Estradiol of various saturation and its influence on hydrogen sulfide formation in the myocardium of female rats.

Melnik, A. V.

doi:10.26641/2307-0404.2015.1.40233

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The H 2 S content in the post-nuclear supernatant was determined using a spectrophotometric method by reaction with N, N-dimethyl-para-phenylenediamine in the presence of FeCl 3 [19]. The activity of H 2 Ssynthesizing enzymes -сystathionine gamma-lyase (CSE, EC 4.4.1.1), сystathionine beta-synthase (CBS, EC 4.2.1.22), and cysteine aminotransferase (CAT, EC 2.6.1.3) was evaluated according to the growth of a sulfide anion in the incubation medium as adapted by our group [20]. Concentrations of substrates and cofactors, pH, and duration of incubation provided optimal conditions for determining the activity of the enzymes selected a priori.…”

Section: Biochemical and Functional Measurementsmentioning

confidence: 99%

Hydrogen Sulfide Metabolism and Its Role in Kidney Function in a Rat Model of Chronic Kidney Disease

Koniukh¹,

Волощук²,

Melnyk³

et al. 2020

hpc

View full text Add to dashboard Cite

Authors' contribution Wkład autorów:A. Study design/planning zaplanowanie badań B. Data collection/entry zebranie danych C. Data analysis/statistics dane -analiza i statystyki D. Data interpretation interpretacja danych E. Preparation of manuscript przygotowanie artykułu F. Literature analysis/search wyszukiwanie i analiza literatury G. Funds collection zebranie funduszy Summary Background. Chronic kidney disease (CKD) is an ongoing global problem. It is correlated with a substantial increase in mortality and morbidity. Although, hydrogen sulfide (H 2 S) plays an important role in the physiological and pathological processes in the kidney, the influence of CKD on the enzymatic synthesis and utilization of H 2 S in the kidney is unclear. The aim of this study was to evaluate the activity of H 2 S-producing enzymes (сystathionine gamma-lyase, cystathionine beta-synthase and cysteine aminotransferase) and the content of H 2 S in rats with CKD, and to establish the relationship between these parameters and markers of the functional state of the kidneys. Material and methods. CKD in rats was induced by 5/6 nephrectomy of the contralateral kidney. H 2 S regulation was examined in post-nuclear kidney homogenates by measuring H 2 S levels and cystathionine gamma-lyase, cystathionine beta-synthase, and cysteine aminotransferase activity using spectrophotometric methods. Functional and biochemical measurements were monitored after water load (5% of body mass). Results. CKD in rats was associated with defects in H 2 S metabolism in rat kidneys. The activity of H 2 Sproducing enzymes decreased by 28.3-34.2% (p<0.05), the rate of utilization of exogenous H 2 S in the kidneys increased by 34.3% (p<0.05), and the content of H 2 S was reduced by 35.8% (p<0.05) in comparison with a control group. A progressive loss of kidney function (tubular and glomerular disorders) is closely correlated with the content of H 2 S in the kidneys. Conclusions. The H 2 S system in kidneys may be an important metabolic target, which can influence the efficacy of treatments to improve the functional state of kidneys in CKD.

show abstract

Section: Biochemical and Functional Measurementsmentioning

confidence: 99%