Estrogen enhances differentiation of osteoblasts in mouse bone marrow culture

Qu, Qiang; Perälä-Heape, M.; Kapanen, Anita; Dahllund, Johanna; Salo, Juha; Väänänen, H. Kalervo; Härkönen, Pirkko

doi:10.1016/s8756-3282(97)00276-7

Cited by 228 publications

(142 citation statements)

References 35 publications

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“…Thus the direct actions of E2 on marrow stromal cells appear to promote, or at least maintain, expression of osteoblastic phenotype in these mixed cell populations. These effects, while small in magnitude, are consistent with reported actions of E2 to promote osteoblast progenitor cell differentiation [12,26] or phenotypic expression of osteoblastic cells [ 19,291. These biochemical measurements were supported by colony formation assays.…”

Section: Discussionsupporting

confidence: 87%

“…The effects of E2 on cell proliferation in different cell strains reflect the range of direct in vitro E3 effects on osteoblastic cells or marrow stromal cells reported in the literature. Enhanced proliferation in response to E2 by osteoblasts or marrow stromal cells was demonstrated in mouse [19,26], rat [8,9,12,13] and human cell systems [S9]. Growth inhibition was reported by Shamay et al [30], who found that reduced cell growth was also associated with increased ALP expression in a niurine stromal cell line.…”

Section: Discussionmentioning

confidence: 98%

“…Again, a range of seemingly conflicting results have been reported. In contrast to the ex vivo studies demonstrating that estrogen withdrawal increased osteoblastogenesis [14,41], Qu et al [26] showed that direct E3 treatment of mouse bone marrow stromal cells in culture promoted cell proliferation as well as mineralized nodule formation. Estrogen also stimulated expression of ALP in a mouse marrow stromal cell line MBA-IS, although in this system proliferation was decreased [30].…”

Section: Introductionmentioning

confidence: 90%

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Estrogen regulation of growth and alkaline phosphatase expression by cultured human bone marrow stromal cells

Holzer

Einhorn

Majeska

2002

Journal Orthopaedic Research

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Estrogen has been reported to regulate the growth and differentiation of cultured murine osteoprogenitor cells in bone marrow stroma. This study tested the ability of 17P-estradiol (E2) to regulate growth and expression of alkaline phosphatase (ALP), an osteoblastic differentiation marker, in strains of normal human bone marrow stromal cells derived from different donors. In eight strains examined, E2 at 1 and 10 n M produced at most modest effectxs on growth and ALP activity. Growth inhibition, seen in 4 of the 8 strains, was more common than stimulation (2 of the 8 strains); the greatest observed E2 effect was a n inhibition of ca. 5 0 V~ E2 altered ALP activity less dramatically than cell growth. Differences from control in total ALP per culture were seen in only two strains; one was a reduction, one an increase. Colony forming assays were used to determine if E2 changed the proportion of ALPexpressing cells in marrow stromal cell cultures. In contrast to growth experiments, ALP expression under colony forming conditions (200 cells per 35 mm-diameter well) was dependent on the type of serum supplementation used. Under permissive conditions using medium supplemented with 10% charcoal-treated fetal bovine serum, 10 n M E2 increased the number of ALP-positive colonies (cfu-ap) but not the total number of colonies formed (cfu-f). When cells cultured in the presence or absence of 10 nM E2 were replated at colony forming densities, significantly higher proportions of cfu-ap were found in 2 of 6 strains examined, while pretreatment with E2 affected the number of cfu-f in only 1 of the 6 strains. Similar results were obtained when colony formation was carried out in the presence of dexamethasone and ascorbate, although these agents themselves increased the formation of both cfu-f and cfu-ap. These results show that the direct effects of E2 on human marrow stromal cells are small and vary depending on the cell strain and on the experimental conditions; however, the E2 actions observed in this study were consistent with reports that E2 exerts direct actions on osteoblasts and osteoblast progenitor cells that favor rather than suppress their phenotypic expression.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 90%

See 1 more Smart Citation

Estrogen regulation of growth and alkaline phosphatase expression by cultured human bone marrow stromal cells

Holzer

Einhorn

Majeska

2002

Journal Orthopaedic Research

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“…It is known that the proliferation and differentiation both in vitro as well as in vivo of MSC in osteoblasts are under the command of several factors (Lindblad, 2001) such as genes, growth factors, mechanical stimulus and some hormones (Payushina et al, 2006). These factors may act directly or indirectly in the various phases of osteogenic differentiation (Qu et al, 1998;Hughes et al, 2006) and little is known about the role of the PRL in each one of these steps and if such action is dose-dependent. Our results demonstrate that the action of prolactin in vitro on the MSC seems to be different from the action of this hormone in cultures of osteoblasts, despite of having been used concentrations similar to prolactin.…”

Section: Discussionmentioning

confidence: 99%

Osteogenic differentiation of adipose tissue-derived mesenchymal stem cells cultured with different concentrations of prolactin

Oliveira

Reis

Silva

et al. 2017

Arq. Bras. Med. Vet. Zootec.

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The objective was to evaluate the in vitro effect of prolactin in osteogenic potential of adipose tissue-derived mesenchymal stem cells (ADSCs) in female rats. ADSCs were cultured in osteogenic medium with and without the addition of prolactin and distributed into three groups: 1) ADSCs (control), 2) ADSCs with addition of 100ng/mL of prolactin and 3) ADSCs with addition of 300ng/mL of prolactin. At 21 days of differentiation, the tests of MTT conversion into formazan crystals, percentage of mineralized nodules and cells per field and quantification of genic transcript for alkaline phosphatase, osteopontin, osteocalcin, bone sialoprotein, BMP-2 and collagen I by real-time RT-PCR were made. The addition of prolactin reduced the conversion of MTT in group 3 and increased the percentage of cells per field in the groups 2 and 3, however without significantly increasing the percentage of mineralized nodules and the expression of alkaline phosphatase, osteopontin, osteocalcin, bone sialoprotein, BMP-2 and collagen I. In conclusion, the addition of prolactin in concentrations of 100ng/mL and 300ng/mL does not change the osteogenic differentiation to the ADSCs of female rats despite increase in the cellularity of the culture.

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“…The actual mechanism of enhancement of proliferation and growth factor production induced by the presence of E2 is still under investigation. A possible explanation is that the interaction between E2 and estrogen receptor plays an important role in regulating ADSC behaviors (Qu et al 1998;Oreffo et al 1999;Yeh et al 1999;Zhou et al 2001). The adipogenic differentiation of ADSCs is of great significance in controlling in vivo adipose tissue regeneration after transplantation.…”

Section: Discussionmentioning

confidence: 99%

Adipose Tissue-Derived Stem Cells Treated with Estradiol Enhance Survival of Autologous Fat Transplants

Luo

Hao

et al. 2013

Tohoku J. Exp. Med.

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Autologous fat transplantation (AFT) is a common and important operation in plastic surgery for soft tissue defects and adipose tissue-derived stem cells (ADSCs) are considered as a promising supplement to decrease absorption and subsequent side effects due to the ability of multiple differentiation and production of vascular endothelial growth factor (VEGF). The capacities of ADSCs can be further enhanced by treatment with 17-β estradiol (E2). Therefore, we hypothesized that E2 may promote the potential of ADSCs for AFT. In this study, ADSCs were extracted from three female patients by liposuction. In vitro studies showed that E2 supplementation at an optimal concentration of 10 -8 M resulted in enhanced proliferation, VEGF production, and adipogenic differentiation of human ADSCs, and reduced apoptosis rate in a serum-free environment. In addition, a nude mice model of fat transplantation was utilized to demonstrate the efficacy of ADSC for survival ratio in vivo. These results using the volume of fat tissues after 12 weeks compared original volume, revealed that the addition of E2-treated ADSCs induced a significantly higher tissue survival ratio (76.9 ± 1.9%) when compared with the ADSC-free system (55.5 ± 1.5%). Furthermore, increased capillary formation stained with hematoxylin-eosin (H&E) was observed in ADSCs systems after treatment with E2. Therefore, this study demonstrated E2 could promote the capacities of ADSCs about aspects of adipogenic differentiation, growth factor secretion and apoptosis reduction in vitro, vascularization improvement in vivo, and then enhanced the survival ratio of AFT.

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Estrogen enhances differentiation of osteoblasts in mouse bone marrow culture

Cited by 228 publications

References 35 publications

Estrogen regulation of growth and alkaline phosphatase expression by cultured human bone marrow stromal cells

Estrogen regulation of growth and alkaline phosphatase expression by cultured human bone marrow stromal cells

Osteogenic differentiation of adipose tissue-derived mesenchymal stem cells cultured with different concentrations of prolactin

Adipose Tissue-Derived Stem Cells Treated with Estradiol Enhance Survival of Autologous Fat Transplants

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