Estradiol regulates large dense core vesicles in the hippocampus of adult female rats

May, Renee M.; Tabatadze, Nino; Czech, Mary M; Woolley, Catherine S.

doi:10.1007/s00429-013-0614-7

Cited by 7 publications

(4 citation statements)

References 42 publications

(54 reference statements)

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“…These data suggest that, under conditions of increased NPY expression, NPY release may not require high-frequency activity and can be tonically released. Indeed, a study investigating E 2mediated effects on NPY release in the CA1 hippocampal region found that enhanced NPY release is related to the increase in NPY cellular content (64). We did not find significant differences in NPY release between PPT and DPN treatments, which did not reflect the difference in the number of NPY immunopositive neurones.…”

Section: Discussioncontrasting

confidence: 70%

Oestradiol Regulates Neuropeptide Y Release and Gene Coupling with the GABAergic and Glutamatergic Synapses in the Adult Female Rat Dentate Gyrus

Velı́šková

Iacobaş

et al. 2015

J Neuroendocrinology

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Neuropeptide Y (NPY) is an endogenous modulator of neuronal activity affecting both GABAergic and glutamatergic transmission. Previously, we found that oestradiol modifies the number of NPY immunoreactive neurones in the hippocampal dentate gyrus. In the present study, we investigated which oestrogen receptor type is responsible for these changes in the number of NPY-positive neurones. Furthermore, we determined the effects of oestrogen receptor activation on NPY release. Finally, we examined the contribution of oestrogen toward the remodelling of the GABAergic and glutamatergic gene networks in terms of coupling with Npy gene expression in ovariectomised rats. We found that activation of either oestrogen receptor type (ERα or ERβ) increases the number of NPY-immunopositive neurones and enhances NPY release in the dentate gyrus. We also found that, compared to oestrogen-lacking ovariectomised rats, oestrogen replacement increases the probability of synergistic/antagonistic coupling between the Npy and GABAergic synapse genes, whereas the glutamatergic synapse genes are less likely to be coupled with Npy under similar conditions. The data together suggest that oestrogens play a critical role in the regulation of NPY system activity and are also involved in the coupling/uncoupling of the Npy gene with the GABAergic and glutamatergic synapses in the female rat dentate gyrus.

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Section: Discussioncontrasting

confidence: 70%

Oestradiol Regulates Neuropeptide Y Release and Gene Coupling with the GABAergic and Glutamatergic Synapses in the Adult Female Rat Dentate Gyrus

Velı́šková

Iacobaş

et al. 2015

J Neuroendocrinology

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“…On the other hand, deletion of ERα expression in GABAergic, but not glutamatergic hippocampal neurons has a pronounced effect on estrogen-dependent behavioral masculinization in the mouse 72 . Moreover, estradiol enhances the release probability of dense core vesicles 73 , which could provide a mechanism of increased excitability due to convergent projections of OR, AVP, DA, and 5-HT.…”

Section: Discussionmentioning

confidence: 99%

A GABAergic cell type in the lateral habenula links hypothalamic homeostatic and midbrain motivation circuits with sex steroid signaling

et al. 2018

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The lateral habenula (LHb) has a key role in integrating a variety of neural circuits associated with reward and aversive behaviors. There is limited information about how the different cell types and neuronal circuits within the LHb coordinate physiological and motivational states. Here, we report a cell type in the medial division of the LHb (LHbM) in male rats that is distinguished by: (1) a molecular signature for GABAergic neurotransmission (Slc32a1/VGAT) and estrogen receptor (Esr1/ERα) expression, at both mRNA and protein levels, as well as the mRNA for vesicular glutamate transporter Slc17a6/VGLUT2, which we term the GABAergic estrogen-receptive neuron (GERN); (2) its axonal projection patterns, identified by in vivo juxtacellular labeling, to both local LHb and to midbrain modulatory systems; and (3) its somatic expression of receptors for vasopressin, serotonin and dopamine, and mRNA for orexin receptor 2. This cell type is anatomically located to receive afferents from midbrain reward (dopamine and serotonin) and hypothalamic water and energy homeostasis (vasopressin and orexin) circuits. These afferents shared the expression of estrogen synthase (aromatase) and VGLUT2, both in their somata and axon terminals. We demonstrate dynamic changes in LHbM VGAT+ cell density, dependent upon gonadal functional status, that closely correlate with motivational behavior in response to predator and forced swim stressors. The findings suggest that the homeostasis and reward-related glutamatergic convergent projecting pathways to LHbMC employ a localized neurosteroid signaling mechanism via axonal expression of aromatase, to act as a switch for GERN excitation/inhibition output prevalence, influencing depressive or motivated behavior.

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“…In ER-positive breast cancer, the ER receptor is responsible for the proliferative and growth effects of 17β-estradiol (E 2 ), the primary estrogenic hormone, through a combination of genomic and nongenomic actions that have been described at different cell levels, including the plasma membrane 37,38. The effects of estrogens on vesicle formation via the ER were previously observed in different systems39,40,41,42 related to lysosomal function in human breast cancer and hepatocarcinoma cell lines,43–46 suggesting the involvement of a type of receptor-mediated endocytosis. The mechanism of action of this phenomenon is unclear.…”

Section: Introductionmentioning

confidence: 99%

<p>Gold nanoparticle uptake is enhanced by estradiol in MCF-7 breast cancer cells</p>

Lara-Cruz

Jiménez-Salazar

Arteaga

et al. 2019

IJN

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Purpose: In the present study, we investigated the effects of 17β-estradiol (E 2 ) on membrane roughness and gold nanoparticle (AuNP) uptake in MCF-7 breast cancer cells. Methods: Estrogen receptor (ER)-positive breast cancer cells (MCF-7) were exposed to bare 20 nm AuNPs in the presence and absence of 1×10 −9 M E 2 for different time intervals for up to 24 hrs. The effects of AuNP incorporation and E 2 incubation on the MCF-7 cell surface roughness were measured using atomic force microscopy (AFM). Endocytic vesicle formation was studied using confocal laser scanning microscopy (CLSM). Finally, the results were confirmed by hyperspectral optical microscopy. Results: High-resolution AFM images of the surfaces of MCF-7 membranes (up to 250 nm 2 ) were obtained. The incubation of cells for 12 hrs with AuNP and E 2 increased the cell membrane roughness by 95% and 30% compared with the groups treated with vehicle (ethanol) or AuNPs only, respectively. This effect was blocked by an ER antagonist (7α,17β-[9-[(4,4,5,5,5-Pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-triene-3,17-diol [ICI] 182,780). Higher amounts of AuNPs were localized inside MCF-7 cells around the nucleus, even after 6 hrs of E 2 incubation, compared with vehicle-treated cells. Endolysosome formation was induced by E 2 , which may be associated with an increase in AuNP-uptake. Conclusions: E 2 enhances AuNP incorporation in MCF-7 cells by modulating of plasma membrane roughness and inducing lysosomal endocytosis. These findings provide new insights into combined nanotherapies and hormone therapies for breast cancer.

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Estradiol regulates large dense core vesicles in the hippocampus of adult female rats

Cited by 7 publications

References 42 publications

Oestradiol Regulates Neuropeptide Y Release and Gene Coupling with the GABAergic and Glutamatergic Synapses in the Adult Female Rat Dentate Gyrus

Oestradiol Regulates Neuropeptide Y Release and Gene Coupling with the GABAergic and Glutamatergic Synapses in the Adult Female Rat Dentate Gyrus

A GABAergic cell type in the lateral habenula links hypothalamic homeostatic and midbrain motivation circuits with sex steroid signaling

<p>Gold nanoparticle uptake is enhanced by estradiol in MCF-7 breast cancer cells</p>

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