, and inhibin b-A and the estrogen biosynthesis enzyme aromatase. Our studies showed that under microfluidic conditions, differentiated steroidogenic EBs continued to secrete estradiol and progesterone at physiologically relevant concentrations (30-120 pg/ml and 150-450 pg/ml, respectively) for up to 21 days. Collectively, we have demonstrated for the first time the feasibility of using a microfluidic chip system with continuous flow for the differentiation and extended culture of functional steroidogenic stem cell-derived EBs, the differentiation of EBs into cells expressing ovarian antigens in a microfluidic system, and the ability to cryopreserve this system with restoration of growth and functionality on thawing. These results present a platform for the development of a new therapeutic system for personalized medicine. STEM CELLS TRANSLATIONAL MEDICINE 2015;4:261-268

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Section: Toward Personalized Medicinementioning

confidence: 99%

Functional Maintenance of Differentiated Embryoid Bodies in Microfluidic Systems: A Platform for Personalized Medicine

Güven

Lindsey

Poudel

et al. 2015

Stem Cells Translational Medicine

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“…Abbott IMX [19] Corning ACS:180 [8] DPC Coat-a-Count [16] In-House Extraction [4] DPC Double Ab [22] Boehringer ES700 [5] DPC E2-6 [8] Boehringer ES300 [8] IDS Gamma-B [4] Wallac DELFIA [22] J&J Amerlite [9] DPC Immulite [9] Sorin [4] Serono Maia [4] INCStar Clin Assays [7] remaining in-house extraction RIA users have often performed better than market-leading immunoassay platforms in terms of comparability (see Figure 2) and recovery. Only three laboratories in the UK maintain these assays.…”

Section: Oestradiol Bias and Variability Of The Bias (Var) Versus Id-mentioning

confidence: 99%

“…These include effects on the bone, skin and muscle, liver, brain, vascular function and lipid metabolism. Good reviews of the various functions of oestradiol have been written by Ruggerio et al 4 and Gruber et al…”

Section: Introductionmentioning

confidence: 99%

Oestradiol assays: fitness for purpose?

Middle

Kane

2009

Ann Clin Biochem

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In this review we discuss the analytical inadequacies of oestradiol assays in relation to the clinical requirements for performing them, and make recommendations for their improvement. The measurement of oestradiol can be requested in a number of clinical scenarios ( precocious puberty, infertility, assisted conception, hormone replacement therapy). The very wide dynamic range of oestradiol concentrations is a huge challenge for routine assays, which they are unlikely to meet on theoretical as well as practical grounds. The EQA performance of oestradiol assays in terms of trueness, comparability, recovery and analytical sensitivity leaves much to be desired and indicates that calibration is compromised by poor analytical specificity. To make oestradiol assays fit for purpose requires concerted action by all stakeholders to define analytical quality specifications for the various clinical scenarios involved, and then to encourage concerted action by the diagnostic industry to use the steroid reference measurement system to improve specificity, trueness and traceability.

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“…Estrogens influence the growth and function of female and male reproductive tissues, maintain the skeletal and central nervous system, protect the cardiovascular system and prevent colon cancer and skin aging [19,51]. Considering the numerous effects estrogens have on the human body, it is not surprising to consider the potential of phytoestrogens for human health.…”

Section: + -Independent Inducible Nos (Inos)mentioning

confidence: 99%

Extract of Rubus coreanus Fruits Increases Expression and Activity of Endothelial Nitric Oxide Synthase in the Human Umbilical Vein Endothelial Cells

Yoon¹,

Park²,

Oh³

et al. 2011

Journal of Life Science

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This study aimed to investigate the effects of water extract of Rubus coreanus (RCE) on the expression and activity of endothelial nitric oxide synthase (eNOS), as well as its signal transduction pathways in human umbilical vein endothelial cells (HUVECs). The specific inhibitors of NOS show RCE treatment increases NO production in HUVECs due to the up-regulation of eNOS rather than iNOS. The real-time expression level of eNOS mRNA was also increased upon RCE treatment in HUVECs. While a PKC-specific inhibitor, RO-317549, did not alter RCE-induced NO production in HUVECs, tamoxifen (estrogen receptor-specific inhibitor), PD98059 (ERK-specific inhibitor) and LY-294002 (PI3K/Akt-specific inhibitor) did have suppressive effects. Increased NO production by RCE seems to result from a higher level of active eNOS (pSer1177). Specifically, inhibition of ERK not only decreased the level of active eNOS, but also increased the inactive form of the enzyme (pThr495) in HUVECs. This study suggests that RCE treatment increases NO production in HUVECs due to the increased expression and activity of eNOS. It is also shown that RCE-induced eNOS activation occurs partly through the binding of RCE to the estrogen receptor, along with ERK and PI3K/Akt-dependent signal transduction pathways. In addition, the regulatory binding proteins of eNOS including Hsp90 and caveolin-1 were related to these effects of RCE on eNOS activity in HUVECs.

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Estrogen: Physiology, Pharmacology, and Formulations for Replacement Therapy

Cited by 85 publications

References 16 publications

Functional Maintenance of Differentiated Embryoid Bodies in Microfluidic Systems: A Platform for Personalized Medicine

Functional Maintenance of Differentiated Embryoid Bodies in Microfluidic Systems: A Platform for Personalized Medicine

Oestradiol assays: fitness for purpose?

Extract of Rubus coreanus Fruits Increases Expression and Activity of Endothelial Nitric Oxide Synthase in the Human Umbilical Vein Endothelial Cells

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