Estrogen Inhibits Development of Yolk Veins and Causes Blood Clotting in Transgenic Medaka Fish Overexpressing Estrogen Receptor

Kawamura, Toshijiro; Sakai, Shuichi; Omura, Seiichi; Hori-e, Ryota; Kawahara, Tetsuya; Kinoshita, Masato; Yamashita, Ichiro

doi:10.2108/zsj.19.1355

Cited by 13 publications

(7 citation statements)

References 41 publications

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“…Numerous studies have been performed in a variety of fish species to assess the effects of a potent environmental estrogen originating from birth control pills, 17␣-ethynylestradiol (EE 2 ), on vitellogenin production [1][2][3][4], endocrine and reproductive function [5,6], and cells of reproductive organs and the kidneys and liver [7]. Some studies have examined the antagonistic role of the antiestrogen used in the treatment and prevention of breast cancer, tamoxifen (TMX), in protecting fish against the disrupting action of EE 2 [8][9][10]. The actions of environmental estrogens in wastewater on aquatic populations appear to confirm the effects seen in controlled laboratory settings [11], including the induction of ovotestes [12].…”

Section: Introductionmentioning

confidence: 99%

Tamoxifen Protects Against 17α-Ethynylestradiol-Induced Liver Damage and the Development of Urogenital Papillae in the Rainbow Darter (Etheostoma caeruleum)

Elias¹,

Kalombo²,

Mercurio³

2007

Environ Toxicol Chem

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Juvenile rainbow darters (Etheostoma caeruleum) were exposed to nominal concentrations of 20 to 1,000 ng/L of 17␣ethynylestradiol (EE 2 ) at 120 d posthatch and in a subsequent experiment to 200 ng/L of EE 2 with 2.0 to 20,000 ng/L of tamoxifen (TMX) at 150 d posthatch to determine the threshold of estrogen-induced morphological and histological changes in a sexually dimorphic benthic fish species ecologically relevant to southern Minnesota (USA). 17␣-Ethynylestradiol induced female-associated urogenital papillae in males at 200 ng/L, enlargement and development of fibrosis in male testes, enlargement of ovary and oocyte size in females, and large fatty inclusions in the liver of both sexes. Exposure to 1,000 ng/L of EE 2 caused gross hypertrophy of the liver and kidneys and high mortalities, predominantly in male fish. A low incidence of ovotestes found in all treatment groups was unaffected by EE 2 , which may be unusual to this species or a response to unknown water contaminants present during the hatching or early development of the darters. Gonadosomatic index was not altered for either sex by any treatment. A TMX level equal to or less than that of EE 2 decreased fat accumulation in the liver in both sexes, and a TMX level greater than that of EE 2 appeared to prevent urogenital papilla in males. Tamoxifen did not significantly alter fibrosis caused by EE 2 in testes. It appears that the presence of TMX in the environment can mask many signs of estrogen exposure, including secondary sexual characteristics, hypertrophy of ovaries and testes, and fatty infiltration of organs. Ovotestes did not prove to be a good indicator of estrogen exposure at this late stage of juvenile darter development. Keywords-Rainbow darterEstrogen Tamoxifen Liver GonadsEnviron. Toxicol. Chem. 26, 2007 E.E. Elias et al.

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Section: Introductionmentioning

confidence: 99%

Tamoxifen Protects Against 17α-Ethynylestradiol-Induced Liver Damage and the Development of Urogenital Papillae in the Rainbow Darter (Etheostoma caeruleum)

Elias¹,

Kalombo²,

Mercurio³

2007

Environ Toxicol Chem

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“…Transgenic fish are useful for detecting the effects of chemicals during in vivo experiments. Kawamura et al [25] reported that transgenic embryos of medaka could be used to assay the blood-clotting activity of estrogenic compounds in vivo. Amanuma et al [26] detected environmental mutagens using transgenic zebrafish.…”

Section: Introductionmentioning

confidence: 99%

Quantitative bio‐imaging analysis for evaluation of sexual differentiation in germ cells of olvas‐GFP/ST‐II YI medaka (Oryzias latipes) nanoinjected in ovo with ethinylestradiol

Hano

Oshima

et al. 2005

Enviro Toxic and Chemistry

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We examined the effects on sexual differentiation of nanoinjecting ethinylestradiol (EE2) into embryos of olvas-GFP/ST-II YI medaka (Oryzias latipes). This strain contains the green fluorescent protein (GFP) gene fused to the regulatory region of the medaka vasa gene, and germ-cell-specific expression of GFP can be visualized in living (transparent) individuals. The number of germ cells in untreated genotypic females (XX) was approximately 10-fold that in untreated genotypic males (XY) at 10 d posthatch (dph). Germ cell proliferation was prevented in XX females that developed from embryos nanoinjected with 0.5, 2.5, or 5.0 ng of EE2. Some 10-dph XY males from embryos injected with 0.5 ng or more of EE2 showed a larger fluorescent area and more germ cells than those of pooled control groups. Males and females from embryos injected with 5.0 ng of EE2 had no significant difference in germ cell number or fluorescent area. Thus, EE2 injection into embryos caused abnormal gonadal development in both sexes. Observations of external secondary sex characteristics and histological examination of adult gonads showed complete sex reversal in some males after 0.5-, 2.5-, and 5.0-ng treatments but no changes in XX females after any treatment. Thus, quantitative bio-imaging can aid in evaluating the effects of endocrine-disrupting chemicals on fish within 10 dph.

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“…Numerous studies have been performed in a variety of fish species to assess the effects of a potent environmental estrogen originating from birth control pills, 17α‐ethynylestradiol (EE 2 ), on vitellogenin production [1–4], endocrine and reproductive function [5,6], and cells of reproductive organs and the kidneys and liver [7]. Some studies have examined the antagonistic role of the antiestrogen used in the treatment and prevention of breast cancer, tamoxifen (TMX), in protecting fish against the disrupting action of EE 2 [8–10]. The actions of environmental estrogens in wastewater on aquatic populations appear to confirm the effects seen in controlled laboratory settings [11], including the induction of ovotestes [12].…”

Section: Introductionmentioning

confidence: 99%

Tamoxifen protects against 17α‐ethynylestradiol‐induced liver damage and the development of urogenital papillae in the rainbow darter (Etheostoma caeruleum)

Elias

Kalombo

Mercurio

2007

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Juvenile rainbow darters (Etheostoma caeruleum) were exposed to nominal concentrations of 20 to 1,000 ng/L of 17alpha-ethynylestradiol (EE2) at 120 d posthatch and in a subsequent experiment to 200 ng/L of EE2 with 2.0 to 20,000 ng/L of tamoxifen (TMX) at 150 d posthatch to determine the threshold of estrogen-induced morphological and histological changes in a sexually dimorphic benthic fish species ecologically relevant to southern Minnesota (USA). 17Alpha-ethynylestradiol induced female-associated urogenital papillae in males at 200 ng/L, enlargement and development of fibrosis in male testes, enlargement of ovary and oocyte size in females, and large fatty inclusions in the liver of both sexes. Exposure to 1,000 ng/L of EE2 caused gross hypertrophy of the liver and kidneys and high mortalities, predominantly in male fish. A low incidence of ovotestes found in all treatment groups was unaffected by EE2, which may be unusual to this species or a response to unknown water contaminants present during the hatching or early development of the darters. Gonadosomatic index was not altered for either sex by any treatment. A TMX level equal to or less than that of EE2 decreased fat accumulation in the liver in both sexes, and a TMX level greater than that of EE2 appeared to prevent urogenital papilla in males. Tamoxifen did not significantly alter fibrosis caused by EE2 in testes. It appears that the presence of TMX in the environment can mask many signs of estrogen exposure, including secondary sexual characteristics, hypertrophy of ovaries and testes, and fatty infiltration of organs. Ovotestes did not prove to be a good indicator of estrogen exposure at this late stage of juvenile darter development.

show abstract

Estrogen Inhibits Development of Yolk Veins and Causes Blood Clotting in Transgenic Medaka Fish Overexpressing Estrogen Receptor

Cited by 13 publications

References 41 publications

Tamoxifen Protects Against 17α-Ethynylestradiol-Induced Liver Damage and the Development of Urogenital Papillae in the Rainbow Darter (Etheostoma caeruleum)

Tamoxifen Protects Against 17α-Ethynylestradiol-Induced Liver Damage and the Development of Urogenital Papillae in the Rainbow Darter (Etheostoma caeruleum)

Quantitative bio‐imaging analysis for evaluation of sexual differentiation in germ cells of olvas‐GFP/ST‐II YI medaka (Oryzias latipes) nanoinjected in ovo with ethinylestradiol

Tamoxifen protects against 17α‐ethynylestradiol‐induced liver damage and the development of urogenital papillae in the rainbow darter (Etheostoma caeruleum)

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