Estrogen Modulates Cytokine Expression in Human Periodontal Ligament Cells

Liu, Shu; Guan, Su-Min; Fu, Shan-Min; Guo, Tao; Cao, Meng; Ding, Yin

doi:10.1177/154405910808700214

Cited by 71 publications

(49 citation statements)

References 28 publications

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“…The consequence of ovariectomy was an increase in sRANKL levels in all the studied groups. Our results are consistent with data of Shevde et al [17] who have shown suppression of RANKL-induced osteoclast differentiation by estrogens via c-Jun repression and results yielded by Shu et al [18] who documented reversion of stimulatory effect of lipopolysaccharide on IL-6 and RANKL expression after estrogen administration. Ovariectomy in mice has been shown to increase IL-6 and RANKL expression in bone tissue [19].…”

Section: Discussionsupporting

confidence: 93%

Role of interleukin-6 on RANKL-RANK/osteoprotegerin system in hypothyroid ovariectomized mice.

Myśliwiec

Zbucki²,

Nikołajuk³

et al. 2011

Folia Histochem Cytobiol.

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Postmenopausal women frequently develop hypothyroidism. Estrogen depletion is accompanied by an increase of IL-6, accelerating bone turnover. The influence of hypothyroidism on bone metabolism in postmenopausal women is poorly understood. The aim of the study was an attempt to clarify the role of interleukin-6 on RANKL-RANK/osteoprotegerin system in hypothyroid ovariectomized mice. The study was performed on 56, 12-13 weeks old, female mice: C57BL/6J (wild-type; WT) and C57BL/6J IL6-/-Kopf (IL-6 knock-out; IL6KO). The mice were randomly divided into 8 groups with 7 mice in each one: 1/ WT controls, 2/ IL6KO controls, 3/ WT hypothyroid mice, 4/ IL6KO hypothyroid mice, 5/ WT ovariectomized, 6/ IL6KO ovariectomized, 7/ WT ovariectomized hypothyroid mice and 8/ IL6KO ovariectomized hypothyroid mice. Experimental model of menopause was produced by bilateral ovariectomy carried out in 8-9 weeks old mice. Experimental model of hypothyroidism was induced by propylthiouracyl administration in driking water. The serum levels of TRACP 5b, osteocalcin, OPG and RANKL were determined by ELISA.Serum RANKL concentrations were elevated significantly in all groups of ovariectomized mice as compared to respective controls, but in a minor degree in IL6KO hypothyroid mice as compared to wild-type animals. Moreover sRANKL values were significantly lower in IL6KO as compared to WT controls and IL6KO PTU injected mice. Osteoprotegerin serum levels were decreased in all IL-6 deficient mice and in a highest degree in sham-operated hypothyroid mice. To sum up, the results of the present study suggest that estrogens deficit is a strong stimulus for RANKL-RANK/OPG pathway that breaks an inhibitory influence of hypothyroidism even in IL-6 deficient mice.

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Section: Discussionsupporting

confidence: 93%

Role of interleukin-6 on RANKL-RANK/osteoprotegerin system in hypothyroid ovariectomized mice.

Myśliwiec

Zbucki²,

Nikołajuk³

et al. 2011

Folia Histochem Cytobiol.

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“…21,22 According to our results, we can speculate that hormonal changes during pregnancy have an impact on local biochemical parameters in saliva. Table 2.…”

supporting

confidence: 52%

“…The study design included the following groups: 1) pregnant women (n=96) who were in their second trimester (weeks [16][17][18][19][20][21][22][23][24] or third trimester (weeks 25-34), 2) postpartum women (n=96) who were evaluated 6 months after delivery.…”

Section: Study Populationmentioning

confidence: 99%

Evaluation Of Salivary IL-1Beta And IL-6 Levels In Pregnant And Postpartum Women

Gümüş¹,

Öztürk²,

Belibasakis³

et al. 2016

EÜ Dişhek Fak Derg

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ÖZETAmaç: Gebelikte ortaya çıkan fizyolojik değişiklikler lokal enflamatuvar medyatörleri etkileyebilir ve periodontal enflamasyonun şiddetini arttırabilir. Çalışmamızda aynı kadında gebelikte ve doğum sonrasında tükürük IL-1beta (interlökin-1beta) ve IL-6 (interlökin-6) düzeylerinin incelenmesi ve periodontal hastalık şiddeti ile ilişkisinin değerlendirilmesi amaçlanmıştır. Yöntem: Toplam 96 gingivitis tanısı konan kadın çalışmaya dahil edilmiştir. Gebe kadınlar doğum sonrası 6. ayda tekrar değerlendirilmiş, gebe grubu ve doğum sonrası olmak üzere iki grup oluşturulmuştur. Tükürük IL-1beta ve IL-6 düzeyleri ELISA yöntemi ile belirlenmiştir. Parametreler arasında ki korelasyonlar Spearman's korelasyon analizi ile değerlendirilmiştir. Bulgular: Tükürük IL-1beta düzeyleri doğum sonrası grubunda gebe gruba göre anlamlı olarak düşük bulunmuştur (p=0.045). Doğum sonrası grupta IL-6 seviyeleri gebe grubundan anlamlı olarak yüksek düzeydedir (p<0.0001). Gebe grubunda IL-1beta seviyesi plak indeksi, kanama indeksi ve sondalama derinliği ile anlamlı pozitif korelasyon göstermektedir. Doğum sonrası grupta IL-6 düzeyleri plak ve kanama skorları ile pozitif korelasyondadır. Sonuç: Çalışmamızın bulgularına göre, lokal biyokimyasal parametreler gebelikten etkilenmektedir. Bu ilişkilerin anlaşılması diş hekimleri ve tıbbi uzmanlar açısından önem taşımaktadır. Anahtar Kelimeler: Tükürük, periodontal hastalık, gebelik ABSTRACT Introduction: Local inflammatory mediators could be affected from physiological changes in the pregnancy and exacerbate the periodontal inflammation. The aim of the present study was to evaluate pregnant and postpartum saliva levels of and

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“…In vitro, they found a dose of estrogen stimulation enhanced osteogenic differentiation ability by detecting the expression of ER and osteogenic genes (Syed and Khosla 2005;Wang et al 2006). Similarly, estrogen may modulate cytokine expression and regulate the character of human PDLCs (Cao et al 2007;Shu et al 2008). These findings made us wonder whether PDLSCs have the same character when exposed to specific microenvironment such as estrogen-deficient environment.…”

Section: Discussionmentioning

confidence: 99%

Estrogen Deficiency Leads to Impaired Osteogenic Differentiation of Periodontal Ligament Stem Cells in Rats

Zhang

Liu

Zhou

et al. 2011

Tohoku J. Exp. Med.

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Estrogen deficiency in post-menopausal women is considered as one of the risk factors for periodontal diseases. The periodontal ligament is a connective tissue that connects cementum and alveolar bone to constrain teeth within the jaw. Periodontal ligament stem cells (PDLSCs) isolated from the periodontal ligament can differentiate into many types of specialized cells, including osteoblast-like cells that can be used to regenerate alveolar bone. However, little is known about the effect of estrogen-deficient microenvironment on the osteogenic differentiation of PDLSCs. The aim of this study was to explore the role of estrogen on the potential for osteogenic differentiation of PDLSCs using a rat model of osteoporosis. Three-month-old female Sprague-Dawley rats were divided into two groups (n = 6 for each): ovariectomized (OVX) and sham-operated rats (Sham). Then the characteristics of PDLSCs isolated from these rats were investigated. Real-time PCR analysis showed the lower expression levels of estrogen receptors (ERα and ERβ) mRNAs in PDLSCs of OVX animals compared to Sham control. Mineralization assay demonstrated fewer calcium deposits in PDLSCs from OVX group than those from Sham group. Treatment with 17β-estradiol (E2) significantly enhanced the osteogenic differentiation of PDLSCs from both groups in vitro. Furthermore, by means of lentivirus-mediated siRNA targeting ERα or ERβ, the expression of ERα or ERβ was down-regulated (> 50% reduction), which impaired the estrogen-induced osteogenic differentiation of PDLSCs from both groups (> 50% reduction). These results indicate that estrogen plays an important role in maintaining osteogenic differentiation of PDLSCs, which acts through both ERα and ERβ.

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Estrogen Modulates Cytokine Expression in Human Periodontal Ligament Cells

Cited by 71 publications

References 28 publications

Role of interleukin-6 on RANKL-RANK/osteoprotegerin system in hypothyroid ovariectomized mice.

Role of interleukin-6 on RANKL-RANK/osteoprotegerin system in hypothyroid ovariectomized mice.

Evaluation Of Salivary IL-1Beta And IL-6 Levels In Pregnant And Postpartum Women

Estrogen Deficiency Leads to Impaired Osteogenic Differentiation of Periodontal Ligament Stem Cells in Rats

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