Estrogen influences satellite cell activation and proliferation following downhill running in rats

Enns, Deborah L.; Tiidus, Peter M.

doi:10.1152/japplphysiol.00128.2007

Cited by 131 publications

(145 citation statements)

References 50 publications

(78 reference statements)

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“…There were no differences (P Ͼ 0.05) in body weight between any of the groups at Pre, whereas, at Post, body weight was lower (P Ͻ 0.05) in E compared with both S and P. There were no differences (P Ͼ 0.05) in body weight between S and P at Post. The lower body weights observed for E relative to S are in accordance with previous studies using ovariectomized rats (6,26,42). The plasma concentrations of estradiol and progesterone of all treatment groups are also shown in Table 1.…”

Section: Resultssupporting

confidence: 89%

“…Animals were returned to their cages and allowed to recover for 8 days before beginning the exercise protocol. We have demonstrated previously that 1 wk of estrogen exposure in this model is sufficient to allow estrogen effects on skeletal muscle to manifest (6,43). This recovery time allowed the pellet-implanted groups to receive prolonged exposure to the ovarian sex hormone.…”

Section: Methodsmentioning

confidence: 83%

“…The downhill running protocol employed in this study has been shown to induce structural protein damage (17), oxidative damage as indicated by protein carbonyl formation in soleus muscle (51), disruption of the membrane systems involved in excitation-contraction coupling (39), neutrophil and macrophage infiltration (5,45), and muscle satellite cell proliferation (6). Comparisons between fiber types within the same muscle indicate that type II fibers sustain greater eccentric damage than type I fibers (40).…”

Section: Discussionmentioning

confidence: 94%

“…Animals were acclimatized for a period of 1 wk in the laboratory before being randomly assigned to one of three treatment groups (n ϭ 16 per group): sham (S), progesterone (P), and estrogen (E). Estrogen (0.25 mg 17␤-estradiol, 21-day time release pellet; Innovative Research of America, Sarasota, FL) and progesterone (25 mg progesterone, 21-day time release pellet, Innovative Research of America) supplementation were administered via subcutaneous pellet implantation (6,43). Briefly, animals were anesthetized via inhaled isoflurane, and a small incision was made in the skinfolds of the neck.…”

Section: Methodsmentioning

confidence: 99%

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Effects of ovarian sex hormones and downhill running on fiber-type-specific HSP70 expression in rat soleus

Bombardier¹,

Vigna²,

Iqbal³

et al. 2009

Journal of Applied Physiology

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Bombardier E, Vigna C, Iqbal S, Tiidus PM, Tupling AR. Effects of ovarian sex hormones and downhill running on fiber-type-specific HSP70 expression in rat soleus. J Appl Physiol 106: 2009 -2015, 2009. First published April 9, 2009 doi:10.1152/japplphysiol.91573.2008This study examined the influence of the ovarian sex hormones, estrogen and progesterone, on the fiber-type-specific response of the inducible 70-kDa heat shock protein (HSP70) to damaging exercise in rat soleus. Ovariectomized female rats were divided into three treatment groups (n ϭ 16 per group): sham (S), progesterone (P; 25 mg pellet), and estrogen (E; 0.25 mg pellet). Each treatment group was divided into control and exercised groups. After 8 days of sham or hormone treatment, animals ran downhill intermittently for 90 min (17 m/min, Ϫ13.5°grade) on a treadmill, and soleus muscles were removed 24 h postexercise. HSP70 expression was assessed in whole muscle homogenates by Western blotting and in individual muscle fiber types by immunohistochemical analysis of serial cross sections of soleus samples. Comparisons between control groups showed that HSP70 expression in soleus was increased (P Ͻ 0.05) in E compared with both S and P. No difference (P Ͼ 0.05) was observed between S and P. Following downhill running, HSP70 content in soleus was increased (P Ͻ 0.05) compared with control in S and P, but not (P Ͼ 0.05) in E. As a result, soleus HSP70 content following downhill running was not different (P Ͼ 0.05) between any of the treatment groups. Under all conditions, HSP70 content was higher in type I vs. type II fibers, and the effects of both estrogen and exercise on HSP70 expression in soleus were also more pronounced in type I vs. type II fibers. These results demonstrate that 1) estrogen regulates HSP70 expression in skeletal muscle, increasing basal HSP70 expression and preventing further increases in HSP70 in response to exercise; 2) progesterone is not involved in the regulation of HSP70 expression in skeletal muscle; and 3) the effects of estrogen and exercise on HSP70 expression in skeletal muscle are fiber type specific. estrogen; progesterone; muscle fiber type HEAT SHOCK PROTEINS (HSP) are families of highly conserved stress proteins found in almost all cells, which have been shown to be induced by a variety of environmental and intracellular stresses (18). The inducible 70-kDa HSP (HSP70) may be the most highly induced protein of the cellular stress response and is rapidly upregulated under conditions of oxidative stress (48). It is well established that HSP70 content in skeletal muscle increases in response to both nondamaging and damaging exercise and can protect against contraction-induced muscle damage (for review, see Refs. 10,19). However, the HSP70 response to exercise in both skeletal (31, 33) and cardiac muscle (32) is sex specific, with males demonstrating a more robust response than females. Collectively, these studies and others have shown that the sex-specific HSP70 response to exercise is mediated by the ovarian sex hormo...

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Section: Resultssupporting

confidence: 89%

Section: Methodsmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Effects of ovarian sex hormones and downhill running on fiber-type-specific HSP70 expression in rat soleus

Bombardier¹,

Vigna²,

Iqbal³

et al. 2009

Journal of Applied Physiology

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“…Recent evidence suggests that oestrogen receptors (ERα and ERβ) within the muscle can stimulate the proliferation of satellite cells via the PI3K/Akt pathway for muscle growth and repair 33,40 . EE increases the proliferation of satellite cells in rat muscle tissue 41 , indicating that the synthetic component of hormonal contraceptives may have a direct anabolic e ect on muscle, possibly due to a local activation of IGF-1 pathway through an autocrine or paracrine manner 42 .…”

Section: Is There a Relationship Between Hormonal Contraceptives And mentioning

confidence: 99%

A perspective on current research investigating the effects of hormonal contraceptives on determinants of female athlete performance

Martín

Elliott‐Sale

2016

Rev. bras. educ. fís. esporte

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Sex, fiber-type, and age dependent in vitro proliferation of mouse muscle satellite cells

et al. 2011

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During postnatal growth and after muscle injury, satellite cells proliferate and differentiate into myotubes to form and repair musculature. Comparison of studies on satellite cell proliferation and differentiation characteristics is confounded by the heterogeneity of the experimental conditions used. To examine the influence of sex, age, and fiber-type origin on in vitro properties of satellite cells derived from postnatal muscles, fast extensor digitorum longus (EDL) and slow soleus (SOL) muscles were extracted from male and female mice of 1 week to 3 months of age. Myoblast proliferation and myogenic regulatory factor (MRF) expression was measured from cultures of freshly isolated satellite cells. Higher proliferation rate and elevated Myod1 expression was found in male EDL and SOL derived cells compared with females at age of 40, 60, and 120 days, whereas inverse tendency for cell proliferation was apparent in EDL of juvenile (7-day-old) pups. Myogenin and Mrf4 transcripts were generally elevated in males of 40 and 60 days of age and in female EDL of juveniles. However, these differentiation markers did not significantly correlate with proliferation rate at all ages. Pax7, whose overexpression can block myogenesis, was up-regulated especially in 40-day-old females where MRF expression was low. These results indicate that gender, postnatal age, and muscle fiber origin affect proliferation and muscle transcription factor expression in vitro. The results also support the view that satellite cells originating from slow and fast muscles are intrinsically different and warrant further studies on the effect of cell origin for therapeutic approaches.

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Estrogen influences satellite cell activation and proliferation following downhill running in rats

Cited by 131 publications

References 50 publications

Effects of ovarian sex hormones and downhill running on fiber-type-specific HSP70 expression in rat soleus

Effects of ovarian sex hormones and downhill running on fiber-type-specific HSP70 expression in rat soleus

A perspective on current research investigating the effects of hormonal contraceptives on determinants of female athlete performance

Sex, fiber-type, and age dependent in vitro proliferation of mouse muscle satellite cells

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