Estrogen and progestin receptors in endometriosis lesions: Comparison with endometrial tissue

Jänne, Olli A.; Kauppila, Antti; Kokko, E.; Lantto, T.; Rönnberg, Lars; Vihko, R.

doi:10.1016/s0002-9378(15)33278-6

Cited by 134 publications

(34 citation statements)

References 14 publications

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“…Moreover, the importance of steroid hormones, such as estrogen and progesterone, and their receptors for the functioning and health of the adult uterus has been shown in numerous studies investigating endometriosis [39-41], leiomyomas [42-44], endometrial cancer [45, 46], and recurrent pregnancy loss [47, 48]. …”

Section: Discussionmentioning

confidence: 99%

Decidualization is Impaired in Endometrial Stromal Cells from Uterine Rudiments in Mayer-Rokitansky-Küster-Hauser Syndrome

Brucker

Eisenbeis

König

et al. 2017

Cell Physiol Biochem

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Background/Aims: Uterine rudiments from patients with Mayer-Rokitansky-Küster-Hauser syndrome (MRKHS) contain all tissues typically found in the uterus. Endometrium from the rudiments predominantly exhibits basalis-like features, and endometrial proliferative capacity in patients’ epithelium and stroma is significantly lower. Methods: This single-center, prospective study conducted at a major German university hospital compared in-vitro decidualization in cultured ESCs from MRKHS patients and hysterectomy controls. Primary ESC cultures were established from both sources. Hormone-induced prolactin and IGFBP-1 secretion served as a measure of their ability to undergo decidualization in response to hormonal stimulation. Expression levels of 8 key marker genes of decidualization were also determined. Results: At day 9, mean secretion of prolactin and IGFBP-1 was significantly reduced by 89.0% and 99.5%, respectively, in MRKHS ESCs vs. hysterectomy controls, both indicating impaired decidualization of MRKHS ESCs. Key decidual markers confirmed impaired decidualization in MRKHS patients. Conclusion: Our results indicate that the ESCs from MRKHS patients lack hormone responsiveness as a potential sign of dysfunctional hormone receptor function, which may also play a role in the onset of MRKHS. Further studies are needed to corroborate our findings, directly address receptor function, and elucidate the role of other potential determinants of uterine development and adult function.

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Section: Discussionmentioning

confidence: 99%

Decidualization is Impaired in Endometrial Stromal Cells from Uterine Rudiments in Mayer-Rokitansky-Küster-Hauser Syndrome

Brucker

Eisenbeis

König

et al. 2017

Cell Physiol Biochem

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“…Relative growth inhibition of the implants has been attributed to a suboptimal extrauterine environment and/or mitogenic interference by inflammatory cytokines (Zarmakoupis et al, 1995). It has been reported that the concentrations of oestrogen (ER) and progestin receptors (PR) in endometriosis lesion homogenates were lower than in extracts from normal eutopic endometrium (Jänne et al, 1981). However, a caveat of the observation is that peritoneal cells associated with endometriosis implants are ER-and PRnegative and would be expected to dilute receptor concentrations in these homogenates (Prentice et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Oestrogen receptor (ER)-alpha and ER- isoforms in normal endometrial and endometriosis-derived stromal cells

Brandenberger

Lebovic

Tee

et al. 1999

Molecular Human Reproduction

154

108

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Several investigators have noted that hormone-dependent development of endometriosis implants lags behind that of simultaneously analysed eutopic endometrium. With the recent discovery of the oestrogen receptor-β (ER-β) isoform, the aim of this study was to investigate whether differences in the expression of ER-α and ER-β might explain this observation. mRNA transcripts from endometrial stromal cells isolated from normal endometrium (NE) and from endometriomas (EI) were analysed using a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) technique. RT-PCR and Southern blot analyses of the two ER isoforms indicated that NE and EI stromal cells predominantly express ER-α mRNA, however the relative concentrations of ER isoform mRNA transcripts differed between the two cell types. Steady-state ER-α:ER-β mRNA ratios were 15.5 ⍨ 2.8 and 5.2 ⍨ 0.9 respectively for NE and EI cells (P ⍧ 0.02). NE and EI stromal cells expressed ER proteins with similar K d (~0.9 nM) and densities (~24 500 binding sites/cell) respectively. Functional ER expression was indicated by an increase in progesterone receptor concentrations of~60% (P ⍧ 0.03) after incubation with 10 nM oestradiol. We postulate that differential transcript processing, ligand specificity and biological actions of the ER-α and -β isoforms may influence differential growth responses in normal and ectopic endometrium.

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“…Immunohistochemically, in the series reported by Flieder and associates, most glands showed cytoplasmic positivity with broad spectrum cytokeratin, cytokeratin 7, and BER-EP4, and strong nuclear staining for estrogen and progesterone receptors [8]. Estrogen and progesterone receptors are present in endometriotic glands and stroma in a lower concentration than in eutopic endometrium [10,11]. Like normal and neoplastic endometrial stromal cells, endometriotic stromal cells are typically immunoreactive for CD10 [9].…”

Section: Discussionmentioning

confidence: 99%

Pleural Endometriosis: An Exceptional Cause of Hemorrhagic Pleural Effusion

Bhattacharjee

Deb

Saha

et al. 2013

J Obstet Gynecol India

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Estrogen and progestin receptors in endometriosis lesions: Comparison with endometrial tissue

Cited by 134 publications

References 14 publications

Decidualization is Impaired in Endometrial Stromal Cells from Uterine Rudiments in Mayer-Rokitansky-Küster-Hauser Syndrome

Decidualization is Impaired in Endometrial Stromal Cells from Uterine Rudiments in Mayer-Rokitansky-Küster-Hauser Syndrome

Oestrogen receptor (ER)-alpha and ER- isoforms in normal endometrial and endometriosis-derived stromal cells

Pleural Endometriosis: An Exceptional Cause of Hemorrhagic Pleural Effusion

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