Estradiol stimulates cell proliferation via classic estrogen receptor-alpha and G protein-coupled estrogen receptor-1 in human renal tubular epithelial cell primary cultures

Sanchez, Daiana Soledad; Sigel, Lilian Karina Fischer; Azurmendi, Pablo Javier; Vlachovsky, Sandra G.; Oddo, Elisabet Mónica; Armando, Inés; Ibarra, Fernando; Silberstein, Claudia

doi:10.1016/j.bbrc.2019.03.056

Cited by 11 publications

(10 citation statements)

References 32 publications

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“…Interestingly, these parameters were not altered in our model, suggesting a partial inter-species difference. Gender contributes to differences in incidence and progression of CKD, and some rat models, such as adenine-induced CKD, displayed differences, with males being more severely affected than females [21], at least partially due to estrogen/estrogen receptors [22,23]. After a dose-response study, which allowed us to choose an oxalate concentration of 50 µmol/g (Figure A1), all of our experiments were performed on males, excluding one comparative experiment that may have shown that both male and females were sensitive to the OXA diet; this is despite there not being a significant trend for more severe phenotypes in females (Figure A2), suggesting that gender does not influence oxalate nephrotoxicity, which is a major advantage of our protocol.…”

Section: Discussionmentioning

confidence: 99%

A Sodium Oxalate-Rich Diet Induces Chronic Kidney Disease and Cardiac Dysfunction in Rats

Crestani

Crajoinas

Jensen

et al. 2021

IJMS

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Chronic kidney disease (CKD) is a worldwide public health issue affecting 14% of the general population. However, research focusing on CKD mechanisms/treatment is limited because of a lack of animal models recapitulating the disease physiopathology, including its complications. We analyzed the effects of a three-week diet rich in sodium oxalate (OXA diet) on rats and showed that, compared to controls, rats developed a stable CKD with a 60% reduction in glomerular filtration rate, elevated blood urea levels and proteinuria. Histological analyses revealed massive cortical disorganization, tubular atrophy and fibrosis. Males and females were sensitive to the OXA diet, but decreasing the diet period to one week led to GFR significance but not stable diminution. Rats treated with the OXA diet also displayed classical CKD complications such as elevated blood pressure and reduced hematocrit. Functional cardiac analyses revealed that the OXA diet triggered significant cardiac dysfunction. Altogether, our results showed the feasibility of using a convenient and non-invasive strategy to induce CKD and its classical systemic complications in rats. This model, which avoids kidney mass loss or acute toxicity, has strong potential for research into CKD mechanisms and novel therapies, which could protect and postpone the use of dialysis or transplantation.

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Section: Discussionmentioning

confidence: 99%

A Sodium Oxalate-Rich Diet Induces Chronic Kidney Disease and Cardiac Dysfunction in Rats

Crestani

Crajoinas

Jensen

et al. 2021

IJMS

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“…KGN cell line was established from a human ovarian granulosa cell tumour highly expressing ERβ 96 . Human renal tubular epithelial cells (HRTEC) express GPER 97 .…”

Section: Methodsmentioning

confidence: 99%

Molecular mechanisms underlying AMH elevation in hyperoestrogenic states in males

Valeri

Lovaisa

Racine

et al. 2020

Sci Rep

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Anti-Müllerian hormone (AMH) is secreted by Sertoli cells of the testes from early fetal life until puberty, when it is downregulated by androgens. In conditions like complete androgen insensitivity syndrome (CAIS), AMH downregulation does not occur and AMH increases at puberty, due in part to follicle-stimulating hormone (FSH) effect. However, other conditions like Peutz-Jeghers syndrome (PJS), characterised by low FSH, also have increased AMH. Because both CAIS and PJS may present as hyperoestrogenic states, we tested the hypothesis that oestradiol (E2) upregulates AMH expression in peripubertal Sertoli cells and explored the molecular mechanisms potentially involved. The results showed that E2 is capable of inducing an upregulation of endogenous AMH and of the AMH promoter activity in the prepubertal Sertoli cell line SMAT1, signalling through ERα binding to a specific ERE sequence present on the hAMH promoter. A modest action was also mediated through the membrane oestrogen receptor GPER. Additionally, the existence of ERα expression in Sertoli cells in patients with CAIS was confirmed by immunohistochemistry. The evidence presented here provides biological plausibility to the hypothesis that testicular AMH production increases in clinical conditions in response to elevated oestrogen levels.

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“…110 In human renal tubular epithelial cells, E2 leads to cell proliferation via ERα and GPER-1. 111 In vitro experiments showed that ERβ suppressed the transcriptional and oncogenic effects of ERα. 112,113 The functions of ERα and ERβ are antagonistic; therefore, their ratio is important in the development of diseases.…”

Section: Discussionmentioning

confidence: 99%

<p>ERα, A Key Target for Cancer Therapy: A Review</p>

Liu

Yao

2020

OTT

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Estrogen receptor α (ERα) is closely associated with both hormone-dependent and hormone-independent tumors, and it is also essential for the development of these cancers. The functions of ERα are bi-faceted; it can contribute to cancer progression as well as cancer inhibition. Therefore, understanding ERα is vital for the treatment of those cancers that are closely associated with its expression. Here, we will elaborate on ERα based on its structure, localization, activation, modification, and mutation. Also, we will look at coactivators of ERα, elucidate the signaling pathway activated by ERα, and identify cancers related to its activation. A comprehensive understanding of ERα could help us to find new ways to treat cancers.

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Estradiol stimulates cell proliferation via classic estrogen receptor-alpha and G protein-coupled estrogen receptor-1 in human renal tubular epithelial cell primary cultures

Cited by 11 publications

References 32 publications

A Sodium Oxalate-Rich Diet Induces Chronic Kidney Disease and Cardiac Dysfunction in Rats

A Sodium Oxalate-Rich Diet Induces Chronic Kidney Disease and Cardiac Dysfunction in Rats

Molecular mechanisms underlying AMH elevation in hyperoestrogenic states in males

<p>ERα, A Key Target for Cancer Therapy: A Review</p>

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