Estradiol treatment decreases cell proliferation in the neurogenic zones of adult female zebrafish (Danio rerio) brain

Makantasi, P.; Dermon, Catherine R.

doi:10.1016/j.neuroscience.2014.06.071

Cited by 29 publications

(25 citation statements)

References 106 publications

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“…In agreement with previous studies in other teleost species [36][37], a populations of mitotically active cells were found to express the early neuronal marker TUC-4, within Dm, PGZ and VCe M (double-labeled BrdU-TUC-4), or BLBP stem cells (BrdU-BLBP) in PGZ and Dm.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Early life low intensity stress experience modifies acute stress effects on juvenile brain cell proliferation of European sea bass ( D. Labrax )

Fokos

Pavlidis

Yiotis

et al. 2017

Behavioural Brain Research

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Section: Discussionsupporting

confidence: 92%

“…To determine the phenotype of proliferating cells double immunofluorescence experiments were performed in adjacent sections, using an early neuronal marker, (Turned On After Division, TOAD, TUC-4) and a glial marker brain lipid binding protein (BLBP) [36][37]. …”

Section: Double Immunofluorescencementioning

confidence: 99%

Early life low intensity stress experience modifies acute stress effects on juvenile brain cell proliferation of European sea bass ( D. Labrax )

Fokos

Pavlidis

Yiotis

et al. 2017

Behavioural Brain Research

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“…In zebrafish, estrogens inhibit the activity of aromatase and Pcna during repair of brain lesion in adult (Diotel et al, ). The inhibitory E 2 effects were found on neurogenesis in the optic tectume of sexually mature female medaka (Takeuchi and Okubo, ) and on the proliferation in brain cells in adult female zebrafish (Makantasi and Dermon, ). These data suggest a complex role for E 2 in the brain neurogenesis and development which may vary according to developmental stage, age, physiological status, treatment, and species.…”

Section: Discussionmentioning

confidence: 99%

Activation of brain steroidogenesis and neurogenesis during the gonadal differentiation in protandrous black porgy, Acanthopagrus schlegelii

Lin

Fan-Chiang

Dufour

et al. 2015

Developmental Neurobiology

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The early brain development, at the time of gonadal differentiation was investigated using a protandrous teleost, black porgy. This natural model of monosex juvenile fish avoids the potential complexity of sexual dimorphism. Brain neurogenesis was evaluated by histological analyses of the diencephalon, at the time of testicular differentiation (in fish between 90 and 150 days after hatching). Increases in the number of both Nissl-stained total brain cells, and Pcna-immunostained proliferative brain cells were observed in specific area of the diencephalon, such as ventromedialis thalami and posterior preoptic area, revealing brain cell proliferation. qPCR analyses showed significantly higher expression of the radial glial cell marker blbp and neuron marker bdnf. Strong immunohistochemical staining of Blbp and extended cellular projections were observed. A peak expression of aromatase (cyp19a1b), as well as an increase in estradiol (E2 ) content were also detected in the early brain. These data demonstrate that during gonadal differentiation, the early brain exhibits increased E2 synthesis, cell proliferation, and neurogenesis. To investigate the role of E2 in early brain, undifferentiated fish were treated with E2 or aromatase inhibitor (AI). E2 treatment upregulated brain cyp19a1b and blbp expression, and enhanced brain cell proliferation. Conversely, AI reduced brain cell proliferation. Castration experiment did not influence the brain gene expression patterns and the brain cell number. Our data clearly support E2 biosynthesis in the early brain, and that brain E2 induces neurogenesis. These peak activity patterns in the early brain occur at the time of gonad differentiation but are independent of the gonads.

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“…Cell survival was also slightly decreased at the junction between the olfactory bulbs after long-term treatment with estradiol, but these results requires further investigation [32]. Interestingly, the role of estradiol in neurogenesis has been addressed in adult female zebrafish and, as in male, 17b estradiol induces a region-specific decrease in the number of cycling cells especially in telencephalic, hypothalamic and cerebellar areas [114]. In order to highlight the potential role of estradiol in regenerative neurogenesis, we have developed a model of mechanical lesion of the telencephalon.…”

Section: Estrogens Effects On Constitutive and Reparative Neurogenesimentioning

confidence: 99%

Steroid modulation of neurogenesis: Focus on radial glial cells in zebrafish

Pellegrini

Diotel

Vaillant-Capitaine

et al. 2016

The Journal of Steroid Biochemistry and Molecular Biology

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Estrogens are known as steroid hormones affecting the brain in many different ways and a wealth of data now document effects on neurogenesis. Estrogens are provided by the periphery but can also be locally produced within the brain itself due to local aromatization of circulating androgens. Adult neurogenesis is described in all vertebrate species examined so far, but comparative investigations have brought to light differences between vertebrate groups. In teleost fishes, the neurogenic activity is spectacular and adult stem cells maintain their mitogenic activity in many proliferative areas within the brain. Fish are also quite unique because brain aromatase expression is limited to radial glia cells, the progenitor cells of adult fish brain. The zebrafish has emerged as an interesting vertebrate model to elucidate the cellular and molecular mechanisms of adult neurogenesis, and notably its modulation by steroids. The main objective of this review is to summarize data related to the functional link between estrogens production in the brain and neurogenesis in fish. First, we will demonstrate that the brain of zebrafish is an endogenous source of steroids and is directly targeted by local and/or peripheral steroids. Then, we will present data demonstrating the progenitor nature of radial glial cells in the brain of adult fish. Next, we will emphasize the role of estrogens in constitutive neurogenesis and its potential contribution to the regenerative neurogenesis.Finally, the negative impacts on neurogenesis of synthetic hormones used in contraceptive pills production and released in the aquatic environment will be discussed.

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Estradiol treatment decreases cell proliferation in the neurogenic zones of adult female zebrafish (Danio rerio) brain

Cited by 29 publications

References 106 publications

Early life low intensity stress experience modifies acute stress effects on juvenile brain cell proliferation of European sea bass ( D. Labrax )

Early life low intensity stress experience modifies acute stress effects on juvenile brain cell proliferation of European sea bass ( D. Labrax )

Activation of brain steroidogenesis and neurogenesis during the gonadal differentiation in protandrous black porgy, Acanthopagrus schlegelii

Steroid modulation of neurogenesis: Focus on radial glial cells in zebrafish

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